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Waters ACQUITY UPLC I-Class IVD System Manual

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ACQUITY UPLC
I-Class IVD
System Guide
715003736IVD / Revision C
Copyright © Waters Corporation 2014
All rights reserved

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Summary of Contents for Waters ACQUITY UPLC I-Class IVD

  • Page 1 ACQUITY UPLC I-Class IVD System Guide 715003736IVD / Revision C Copyright © Waters Corporation 2014 All rights reserved...
  • Page 2 July 7, 2014, 715003736IVD Rev. C...

  • Page 3: General Information

    Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use. For the most recent revision of this document, consult the Waters Web site (waters.com).

  • Page 4: Contacting Waters

    Contacting Waters ® Contact Waters with technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail. Waters contact information: Contacting medium Information Internet The Waters Web site includes contact information for Waters locations worldwide.

  • Page 5: Considerations Specific To The Acquity Uplc I-Class Ivd Instruments

    Considerations specific to the ACQUITY UPLC I-Class IVD instruments To avoid injury, if the ACQUITY UPLC I-Class IVD system Warning: must be relocated, drain and remove all liquids, including solvents and waste, from the system. To avoid injury, if the ACQUITY UPLC I-Class IVD system Warning: must be relocated, disconnect the power cord and all cables.

  • Page 6: Safety Hazard Symbol Notice

    Operating the ACQUITY UPLC I-Class IVD system When operating the ACQUITY UPLC I-Class IVD system, follow standard quality control (QC) procedures and the guidelines presented in this section. Applicable symbols Symbol...

  • Page 7: Audience And Purpose

    European Union In Vitro Diagnostic Device Directive 98/79/EC. The ACQUITY UPLC I-Class IVD system is a general purpose in vitro diagnostic instrument used to quantify and qualify of a wide variety of compounds in biological sample matrices. The system is intended to be used only by qualified operators.

  • Page 8: Quality Control

    When analyzing samples from a complex matrix such as soil, tissue, serum/plasma, whole blood, and other sources, note that the matrix components can adversely affect LC/MS results, enhancing or suppressing ionization. To minimize these matrix effects, Waters recommends you adopt the following measures: •...

  • Page 9: Ism Classification

    Class B products are suitable for use in both commercial and residential locations and can be directly connected to a low voltage, power-supply network. EC authorized representative Waters Corporation Stamford Avenue Altrincham Road Wilmslow SK9 4AX United Kingdom...
  • Page 10 July 7, 2014, 715003736IVD Rev. C...

  • Page 11: Table Of Contents

    Trademarks ......................iii Contacting Waters ....................iv Safety considerations ..................iv Considerations specific to the ACQUITY UPLC I-Class IVD instruments ..v FCC radiation emissions notice ................v Canada spectrum management emissions notice ..........v Electrical power safety notice ................v Safety hazard symbol notice................
  • Page 12 Software and data system ................. 20 Optional columns ....................21 Vials, plates, and seals ..................22 For additional information ................22 2 Optimizing Performance ............... 23 General guidelines ..................... 23 Carryover ......................25 Contamination ....................26 Temperature ....................... 26 Reproducibility ....................26 Cycle time (between injections) ..............
  • Page 13 Warning symbols ....................49 Task-specific hazard warnings................49 Specific warnings ....................51 Caution advisory ....................53 Warnings that apply to all Waters instruments .......... 53 Electrical and handling symbols ..............59 Electrical symbols ....................59 Handling symbols ....................60 B Specifications ..................61 Physical specifications ..................
  • Page 14 Column Heater....................68 C External Connections ................69 System tubing connections ................69 External wiring connections ................71 ACQUITY UPLC I-Class IVD instrument external wiring connections ..71 Ethernet connections ..................72 Column heater connection................. 72 Signal connections ..................... 73 Connecting signal cables ...................
  • Page 15 Solvent viscosity ....................90 E FlexCart Assembly ................. 91 Assembling the FlexCart .................. 91 Adjusting the FlexCart’s height ..............93 Locking the FlexCart in place ................ 94 Moving the assembled FlexCart ..............95 July 7, 2014, 715003736IVD Rev. C...
  • Page 16 July 7, 2014, 715003736IVD Rev. C...

