tecan infinite 200 Instructions For Use Manual page 46

2. General Description
Example 4: Measurement of Ca
Plate
Part of the plate
Well
Kinetic Cycle
Kinetic condition
Injection
Fluorescence Intensity (1)
Fluorescence intensity (2)
Move Plate
46
2+
sensitive probes – Indo-1
Select an appropriate plate type. For fluorescence
measurements, black microplates are recommended.
For this example, a black 96 well plate was selected.
(Not shown); can be optionally selected if only part of the
plate should be processed
Mandatory for measurements with 'injection'
Select the number of necessary cycles
This strip allows actions to be performed once in a kinetic
run at a certain cycle. The intended injection strip below it
is only processed once at the selected cycle.
Injector A injects 20 µl with speed 200 µl/sec., refill mode:
not selectable; injection is performed at cycle 5 (defined
by kinetic condition strip)
Select the appropriate parameters for the first label:
Excitation wavelength: 340 nm, Emission wavelength:
410 nm; number of flashes: 25; integration time: 40; gain:
manual
Select the appropriate parameters for the second label:
Excitation wavelength: 340 nm, Emission wavelength:
480 nm; number of flashes: 25; integration time: 40; gain:
manual
Plate is moved out after finishing all wells
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 2008-07