tecan infinite 200 Instructions For Use Manual page 130

8. Quality Control
Plate Layout:
<> 1 2
A
B
C
D
E
F
G
H
Material/Reagents:
Calculation of Detection Limit (Sensitivity):
130
Pipette 200 µl of 1 nm Fluorescein or the blank solution (0.01 M NaOH) into the
appropriate wells according to the plate layout:
3 4 5
1 nm Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377)
0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045)
1 Greiner 96-well plate black
200 µl Pipette + tips
Concentrat
=
DetectionL imit
(
mean
Concentration Concentration of the fluorophore in pM units
F
mean Average RFU value of wells filled with fluorophore
F
mean Average RFU value of wells filled with blank
B
stdev Standard deviation of RFU values of wells filled with blank
B
The result of the formula 'Detection Limit' determines the sensitivity in pM units.
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
6 7 8
ion
F
* 3 * Stdev
)
−
mean
F B
9 10 11
B
2008-07
12