  • Page 17: Acquity Uplc I-Class Ivd System

    ACQUITY UPLC I-Class IVD System The ACQUITY UPLC I-Class IVD system is a general purpose, in vitro, tool for quantifying and qualifying diverse compounds in biological sample matrices. The system is intended to be used by only highly qualified operators.

  • Page 18: Binary Solvent Manager

    • FlexCart, optional Binary solvent manager The Waters ACQUITY UPLC I-Class IVD Binary Solvent Manager (BSM) delivers solvent compositions for isocratic and binary gradient methods at flow rates of between 0.01 and 2.0 mL per minute. Its features include in-line ®...

  • Page 19: Sample Manager-Flow Through Needle

    System components Sample manager-flow through needle The ACQUITY UPLC I-Class IVD Sample Manager - Flow Through Needle (SM - FTN) incorporates a flow-through-needle mechanism. The mechanism aspirates a sample from plates and vials and holds it in the sample needle in preparation for injecting the sample onto the column.

  • Page 20: Column Technology

    1 ACQUITY UPLC I-Class IVD System Column technology ACQUITY UPLC IVD columns are packed with sub-2-µm, bridged, ethylsiloxane, hybrid or 1.8-µm high-strength silica particles that can mechanically endure high-pressure conditions. The column hardware can withstand as much as 124,106 kPa (1241 bar, 18,000 psi). The column dimensions allow optimal MS-compatible flow rates, and matched outlet tubing minimizes the effect of extra-column volume.

  • Page 21: Optional Columns

    It securely transmits system usage, performance parameters, and diagnostic information to the Waters technical service team. Optional columns The standard column used with the ACQUITY UPLC I-Class IVD system is the ACQUITY UPLC BEH C column, 1.7 µm, 2.1 × 50 mm, IVD. The following additional columns may be used: •...

  • Page 22: Vials, Plates, And Seals

    Vials, plates, and seals For information on vials, plates, and seals, refer to the following: • Waters Autosampler Vials, Plates, and Seals for use with the ACQUITY UPLC Systems Family (part number 720004278). • Sample Vials and Accessories (part number 720001818).

  • Page 23: Optimizing Performance

    When performing fast UPLC analyses, note that a peak of interest can be less than 0.5 seconds wide. Waters recommends a sampling rate of 25 to 50 points across a peak, which provides good quantitation. Sampling rates faster than...
  • Page 24 2 Optimizing Performance 20 points per second yield higher baseline noise, requiring you to adjust filter time constants accordingly. The optimal ACQUITY UPLC flow rate differs from that of a typical HPLC column. The table below offers operating guidelines for ACQUITY UPLC columns under both isocratic and gradient conditions.

  • Page 25: Carryover

    Carryover • Keep the seal-wash line primed. • Prime solvent lines during system startup. • Keep all solvent lines primed. • Monitor the waste level, to ensure that it is never too high. • When starting the binary solvent manager, ensure solvent flows through pump A and pump B.

  • Page 26: Contamination

    HPLC/MS Systems (part number 715001307). Temperature Waters recommends you set sample temperature limits when configuring a method in the data application. Always ensure that your temperature limits are set in accordance with your analysis method. (Refer to online Help for instructions.)

  • Page 27: Preventing Leaks

    Preventing leaks The Load Ahead and Loop Offline options of the ACQUITY UPLC I-Class IVD SM-FTN help decrease cycle time. See the ACQUITY UPLC Console online Help for details on how to use these features. To prevent the loss of sample, note that the Load Ahead option...

  • Page 28: Sample Preparation

    2 Optimizing Performance Sample preparation UPLC analysis places some additional restrictions on sample preparation. Particulates The small column frit size (0.2 µm) can become blocked more easily than larger HPLC column frits (2.0 µm). Particle-free mobile phase solvents and sample solutions are therefore essential for UPLC analysis. See “General guidelines”...

  • Page 29: Preparing The System

    Starting the ACQUITY UPLC Console........... 38 Preparing system hardware Powering-on the system Powering-on the system entails starting the ACQUITY UPLC I-Class IVD system workstation, system modules, and MassLynx software. Each device or instrument beeps three times and runs a series of startup tests. See “Status...

  • Page 30: Monitoring System Module Leds

    3 Preparing the System To power-on the system: Power-on the ACQUITY UPLC I-Class IVD system’s workstation. Power-on the binary solvent manager and sample manager by pressing the power switch on the top, left-hand side of each devices’ door. “Status LEDs” on page 30 “Power LED”...

