I-Control And The Cuvette Port - tecan infinite 200 Instructions For Use Manual

5. Optical System
5.5.5

i-Control and the Cuvette Port

82
Cuvette Strip
For performing cuvette measurements, a 'Cuvette' strip is necessary (see Figure
5-10).
Figure 5-10: Cuvette strip
For a few applications it might be necessary to combine a microplate
measurement with a cuvette measurement. The i-Control software therefore
allows the usage of one cuvette strip and one plate strip within one measurement
script. The cuvette measurement has to be positioned before the microplate
measurement. To perform an accurate microplate measurement, the cuvette door
must not be open. The software therefore does not allow the user to use a 'Move
cuvette OUT' strip before the microplate measurement (see also chapter 5.5.6)
Cuvette Movements
The cuvette can be moved in and out with the 'cuvette in' and 'cuvette out'
buttons (see Figure 5-11) or by selecting Cuvette in/Cuvette out in the
Instrument/Movements dialog box.
Figure 5-11: Cuvette 'out' and 'in' button
Blanking
The software allows a so-called 'Blanking' measurement. 'Blanking' in the
Instrument menu is only available when a measurement script containing a
cuvette measurement is open. When Blanking is selected in the Instrument
menu, an absorbance measurement with the cuvette port is activated according
to the parameters (wavelength, flash number, settle time) of the active script. The
user is requested to insert the blank cuvette (e.g. containing buffer solution) and
to start the measurement. The blank data are then written into an Excel
spreadsheet. The data are also stored in the software and can be applied to the
following cuvette measurements performed with the same parameters. The blank
data are automatically subtracted when the check box Apply Blanking is
selected on the 'Absorbance' or 'Absorbance Scan' strip.
The blank data are stored in the software as long as no other blanking
measurement is performed or the software is closed. Be aware that the stored
blanking data will be overwritten without a warning message if another blanking
measurement is started. The stored blanking data will also be deleted without a
warning message when closing the software.
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
®
2008-07