tecan infinite 200 Instructions For Use Manual page 7

6.3.1
6.3.2
6.3.3
6.4
6.4.1
6.4.2
6.4.3
6.4.4
6.4.5
6.4.6
6.4.7
6.4.8
6.5
6.5.1
6.5.2
6.6
6.6.1
6.6.2
6.6.3
6.6.4
6.6.5
6.7
6.7.1
6.7.2
7. Instrument Features ................................................................ 115
7.1
7.2
7.3
7.3.1
7.3.2
7.3.3
7.3.4
7.4
7.5
7.6
7.6.1
7.7
7.8
7.9
7.10
7.11
2008-07
Instrument Parameters ..........................................................96
FI Ratio Mode ........................................................................98
Measurement Accessories ....................................................99
FP Measurements................................................................102
Fluorescence Polarization ...................................................102
Measurement Blank Range .................................................102
G-Factor Settings.................................................................103
Measurement with an Uncalibrated G-Factor ...................... 103
Measurement with a Simultaneous G-Factor Calibration..... 104
Measurement with a Calibrated G-Factor ............................ 105
Measurement with a Manual G-Factor................................. 106
Calculation of Fluorescence Polarization Parameters ......... 107
Optimize Absorbance Measurements................................ 108
Measurement Parameters ...................................................108
Absorbance Ratio Mode ...................................................... 108
Multiple Reads Per Well ...................................................... 109
MRW Type...........................................................................109
MRW Size............................................................................110
MRW Border ........................................................................110
Result Display in MS Excel
Miscellaneous Software Features of MRW.......................... 112
Optimize Luminescence Measurements............................ 113
Integration Time...................................................................113
Light Level Attenuation ........................................................113
Introduction..........................................................................115
Instrument Specifications................................................... 116
Fluorescence Intensity and
Time Resolved Fluorescence (TRF) ................................... 117
Definition of the Detection Limit ........................................... 118
Fluorescein (Fluorescence Intensity) Top............................ 118
Fluorescein (Fluorescence Intensity) Bottom....................... 118
Europium (Time Resolved Fluorescence)............................ 118
Fluorescence Polarization (FP- infinite
Absorbance.......................................................................... 120
Glow Type Luminescence................................................... 121
ATP Glow Luminescence .................................................... 121
Flash Type Luminescence .................................................. 122
Dual Color Luminescence (e.g. BRET
"On the Fly" Measurements ............................................... 123
Cuvette Measurements (infinite
Injector..................................................................................125
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
®
................................................112
®
F200 only)........... 119
TM
) .......................... 123
®
M200 only)...................... 124
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