  • Page 31: Enabling The Leak Sensors

    Indicates a module’s failure that prevents its further operation. Power-off the module, and then power-on. If the LED is still steady red, contact your Waters service representative. Enabling the leak sensors When you power-on the system, the leak sensors default to disabled Rule: status unless previously enabled.

  • Page 32: Starting The System

    3 Preparing the System To enable the leak sensor for an individual module, click the status on the left-hand side of the module’s description. To enable all leak sensors, click Enable All. Tip: Starting the system Use the Start up system function after the system has been idle a short period of time (as much as 12 hours) and when you plan to use the same solvents that you used during the previous run.

  • Page 33: Configuring Masslynx Software

    Configuring MassLynx software Click OK. The system primes the selected solvents, primes the sample Result: manager with one weak-wash prime (using the wash and sample syringes), and ignites the lamp in the detector. Configuring MassLynx software Configure the MassLynx system software, for use with ACQUITY: •...
  • Page 34 3 Preparing the System The following table describes the items in the binary solvent manager control panel: Binary solvent manager control panel items: Control panel item Description Flow LED Displays the actual flow LED on the front panel of the binary solvent manager unless communications with the binary solvent manager are lost.
  • Page 35 ACQUITY control panels Additional functions in the binary solvent manager control panel: Control panel function Description Start up system Brings the system to operational conditions after an extended idle period or when switching to different solvents. See “Starting up the system” in the Binary Solvent Manager Operator’s Overview and Maintenance Information.

  • Page 36: Sample Manager Control Panel

    3 Preparing the System Sample manager control panel The sample manager control panel displays current sample compartment and column heater temperatures and set points. You can edit these values when the system is idle by clicking the underlined value. You cannot edit sample manager set points while the system is running samples.
  • Page 37 ACQUITY control panels Sample manager control panel items: (Continued) Control panel item Description Current Column Heater Displays the current column heater temperature to 0.1 °C resolution, even when Temperature active temperature control is disabled. Column Heater Set Point Displays the current column heater set point, to 0.1 °C resolution.

  • Page 38: Starting The Acquity Uplc Console

    3 Preparing the System Starting the ACQUITY UPLC Console The ACQUITY UPLC Console software provides a way to configure settings, monitor performance, run diagnostic tests, and maintain the system and its modules. It replaces the keypads and small-screen displays traditionally found on the fronts of system modules.

  • Page 39: Maintenance Procedures

    Maintenance schedule The following tables list periodic maintenance schedules that ensure optimum instrument performance of the ACQUITY UPLC I-Class IVD system. The maintenance frequencies shown apply to instruments that normally receive moderate use. When using the system throughout the day (and on nights and weekends), or when using aggressive solvents such as buffers, perform these maintenance tasks more frequently.
  • Page 40 4 Maintenance Procedures Column Manager maintenance procedures: Maintenance Frequency procedure Replace the leak As needed. For example, when the leak sensor is sensor on the column damaged or an error message states the leak sensor heater is not detected. Replace the column As needed.
  • Page 41 Maintenance schedule Column Manager maintenance procedures: (Continued) Maintenance Frequency procedure Replace the column Replace as part of the annual scheduled routine in-line filter frit maintenance and, thereafter, as needed. The typical failure modes are as follows: • High back pressure. The column in-line filter causes a high pressure shutdown.
  • Page 42 4 Maintenance Procedures Sample Manager Flow-Through-Needle maintenance procedures: Maintenance Frequency procedure Clean the instrument Perform as part of your annual routine scheduled with a soft, lint-free maintenance and when solvent or dust cloth, or paper accumulates. dampened with water Replace the leak As needed.
  • Page 43 Maintenance schedule Binary Solvent Manager maintenance procedures: Maintenance Frequency procedure Replace the leak As needed. For example, when the leak sensor is sensor damaged or an error message states the leak sensor is not detected. Replace the mixer Replace as part of the annual scheduled routine maintenance and, thereafter, as needed.
  • Page 44 4 Maintenance Procedures Binary Solvent Manager maintenance procedures: (Continued) Maintenance Frequency procedure Replace the in-line As needed. The typical failure modes are as follows: filter • High back pressure. The in-line filter causes a high pressure shutdown. In this case, disconnect the in-line filter.

