tecan infinite 200 Instructions For Use Manual page 27

Priming
Prime
2008-07
Before the injection system can be used, an initial filling step (priming) is needed
to remove all air and to completely fill the system with liquid.
It is recommended to perform a washing step before priming.
Priming can be performed by using the i-Control software or by using the
hardware buttons on the injector box:
Priming procedure (general):
1. Fill the storage bottles with the necessary reagents and insert the feeding
tube(s). Make sure, that the tube(s) reaches the bottom of the bottle.
2. Remove the injector from the carrier slot and insert it into the service
position of the injector box.
3. Put an empty container under the injector.
Priming procedure (i-Control):
1. Adjust parameters at the prime tab of the injector maintenance dialog box
in the settings menu
2. Activate the priming procedure by clicking the 'Start prime' button in the
injector maintenance dialog box.
3. Visually inspect the syringes for air bubbles. Any bubbles should be
removed after priming to ensure good injection performance.
Select one of the injectors Injector A or Injector B or Injector A and B.
Select the 'Prime Volume' (5 -60000 µl)
Select the 'Prime Speed' (100 - 300 µl/sec).
Select the 'Refill Speed' (100 – 300 µl/sec.) or select 'Refill Speed equal to
Prime Speed'.
Start prime by clicking the 'Start prime' button.
Click the 'Save as default' button to save the selected settings to the
corresponding hardware button (A or B) on the injector box. When using the
hardware buttons for priming, these settings will be applied.
Select 'Close' to exit the dialog box
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
2. General Description
27