tecan infinite 200 Instructions For Use Manual page 28

2. General Description
Priming Procedure (hardware button):
STOP
Priming Example – Operational Sequence of Pump System
28
Priming can also be performed without using the software. Priming parameters
can be stored on the injector by clicking Save as Default on the Prime tab of the
Injector Maintenance dialog box of the i-Control software (in the Settings menu,
click Injectors... and the Injector Maintenance dialog box appears). Press the
Prime/Wash button on the injector box to start the priming sequence using the
default parameters, (see Figure 2-4: Injector-box with injector in 'service position',
page 24). The injector must be connected the instrument and the instrument must
be switched on. Start the prime procedure by pressing the Prime/Wash button for
less than 3 seconds.
Visually inspect the syringes for air bubbles. Any bubbles should be removed
after priming to ensure good injection performance.
After a successful priming procedure, reinsert the injector into the instrument.
Close the lid of the pump module completely before starting a measurement. The
injectors are now ready to use.
When starting a measurement with the actions 'injection' or 'dispense', 5 µl of
liquid are dispensed into a disposable container on the plate carrier before
starting 'injection' or 'dispense'. This initial dispense step makes sure that the
injection/dispense conditions are equal for each well.
Close the lid of the pump module (injector box) completely before
The following example describes the operational sequence of the pump system
when performing a prime step with 500 µl.
•
The system is already washed (syringe is empty; piston in upper position):
•
The first action is always that the syringe has to be filled completely with
liquid. The piston therefore moves down to the lowest position to fill
syringe completely (volume 1 ml).
•
Now the system prepares for the priming step. The piston makes space
for the selected prime volume: The selected prime volume is ejected:
500 µl – the piston moves up.
•
The piston moves down again to prime the syringe with the selected
volume. The syringe is now completely filled.
•
After finishing priming the last action is to eject 5 ml. This step makes
sure that the injection/dispense conditions are equal for each well (for
details please refer to 'Waste tub')
Be aware that for selected prime volume of 500 µl, 1500 µl liquid are needed
due to the initial filling step of the syringes.
•
The system is not washed (the syringe is partly filled with liquid, the piston is
not in the upper position):
•
The first action is always that the syringe is emptied. The piston therefore
moves up to the highest position to empty the syringe.
•
The next actions are similar to case 1.
For a selected prime volume of 500 µl, a minimum volume of 1500 µl of
liquid is required to perform a complete priming step.
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
Caution
starting a measurement.
Note
2008-07