Fi Ratio Mode - tecan infinite 200 Instructions For Use Manual

6. Operating the infinite® 200
6.3.2

FI Ratio Mode

Ratio Mode
Filter Switch Time (infinite
98
Up to 4 labels may be measured well-wise. This measurement mode is called
'ratio mode'. Be aware that no 'ratio' calculation is performed after this
measurement. The Excel
have to be performed by the user.
®
F200)/Wavelength Switch Time (infinite
®
The can switch between two filters within 250 ms in case that the
infinite F200
selected labels are measured with the same gain. Otherwise, the switching time
is 400 ms. In this case the high voltage level at the PMT needs to be changed.
The high voltage applied to the PMT needs some time to stabilize.
®
The can switch between two wavelengths within 150 ms in case
infinite M200
that the selected labels are measured with the same gain and no order sorting
(OS) switching point is involved (see Table 6.3-1:for switching points). Otherwise,
the switching time is 400 ms. In this case the high voltage level at the PMT needs
to be changed. The high voltage applied to the PMT needs some time to stabilize.
The OS filter wheel needs to be moved.
Table 6.3-1: OSF (Order Sorting Filter) Switching Points (infinite
OSF Switching Point 1
OSF Switching Point 2
OSF Switching Point 3
Example:
Fura-2: This application involves a filter/wavelength switch between 340 and
380 nm on the excitation side. The emission is measured at about 510 nm. The
excitation filter/wavelength switch does not include an OS switch, therefore the
switch is possible within 150 ms on an infinite
®
infinite F200.
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
®
result sheet shows the raw data. Further calculations
Excitation Wavelength
316 nm
386 nm
561 nm
®
M200 and 250 ms on a
®
M200)
®
M200)
Emission Wavelength
401 nm
621 nm
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2008-07