tecan infinite 200 Instructions For Use Manual page 137

Material/Reagents:
Calculation of Detection Limit (Sensitivity):
Precision
Measurement Parameters:
Plate Layout:
Calculation of Precision:
2008-07
1 nm Europium (B119-100, HVD Live Sciences)
Enhancement Solution (=Blank) (1244-105, HVD Live Sciences)
1 Greiner 96-well plate white
200 µl Pipette + tips
See 8.3.1 Fluorescence Top: Sensitivity.
Perform the following measurement to determine the precision/reproducibility:
Parameter
Reading Mode
Exc Wavelength
Em Wavelength
Number of flashes
Integration Time
Lag Time
Time between Move and Flash
Gain
Plate Type
Part of the plate
Kinetic
Interval Time
See 8.3.3 Time Resolved Fluorescence Precision.
See 8.3.1 Fluorescence Top: Precision.
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
8. Quality Control
Setting
Fluorescence Top
®
: 340 nm (35)
infinite F200
®
: 340 nm
infinite M200
®
: 612 nm (10 nm)
infinite F200
®
: 617 nm
infinite M200
25
400
100
0
Optimal
GRE96fw
A1
20 Cycles
Minimal
137