Waters ACQUITY UPLC Care And Use Manual
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Waters ACQUITY UPLC Care And Use Manual

Oligonucleotide beh c18 column
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[ CARE AND USE MANUAL ]
ACQUITY UPLC and ACQUITY Premier Oligonucleotide BEH C
CONTENTS
I.
INTRODUCTION
II.
d. Procedure for Using New,
Out-of-Box Columns
III. COLUMN USE
a. Sample Preparation
g. Mixer Options
IV.
COLUMN CLEANING, REGENERATION,
AND STORAGE
a. Cleaning and Regeneration
V.
b. Installation
VI.
VII.

I. INTRODUCTION

Thank you for choosing a Waters™ ACQUITY™ UPLC™ and/or
ACQUITY Premier Oligonucleotide BEH C
specifically for use on ACQUITY UPLC and ACQUITY Premier
Systems. The separation of detritylated oligonucleotides on
Waters second generation of hybrid-silica BEH Technology™
particles are based on the well established method on
ion-pair, reversed-phase chromatography. ACQUITY UPLC
and ACQUITY Premier Oligonucleotide BEH C
are available in several configurations to address different
application needs. The ACQUITY UPLC and ACQUITY Premier
Oligonucleotide BEH C
packing material is manufactured in
18
a cGMP, ISO 9002 certified plant using ultra pure reagents.
Each batch of ACQUITY UPLC and ACQUITY Premier
Oligonucleotide BEH C
material is chromatographically tested
18
with acidic, basic, and neutral analytes and the results are held
to narrow specification ranges to assure excellent, reproducible
performance. In addition, every column is individually tested
and the associated Performance Test Chromatogram and
Certificate of Acceptance information is available through the
attached eCord Intelligent Chip Technology.
Note: Optimum performance of ACQUITY UPLC and ACQUITY
Premier Oligonucleotide BEH C
an appropriately configured Waters ACQUITY UPLC Systems
(e.g., See Section II, g). Consequently, use of ACQUITY UPLC
and ACQUITY Premier Oligonucleotide BEH C
conventional HPLC systems is not recommended.
Columns
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Column designed
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Columns
18
Columns is best assured using
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Columns on
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1

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Summary of Contents for Waters ACQUITY UPLC

  • Page 1 Columns is best assured using AND STORAGE an appropriately configured Waters ACQUITY UPLC Systems a. Cleaning and Regeneration (e.g., See Section II, g). Consequently, use of ACQUITY UPLC b. Storage and ACQUITY Premier Oligonucleotide BEH C Columns on conventional HPLC systems is not recommended.
  • Page 2 Optimized column inlet tubing (p/n: 430001084) is supplied with the ACQUITY UPLC System. The inject valve end of the tubing d. Procedure for Using New, Out-of-Box Columns is clearly marked with a blue shrink tube marker. Insert the...
  • Page 3 40 to 59.5% B in 26 minutes (8–11.9% acetonitrile, 0.15% acetonitrile per minute) Detection: 260 nm, 5 scans per second Figure 1. Separation using the MassPREP Oligonucleotide Standard on an ACQUITY UPLC Oligonucleotide BEH C Column . ACQUITY UPLC and ACQUITY Premier Oligonucleotide BEH C Columns...
  • Page 4 2. Add 41.56 mL (67.17 g) of HFIP into 956.36 g of water and mix well. 3. Slowly add 2.08 mL (1.52 g) of TEA. 4. The pH of final buffer is approximately 7.9 +/- 0.1. ACQUITY UPLC and ACQUITY Premier Oligonucleotide BEH C Columns...
  • Page 5 0.20 µm membrane filtered, LC grade water is the recommended The recommended flow rate for oligonucleotide separations weak wash solvent solution for all ACQUITY oligonucleotide performed on a 2.1 x 50 mm ACQUITY UPLC and ACQUITY separation methods. Premier Oligonucleotide BEH C Column is 0.2 mL/min.
  • Page 6 ACQUITY UPLC and ACQUITY Premier Oligonucleotide BEH C Columns...
  • Page 7 Waters, The Science of What’s Possible, ACQUITY, UPLC, BEH Technology, MaxPeak, eCord, Empower, Milford, MA 01757 U.S.A. MassLynx, and MassPREP are trademarks of Waters Corporation. PEEK is a trademark of Victrex plc. T: 1 508 478 2000 All other trademarks are the property of their respective owners.

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