Measurement Accessories - tecan infinite 200 Instructions For Use Manual

6.3.3

Measurement Accessories

Recommended Filters (infinite
Recommended Types of Microplates
Plate Definition File
(*.pdfx)
GRE6ft
GRE12ft
GRE24ft
GRE48ft
GRE96ft
GRE96fb_chimney
GRE96fw_chimney
2008-07
®
F200 Only)
Please ask your local Tecan dealer for a recommended filter set. Filters designed
for a different type of instrument will not necessarily perform well with the
®
infinite F200.
If the excitation and the emission maximum of a fluorescent species are
close together, they should not be directly translated into center
wavelengths for fluorescence filters.
To provide acceptable background, usually, the upper cutoff for excitation
wavelengths on the one hand, and the lower cutoff for emission wavelengths on
the other hand need to be separated. This compromise depends on the blocking
properties of the filters. For many fluorescent molecules signal may be improved
by expanding filter bandwidth away from the other band pass, respectively.
Generally, for high fluorescence sensitivity, black microplates are recommended.
For low concentrations of TRF labels, white microplates seem superior. You may
check if white plates are superior with UV excitation wavelengths.
We do not recommend using volumes less than a third of the maximum volume.
When using lower volumes, check the availability of a suitable plate type.
In order to ensure good performance for Fluorescence Bottom Reading, we
recommend using black plates with transparent bottom.
All standard microplates from 6 to 384 wells (maximum plate height 23 mm
including lid) that conform to the following standards can be measured:
ANSI/SBS 1-2004, ANSI/SBS 2-2004; ANSI/SBS 3-2004 and ANSI/SBS 4-2004.
When installing the operating software (i-Control or Magellan), pre-defined plate
definition files are installed. Please refer to the following list for the corresponding
ordering numbers of the microplates. Please order microplates at your local
microplate supplier.
Catalog Number
655 077 (Fluotrac 600)
655 076 (Fluotrac 200)
655 074 (Lumitrac 600)
655 075 (Lumitrac 200)
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
6. Operating the infinite® 200
Note
657 160
657 185
665 180
665 102
662 160
662 102
677 180
677 102
655 101
655 161
655 079
655 086
655 073
655 083
Manufacturer
Greiner Bio-One,
www.gbo.com/bioscience
Greiner Bio-One,
www.gbo.com/bioscience
Greiner Bio-One,
www.gbo.com/bioscience
Greiner Bio-One,
www.gbo.com/bioscience
Greiner Bio-One,
www.gbo.com/bioscience
Greiner Bio-One,
www.gbo.com/bioscience
Greiner Bio-One,
www.gbo.com/bioscience
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