Fluorescence Top/Bottom Optics - tecan infinite 200 Instructions For Use Manual

5.1.2

Fluorescence Top/Bottom Optics

Fluorescence Intensity Lens System Top
Excitation Spot Size
Fluorescence Fiber Bundle Top and Bottom
Fluorescence Bottom Mirror
2008-07
Flash light enters the optical system being focused by the condenser onto the
entrance slit of the Excitation Monochromator. The wavelength of the excitation
light is selected within the monochromator. After passing the monochromator, the
excitation light is coupled into a fiber bundle guiding the light to the top or bottom
measuring head. The light is then focused into the sample by the top/bottom lens
system.
The fluorescence light is collected by the top/bottom lens system again, coupled
into the fluorescence fiber bundles and guided to the detection system.
The Fluorescence Measuring Optics Top is built from the following components:
•
Fluorescence Intensity Lens System Top
•
Fluorescence Fiber Bundle
The bottom optics consists of the following components:
•
Fluorescence Bottom Mirror
•
Fluorescence Fiber Bundle
The exit side of the bundle acts as a color specific light source. The lens system
at the end of the excitation top fiber is designed to focus the excitation light into
the sample, and also collect the fluorescence light and focus it back onto the
fluorescence fiber bundle.
The objective lenses are made from fused silica. This material provides high UV
transmission and is virtually void of auto-fluorescence.
The size of the fiber bundle cross section determines the diameter of the beam
waist (spot size) in the microplate well. The spot diameter for the M-series is
about 3 mm for the top optics and 2 mm for the bottom optics.
The fiber bundle plugged into the top/bottom measuring head contains a
homogeneous mixture of both excitation and emission fibers. The emission fibers
guide the fluorescence light emission monochromator head where a lens system
focus the light onto the entrance slit of the Emission Monochromator.
The exit side of the bundle acts as a color specific light source. The mirror at the
end of the excitation bottom fiber is designed to focus the excitation light into the
sample and also collects the fluorescence light and focuses it back onto the
fluorescence fiber bundle.
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
5. Optical System
69