Defining Filter Slides (Infinite ® F200 Only); About Filters; Fluorescence Filters; Absorbance Filters - tecan infinite 200 Instructions For Use Manual

4. Defining Filter Slides
(infinite

4.1 About Filters

4.1.1

Fluorescence Filters

4.1.2

Absorbance Filters

4.2 Filter Slide and Filter Orientation

4.2.1

Filter Slide

Excitation part
2008-07
®
F200 only)
The optical filters (bandpass style) in a filter slide are specially designed for
fluorescence measurements. The spectral rejection and the bandwidth of the
fluorescence filters are optimized for achieving excellent sensitivity.
Contact TECAN for filters other than those supplied on the delivered filter slides.
Bandpass filters, which are commonly used in microplate readers for absorbance
measurements, usually have a bandwidth of 10 nm. Therefore it is not
recommended to use fluorescence filters for absorbance measurements because
the bandwidth (FWHM) is usually larger than 10 nm. This could cause a bright
value error or low OD values when measuring dyes with narrow peaks.
®
The infinite F200 filter slide consists of an excitation and an emission part. The
filter slide enables the user to work with four independent and non-
interchangeable excitation/emission filter pairs, which can be defined on positions
1 to 4. The information about the inserted filters is saved on the integrated
microchip.
®
F200 : Filter slide
infinite
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
4. Defining Filter Slides (infinite® F200 only)
Emission part
55