tecan infinite 200 Instructions For Use Manual page 131

Uniformity
Measurement Parameters:
Plate Layout:
<> 1 2
A
B
C
D
E
F
G
H
Material/Reagents:
Calculation of Uniformity:
2008-07
Perform the following measurement to determine the Uniformity:
Parameter
Reading Mode
Exc Wavelength
Em Wavelength
Number of flashes
Integration Time
Settle Time
Gain
Plate Type
Pipette 200 µl of 1 nm Fluorescein or the blank solution (0.01 M NaOH) into the
appropriate wells according to the plate layout:
3 4 5
1 nm Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377)
0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045)
1 Greiner 96-well plate black
200 µl Pipette + tips
stdev
= F
Uniformity (%)
mean
mean Average RFU value of wells filled with fluorophore
F
stdev Standard deviation of RFU values of wells filled with fluorophore
F
The result of the formula determines the uniformity in % CV.
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
Setting
Fluorescence Top
infinite
infinite
infinite
infinite
25
40
0
Optimal
GRE96fb
6 7 8
* 100
F
8. Quality Control
®
: 485 (20 nm)
F200
®
: 485 nm
M200
®
: 535 (25 nm)
F200
®
: 535 nm
M200
9 10 11 12
131