Waters ACQUITY APC XT 45 Care And Use Manual

[ CARE AND USE MANUAL ]
ACQUITY APC Columns
CONTENTS
I. Introduction
II. Getting Started
a. Preparing the System
b. Column Connections
c. Column Installations
d. eCord Installation
e. Column Equilibration
III. Column Use
a. Guidelines
b. Calibration
c. Useable Flow-Rate Ranges
d. Minimizing Band Spread
IV. Troubleshooting
V. COLUMN CLEANING, REGENERATION
AND STORAGE
a. Cleaning and Regeneration
b. Storage
VI. Ecord Intelligent Chip Technology
a. Introduction
b. Installation
c. Column Use Information

I. INTRODUCTION

Thank you for choosing an ACQUITY APC™ Column. The
ACQUITY APC Columns are designed to achieve maximum
separation performance when used with the ACQUITY™
Advanced Polymer Chromatography™ (APC™) System. Please
take a few moments to read this manual carefully. Following
the recommendations in this manual will prolong column life
and enhance chromatographic reproducibility.
ACQUITY APC Columns are packed with sub-3-µm ethylene
bridged hybrid (BEH) particles that provide mechanical
strength, packed-bed stability and high separation efficiency.
They deliver superior chromatographic performance for all
polymer classes, including low molecular weight aqueous
and organic soluble polymers, up to a molecular weight of
2,000,000 g/mole (Table 1).
To maximize separation selectivity and performance
ACQUITY APC Columns are available in two bonding
technologies optimized for each solvent class:
ACQUITY APC XT: Packed with a high-coverage trimethyl
silane bonded to a BEH substrate. These columns are
recommended for extended temperature (< 90 °C)
separations in organic solvents.
ACQUITY APC AQ: Feature unbonded BEH substrate.
Recommended for room temperature (< 45 °C) separations
in aqueous solvents.
ACQUITY APC Columns are manufactured in an ISO 9001
facility and are held to narrow specification ranges to ensure
reproducible performance. Every column is individually tested
and a Certificate of Batch Analysis is provided on the attached
eCord™ Intelligent Chip.
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