Calibrating The Instrument For Mikes And Nrms Experiments; Calibrating The Instrument For Scanning Experiments - Waters Autospec Premier Operator's Manual

10. When the peaks are acceptable, in the Experiment Calibration dialog
box, click Continue to end the calibration and create a calibration file
(*.sircal) for the experiment.
If you are using a multi-function experiment for the calibration,
Note:
clicking Continue advances you to the next function.
Calibrating the instrument for MIKES and NRMS
experiments
Use the experiment calibration dialog box to calibrate MIKES and NRMS
experiments. In these types of acquisition, a parent ion is selected by ESA1
and the magnet. It is fragmented in the third field-free region (FFR3, between
the magnet and ESA2). ESA2 is scanned to form an energy spectrum of the
fragment ions.
Although this is a scanning technique, an SIR calibration is required to
accurately select the parent ion.

Calibrating the instrument for scanning experiments

You can calibrate scanning experiments after acquisition. Scan to slightly
higher and lower masses than the mass window of interest to ensure that
masses of interest do not fall outside the mass window of the experiment.
Recommendation:
experiment.
To perform a scanning calibration
1. On the MassLynx window's Sample List menu bar, click Spectrum.
2. In the Spectrum window, click
Alternative:
3. In the Spectrum Data Browser dialog box, select the required data file
and click OK.
4. In the Spectrum window, click Tools > Make Calibration.
5. In the Make New Calibration dialog box, select the reference file against
which to calibrate the data.
Perform a scanning calibration at least once for each
Click File > Open.

Calibrating the instrument for MIKES and NRMS experiments

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