  • Page 45: Spare Parts

    Spare parts To ensure that your system operates as designed, produces Warning: accurate results, and is not damaged, use only Waters Quality Parts®. Visit www.waters.com/wqp for information about Waters Quality Parts, including how to order them Listed below are parts you may want to keep on hand for repairs and maintenance: •...

  • Page 46: Troubleshooting With Connections Insight

    Connections INSIGHT is an “intelligent” device management (IDM) Web service that enables Waters to provide proactive service and support for the ACQUITY UPLC system. To use Connections INSIGHT, you must install its service agent software on your MassLynx workstation. In a client/server system, you must also install the service agent, on the computer from which you control the system.

  • Page 47: Safety And Handling

    Material Safety Data Sheets for the solvents in use). To avoid electric shock, do not remove the instrument’s panels. Warning: The panels cover components that are serviceable only by Waters technicians. To avoid electric shock, ensure that the instrument is in Warning: Standby mode before beginning any maintenance operation.

  • Page 48: Configuring Maintenance Warnings

    4 Maintenance Procedures • Instrument is in Standby mode. • LC flow is diverted to waste or set to off. • Desolvation gas is turned off. Appendix A for safety advisory information. Configuring maintenance warnings Maintenance counters provide real-time usage status information that can help you determine when to schedule routine maintenance for specific components.

  • Page 49: A Safety Advisories

    Heed all warnings when you install, repair, and operate Waters instruments. Waters assumes no liability for the failure of those who install, repair, or operate its instruments to comply with any safety precaution.
  • Page 50 A Safety Advisories When the following symbols appear in a manual’s narratives or procedures, their accompanying text identifies the specific risk and explains how to avoid (Risk of danger. When this symbol appears on an instrument, Warning: consult the instrument’s user documentation for important safety-related information before you use the instrument.) (Risk of hot surfaces.) Warning:...

  • Page 51: Specific Warnings

    The following warnings can appear in the user manuals of particular instruments and on labels affixed to them or their component parts. Burst warning This warning applies to Waters instruments fitted with nonmetallic tubing. Pressurized nonmetallic, or polymer, tubing can burst. Warning: Observe these precautions when working around such tubing: •...
  • Page 52 Biohazard warning This warning applies to Waters instruments that can be used to process material that can contain biohazards: substances that contain biological agents capable of producing harmful effects in humans.

  • Page 53: Caution Advisory

    To avoid damaging the instrument’s case, do not clean it with Caution: abrasives or solvents. Warnings that apply to all Waters instruments When operating this device, follow standard quality-control procedures and the equipment guidelines in this section. July 7, 2014, 715003736IVD Rev. C...
  • Page 54 A Safety Advisories Changes or modifications to this unit not expressly approved by the Attention: party responsible for compliance could void the user’s authority to operate the equipment. Toute modification sur cette unité n’ayant pas été expressément Important: approuvée par l’autorité responsable de la conformité à la réglementation peut annuler le droit de l’utilisateur à...
  • Page 55 Warnings that apply to all Waters instruments Use caution when working with any polymer tubing under pressure: Warning: • Always wear eye protection when near pressurized polymer tubing. • Extinguish all nearby flames. • Do not use tubing that has been severely stressed or kinked.
  • Page 56 A Safety Advisories fare attenzione quando si utilizzano tubi in materiale polimerico Attenzione: sotto pressione: • Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. • Spegnere tutte le fiamme vive nell'ambiente circostante. • Non utilizzare tubi eccessivamente logorati o piegati. •...
  • Page 57 Warnings that apply to all Waters instruments 警告： 当有压力的情况下使用管线时，小心注意以下几点： • 当接近有压力的聚合物管线时一定要戴防护眼镜。 • 熄灭附近所有的火焰。 • 不要使用已经被压瘪或严重弯曲的管线。 • 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸。 • 要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀，大大降低管线的耐 压能力。 경고 : 가압 폴리머 튜브로 작업할 경우에는 주의하십시오 . • 가압 폴리머 튜브 근처에서는 항상 보호 안경을 착용하십시오 . • 근처의 화기를 모두 끄십시오 .
  • Page 58 A Safety Advisories The user shall be made aware that if the equipment is used in a Warning: manner not specified by the manufacturer, the protection provided by the equipment may be impaired. L’utilisateur doit être informé que si le matériel est utilisé d’une Attention: façon non spécifiée par le fabricant, la protection assurée par le matériel risque d’être défectueuses.

