Fp Measurements; Fluorescence Polarization; Measurement Blank Range - tecan infinite 200 Instructions For Use Manual

6. Operating the infinite® 200

6.4 FP Measurements

6.4.1

Fluorescence Polarization

6.4.2

Measurement Blank Range

102
Fluorescence Polarization (FP, P) is defined by the following equation:
( )
−
I I
⊥
= ||
P
( )
+
I I
⊥
||
where I and I equal the emission intensity of the polarized light parallel and
⎟⎟ ⊥
perpendicular to the plane of excitation, respectively. Polarization is a
dimensionless unit, generally expressed in mP units.
To start an FP measurement, the program strip must contain a valid
measurement Blank range and valid G-Factor settings.
Measurement blank reduction is performed automatically at each fluorescence
polarization measurement; the mean value of the respective blank wells will be
subtracted from each sample value (see 6.4.8).
In the Measurement group box, select the Blank range by clicking Change and
then selecting the wells filled with the measurement (sample) blank.
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 2008-07