Time Resolved Fluorescence - tecan infinite 200 Instructions For Use Manual

8. Quality Control
8.3.3

Time Resolved Fluorescence

Sensitivity
Measurement Parameters:
Plate Layout:
136
®
For the with the option 'Fluorescence Top' the following tests may
infinite 200
be performed to prove the specifications:
•
Sensitivity
•
Precision/Repeatability
These test instructions are valid for the
®
•
infinite F200
•
Spectrally enhanced version
Perform the following measurement to determine the sensitivity:
Parameter
Reading Mode
Exc Wavelength
Em Wavelength
Number of flashes
Integration Time
Lag Time
Settle Time
Gain
Plate Type
Pipette 200 µl of 1 nm Europium solution or the blank solution (enhancement
solution) into the appropriate wells according to the plate layout:
<> 1 2
A
B
C
D
E
F
G
H
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
®
infinite 200
Setting
Fluorescence Top
®
infinite F200
®
infinite M200
®
infinite F200
®
infinite M200
25
400
100
0
Optimal
GRE96fw
3 4 5
:
: 340 nm (35)
: 340 nm
: 612 nm (10 nm)
: 617 nm
...
2008-07