  • Page 59: Electrical And Handling Symbols

    Electrical and handling symbols Electrical and handling symbols Electrical symbols These can appear in instrument user manuals and on the instrument’s front or rear panels. Electrical power on Electrical power off Standby Direct current Alternating current (single phase) Protective earth terminal Frame or chassis ground Fuse July 7, 2014, 715003736IVD Rev.

  • Page 60: Handling Symbols

    A Safety Advisories Handling symbols These handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments. Keep upright! Keep dry! Fragile! Use no hooks! July 7, 2014, 715003736IVD Rev. C...

  • Page 61: Physical Specifications

    These system specifications depend on the conditions in individual laboratories. Refer to the ACQUITY UPLC I-Class (IVD) System Site Preparation Guide (part number 715003724), or contact Waters Technical Service for details. For specifications on the mass spectrometer, see the Xevo TQ-S Note: IVD Mass Spectrometry System Operator’s Overview and Maintenance...

  • Page 62: B Specifications

    B Specifications Sample manager - FTN The following table lists the physical specifications for the ACQUITY UPLC I-Class IVD SM-FTN: Sample manager - FTN physical specifications: Attribute Specification Height 27.1 cm (10.7 inches) Width 34.3 cm (13.5 inches) Depth 71.2 cm (28.0 inches) Weight 26.1 kg (57.5 pounds) Column heater...

  • Page 63: Tq-S Mass Spectrometer

    The following table lists the environmental specifications for the ACQUITY UPLC I-Class IVD system: To prevent inaccurate results, do not operate the instrument Warning: outside the operating temperature and humidity specified. ACQUITY UPLC I-Class IVD system environmental specifications: Attribute Specification Acoustic noise <65 dBA, system: binary solvent...
  • Page 64 B Specifications The following table lists the electrical specifications for the ACQUITY UPLC I-Class IVD system: ACQUITY UPLC I-Class IVD system electrical specifications: Attribute Specification Class I Protection class Overvoltage category Pollution degree Normal (IPX0) Moisture protection Line voltages, nominal...

  • Page 65: Binary Solvent Manager Input/Output Specifications

    Environmental specifications Binary solvent manager input/output specifications The following table lists the input/output specifications for the ACQUITY UPLC I-Class IVD BSM: BSM input/output specifications: Attribute Specification Contact closure Maximum voltage: 30 VDC outputs (SW1 to SW3) Maximum current: 0.5 A Maximum VA rating: 10 W Contact resistance (nominal): 0.2 ohms Screw terminal connector...

  • Page 66: Sample Manager - Ftn Input/Output Specifications

    B Specifications BSM input/output specifications: (Continued) Attribute Specification Auxiliary input 1 TTL signal or contact closure: Input voltage range: ±30 VDC Logic High: ≥3.0 VDC Logic Low: ≤1.9 VDC Minimum pulse width: 100 msec Screw terminal connector Auxiliary input 2 TTL signal or contact closure: Input voltage range: ±30 VDC Logic High: ≥3.0 VDC...

  • Page 67: Xevo Tq-S Input/Output Specifications

    200-240 V, 50/60 Hz (two included) Wetted materials of construction Binary solvent manager The following table lists the wetted materials of construction for the ACQUITY UPLC I-Class IVD BSM: BSM wetted materials of construction: Description Specification Wetted materials 316L stainless steel, UHMWPE blend, MP35N, titanium alloy, gold, sapphire, ruby, zirconia, Nitronic 60, DLC, fluoropolymer, PEEK™...

  • Page 68: Column Heater

    B Specifications Column Heater The following table lists the wetted materials of construction for the ACQUITY UPLC I-Class IVD column heater: Wetted materials of construction: Description Specification Wetted materials 316L stainless steel July 7, 2014, 715003736IVD Rev. C...

