Glow Luminescence - tecan infinite 200 Instructions For Use Manual

8. Quality Control
8.3.5

Glow Luminescence

Sensitivity
Measurement Parameters:
Plate Layout:
1 2
A Bx ATP
B Bx ATP
C Bx ATP
D Bx ATP
E
F
G
H
Material/Reagents:
140
®
For the with the option 'Luminescence the following tests may be
infinite 200
performed to prove the specifications:
•
Sensitivity
Perform the following measurement to determine the Sensitivity:
Parameter
Reading Mode
Integration Time
Settle Time
Plate Type
Part of the Plate
Pipette 200 µl of the ATP reagents into the appropriate wells according to the
plate layout:
3 4 5
Bx B B
Bx B B
Bx B B
Bx B B
-8
ATP 2*10 M ATP (final concentration in well)
B Blank (ATP reagent: Tris-EDTA=1:5)
Bx Blank (wells used for cross-talk calculation)
BioThema ATP Kit (ATP-Kit SL 144-041, BioThema AB)
1 Greiner 96-well plate white
200 µl Pipette + tips
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
Setting
Luminescence
1000 ms
0
GRE96fw
A1 – D10
6 7 8
B B B
B B B
B B B
B B B
9 10 11
B B
B B
B B
B B
12
2008-07