Luminescence Optics - tecan infinite 200 Instructions For Use Manual

5. Optical System
5.7.1

Luminescence Optics

Fiber
Filter Wheel
90
In luminescence measurement mode, the
position and a moveable luminescence measurement head (see Figure 5-13:
Optical System Luminescence). The plate thickness is defined by selecting the
corresponding plate type in the software (see i-Control Instructions for Use).
A glass fiber guides the light from the sample to the detection unit. The fiber is
designed to measure 96-well plates as well as 384-well plates.
A filter wheel with 6 filter positions in front of the PMT window is switched to the
required luminescence channel. The sensitivity of the detection system makes it
necessary to attenuate high luminescence light levels; therefore, the filter wheel
can also switch a neutral density filter across the selected fiber exit.
Filter Wheel Position
Position 1
Position 2
Position 3
Position 4
Position 5
Position 6
* recommended for BRET ™2 and ChromaGlo™-Luciferase Assay
** recommended for BRET™ 1
See Figure 5-14 to Figure 5-17 for transmission spectra of luminescence filters.
100
90
80
70
60
50
40
30
20
10
0
400
Figure 5-14: Transmission spectrum of filter 'Lumi Magenta'
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
®
infinite
Filter
Lumi Green*
Lumi Magenta*
1 OD neutral density filter
No attenuation
Blue 1**
Green 1**
450 500
Wavelength [nm]
uses fixed microplate
200
550 600 650
2008-07