  • Page 69: C External Connections

    External Connections A Waters technical service representative unpacks and installs your ACQUITY UPLC I-Class IVD system modules. To avoid damaging system modules, Contact Waters Caution: Technical Service (see page iv). If you must transport a system component or remove it from service, contact Waters Technical Service for recommended cleaning, flushing, and packaging procedures.
  • Page 70 C External Connections Solvent flow for ACQUITY UPLC I-Class IVD system with an SM-FTN: SM wash Solvent tray solvent BSM solvents Mass spectrometer* Divert Valve Valve Valve Column Column heater Sample Manager - FTN Sample syringe To waste Needle Injector...

  • Page 71: External Wiring Connections

    External wiring connections External wiring connections ACQUITY UPLC I-Class IVD instrument external wiring connections The rear panel connections for ACQUITY UPLC I-Class IVD system modules are shown below Solvent tray Drain tube outlet Column heater Xevo TQ-S External power and...

  • Page 72: Ethernet Connections

    The sample manager is connected internally to the Ethernet switch. To ensure optimum performance and signal integrity, use only Requirement: approved (or Waters supplied), shielded, CAT 5 ethernet cables. Ethernet Instrument Getting Started Guide, part number See also: 71500074403.

  • Page 73: Signal Connections

    Signal connections Signal connections Connecting signal cables Refer to the signal connection location shown on the silk-screened label affixed to the rear panel of each system module. Required materials • 9/32-inch nut driver • Flat-blade screwdriver • Connector • Signal cable To connect signal cables: Insert the connector into the connector port on the back of the system module.
  • Page 74 C External Connections Using the flat-blade screwdriver, attach the positive and negative leads of the signal cable to the connector. Screw Connector Signal cable Fit the grounding cable’s fork terminal on the rear panel grounding stud, and secure the terminal with the locking nut. Use the 9/32-inch nut driver to tighten the locking nut until the fork Tip: terminal does not move.
  • Page 75 Signal connections Binary solvent manager I/O signal connectors The rear panel of the binary solvent manager includes two removable connectors that hold the screw terminals for I/O signal cables. These connectors are keyed so that they can be inserted only one way. Binary solvent manager I/O signal connectors: Connector I Connector II...
  • Page 76 C External Connections Binary solvent manager analog-out/event-in connections: (Continued) Signal connection Description 0−2V Analog 1 Out Outputs the analog signal to a device such as an integrator or strip-chart recorder. Via the MassLynx software, you can select one of the following signals as the chart out signal: •...
  • Page 77 Signal connections Chart-out signal conditions: Parameter Setting at 0 Parameter Setting at Signal Volts (Minimum) 2.000 Volts (Maximum) Flow Rate 0.000 mL/min 2 mL/min System Pressure –345 kPa (–3.45 bar, 124,106 kPa (1241 bar, –50 psi) 18,000 psi) Composition 0.0% 100.0% Sample manager I/O signal connectors The rear panel of the sample manager includes a removable connector that...

  • Page 78: Connecting To The Electricity Source

    Recommendation: (UPS) for optimum, long-term, input voltage stability. For sizing and selection of the UPS or line conditioner, contact Waters technical support. Connect the female end of the power cord to the receptacle on the rear panel of each module.
  • Page 79 Connecting to the electricity source FlexCart power connections (optional): To circuit A AC line Network switch (if needed) Sample manager Binary solvent manager FlexCart power strips LCD/monitor Computer workstation TP02578 To circuit B AC line All cord lengths are 1 meter long except the LCD/ monitor cord, which is 2 meters long.
  • Page 80 C External Connections July 7, 2014, 715003736IVD Rev. C...

  • Page 81: D Solvent Considerations

    Solvent guidelines Solvent Considerations This information applies to the ACQUITY UPLC I-Class IVD system modules. To avoid injury, familiarize yourself with materials Warning: and their hazards, observe Good Laboratory Practice (GLP), and consult your organization’s safety representative regarding proper use and handling.

  • Page 82: Solvent Quality

    Store mobile phases in borosilicate glass reservoirs type 1, class A or type . Use brown-stained glassware to inhibit microbial growth. Use aluminum foil or Waters caps to cover the reservoirs. Water Use water only from a high-quality water purification system. If the water system does not deliver filtered water, filter the water through a 0.2-µm...

  • Page 83: Solvent Recommendations

    ACQUITY UPLC BEH column chemistries. Waters evaluated the system’s reliability using traditional reversed-phase solvents. This section lists solvents recommended for the ACQUITY UPLC I-Class IVD system. Contact Waters Customer Service to determine whether you can use solvents that do not appear in the list without adversely affecting instrument or system performance.

