Waters Anionic Polar Pesticide Column Care And Use Manual

[ CARE AND USE MANUAL ]
Anionic Polar Pesticide Column
CONTENTS
I. INTRODUCTION
II. GETTING STARTED
a. VanGuard™ (Pre-columns)
b. Column Installation (with or without
VanGuard Pre-columns)
c. eCord™ Installation
d. Column Conditioning and Equilibration
E. USEFUL FUNCTIONAL TESTS FOR
BENCHMARKING A NEW COLUMN
III. COLUMN USE
a. Sample Preparation
b. Solvents
c. Additives
d. Pressure
e. Temperature
f. pH
g. Flow rate
IV. TROUBLESHOOTING
V. CLEANING, REGENERATION,
AND STORAGE
a. Cleaning
b. Storage
I. INTRODUCTION
Thank you for choosing Waters™ Anionic Polar Pesticide
Column. This column enables scientists to analyze and
separate highly anionic polar pesticides on HPLC, UHPLC, or
UPLC™ instrumentation. This column is specifically designed
for excellent peak shape, with added selectivity for analysis
of various polar pesticides such as glyphosate operating
under HILIC/WAX conditions. This column is based on new
bonding technology used to create an adaptive selectivity
platform. Starting with the ethylene bridged hybrid (BEH)
particle, the patent pending bonding consists of a two-stage
functionalization of chromatographic particles. The first step
of the process controls retention characteristics, while the
second provides the unique selectivity of the phase.
Column:
Stationary phase:
Particle shape:
Particle size:
Pore size:
Pore volume:
Surface area:
Endcapped:
The packing materials used for the Anionic Polar Pesticide
Column are optimized for use with the ACQUITY™ UPLC
Systems. The Anionic Polar Pesticide Column is manufactured
in an ISO-certified plant using ultra-pure reagents. Each
batch of particles are tested, and the results are held to
narrow specification ranges to assure excellent, reproducible
performance. A Performance Chromatogram and Certificate
of Batch Analysis is provided, and the information is included
on the eCord Intelligent Chip. With the column part number
and lot number, this same column performance data and
batch information can be found on the Waters' website at
www.waters.com/coa.
* US Patent # 7,399,410, UK Patent # GB2408469.
Anionic polar pesticide
Diethylamine
Spherical
5 µm
130 Å
0.7 cc/g
185 m /g
2
Proprietary
1