Waters Autospec Premier Operator's Manual page 105

6. In the Channel Time (ms) text box, set the time for which this mass is to
be monitored, typically 50 to 150 ms.
7. In the Delay Time (ms) text box, set the time delay between finishing
monitoring the previous mass and starting monitoring this mass (in the
MRM Masses list).
This is a minimum of 20ms and varies depending on the magnet
Tip:
mass transition (the automatically calculated magnet mass position to
transmit the specific product) from one monitored reaction to the next.
The delay allows the instrument's electronics and magnet to stabilize
between monitoring successive masses. Use the MRM function editor
auto-delay function to set these times and, if necessary, adjust them
according to the specific procedure provided by the service engineer who
installed your instrument.
8. Click OK.
The details entered in the MRM Mass dialog box appear in the
Result:
MRM Masses list.
9. Repeat steps 3 to
monitored by the function.
10. Enter an appropriate value, for example 6 minutes, in the Acquisition
Parameters pane Start Time (min) text box.
Start the first function in a function list before the sample has
Tip:
started eluting from the column. Set the start time to take account of the
solvent front and the retention time of the first eluting analyte.
8, as required, to enter the remaining masses to be
Example of MRM experiment set up 3-41