  • Page 84: Solvents To Avoid

    D Solvent Considerations Solvents to avoid Avoid these solvents: • Solvents that contain halogens: chlorine, fluorine, bromine, or iodine. • Strong acids. (Use them only in weak concentration, less than 5%, unless using them as cleaning agents. Avoid using acids as mobile phases when their pH is less than 2.0.) •...

  • Page 85: Recommended Sample Diluents

    Solvent recommendations Recommended sample diluents • Acetonitrile • Acetonitrile/water mixtures • Chloroform • Dimethylformamide (DMF) • Dimethyl sulfoxide (DMSO) • Isooctane • Isopropanol • Methanol • Methanol/water mixtures • Methylene chloride • Water Recommended cleaning agents You may use these cleaning agents: •...

  • Page 86: System Recommendations

    • Contact Waters for recommended system cleaning and flushing procedures. • Contact your Waters sales representative or local technical support organization to determine the typical chromatography conditions and/or instrument operating parameters suitable for the ACQUITY I-Class IVD system. •...

  • Page 87: Sample Manager Recommendations

    Properties of common solvents Sample manager recommendations • Typical organic sample diluents such as dimethylsulfoxide (DMSO) and dimethylformamide (DMF) are supported. • Do not use buffers as needle wash solvents. You can use acids and bases. Properties of common solvents The following table lists the properties for some common chromatographic solvents: Properties of common solvents:...

  • Page 88: Solvent Miscibility

    D Solvent Considerations Properties of common solvents: (Continued) Vapor Pressure Boiling Flash Solvent mm Hg (Torr) Point (°C) Point (°C) Isobutyl alcohol 8.8 at 20 °C 107.7 Isopropyl alcohol 32.4 at 20 °C 82.26 Isopropyl myristate <1 at 20 °C 192.6 Methanol 97 at 20 °C...

  • Page 89: Using Miscibility Numbers (M-Numbers)

    Solvent miscibility • Temperature affects solvent miscibility. If you are running a high-temperature application, consider the effect of the higher temperature on solvent solubility. • Buffers dissolved in water can precipitate when mixed with organic solvents. Solvent miscibility: Viscosity Boiling Miscibility λ...

  • Page 90: Solvent Viscosity

    D Solvent Considerations • When the difference is 17 or greater, the liquids are immiscible, or their critical solution temperature is above 75 °C. Some solvents prove immiscible with solvents at both ends of the lipophilicity scale. These solvents receive a dual M-number: •...

  • Page 91: E Flexcart Assembly

    Because the assembled system weighs at least 140.6 kg, Recommendation: Waters recommends that you assemble the instruments and components on the FlexCart. To assemble the FlexCart Remove the contents from the box packaged in the FlexCart.
  • Page 92 E FlexCart Assembly FlexCart components: Monitor mounting Top tray bracket Power Shelf mounting connectors (2) holes Lock knobs (2) Height adjusting screw TORX screws Remove the 4 Phillips screws from the support plate at the rear of the monitor, and mount the monitor onto the movable arm assembly. Refer to the IBM instruction book, if necessary.

  • Page 93: Adjusting The Flexcart's Height

    Adjusting the FlexCart’s height Loosen the 2 T25 TORX screws, at the bottom left-hand side of the cart, that secure the bracket for the CPU. Attach the power and video cables to the CPU, and place it in position on the bottom shelf of the cart.

  • Page 94: Locking The Flexcart In Place

    E FlexCart Assembly Insert the crank handle into the bottom front of the cart, and turn it to raise or lower the cart. Crank handle If your system includes a mass spectrometer and it is positioned on Tip: the right-hand side of the system stack, set the cart to a height that minimizes the length of tubing needed between the instruments stacked on the cart and the mass spectrometer.

  • Page 95: Moving The Assembled Flexcart

    Moving the assembled FlexCart To release the FlexCart floor lock brake: Release the cart by depressing the brake release bar located at the front of the cart. Release bar Lock pedal Moving the assembled FlexCart To avoid spills of damaging solvent, remove all solvent Caution: reservoirs from the solvent tray before moving the cart.
  • Page 96 E FlexCart Assembly July 7, 2014, 715003736IVD Rev. C...

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