Preparing The Qc Sample; Required Materials; Cytoflex Daily Qc Fluorospheres Preparation Process - Beckman Coulter CytoFLEX SRT Instructions For Use Manual

Notifies you if laser delay is > 5 μs from the previous setting. Manual laser delay adjustments
are required. Refer to
Procedures.
6. Verifies and calibrates the gain settings. If any of these parameters are outside of the operating
limits, the system automatically adjusts these parameters. If the system is unable to adjust
these parameters to fall within the operating limits, the system notifies you.
7. Optimize the fluid stream and verify whether the jitter of T1 signal is acceptable. Then
determine the DD1 value.

Preparing the QC Sample

Required Materials

The following materials are required to complete the QC process:
• CytoFLEX Daily QC Fluorospheres or CytoFLEX Ready to Use Daily QC Fluorospheres
• IsoFlow Sheath Fluid or another saline sheath fluid
• Sample tubes (12 x 75 mm)
• Vortexer

CytoFLEX Daily QC Fluorospheres Preparation Process

IMPORTANT
CytoFLEX Ready to Use Daily QC Fluorospheres Preparation
1
Take one sample tube and label it as the QC sample tube.
2
Add approximately 1 mL of deionized water to the sample tube.
3
Use the Vortexer or shake vigorously to thoroughly mix the bottle of CytoFLEX Daily QC
Fluorospheres.
4
Add three drops of CytoFLEX Daily QC Fluorospheres to the sample tube.
5
Vortex the sample tube to uniformly suspend the fluorospheres.
C37808AC
Setting Laser Delay
For preparation instructions for the CytoFLEX Ready to Use Daily QC Fluorospheres, refer to
Instrument Quality Control and Standardization
in CHAPTER 11, Replacement/Adjustment
Process.
4
Quality Control
4-3