Fluorescence Bottom - tecan infinite 200 Instructions For Use Manual

8.3.2

Fluorescence Bottom

Sensitivity
Measurement Parameters:
Plate Layout:
<> 1 2
A
B
C
D
E
F
G
H
2008-07
®
For the with the option 'Fluorescence Bottom' the following tests
infinite 200
may be performed to prove the specifications:
•
Sensitivity
•
Uniformity
•
Precision/Repeatability
These test instructions are valid for the
®
•
infinite F200
®
•
infinite M200
•
Standard version
•
Spectrally enhanced version
Perform the following measurement to determine the detection limit for
Fluorescein:
Parameter
Reading Mode
Exc Wavelength
Em Wavelength
Number of flashes
Integration Time
Settle Time
Gain
Plate Type
3 4 5
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
®
infinite 200
Setting
Fluorescence Bottom
®
infinite F200
®
infinite M200
®
infinite F200
®
infinite M200
25
40
0
Optimal
GRE96fb
6 7 8
8. Quality Control
:
: 485 (20 nm)
: 485 nm
: 535 (25 nm)
: 535 nm
9 10 11 12
133