Light Source System - tecan infinite 200 Instructions For Use Manual

5.2.1

Light Source System

Flash lamp
Condenser
Band Pass Filter
Excitation Filter
Flash Monitor
2008-07
Flash light enters the optical system by being focused through an orifice
containing the filter. This opening acts as a color specific light source.
®
The light source system is built from the following components:
infinite F200
•
Flash lamp
•
Condensing Optics
•
Excitation Filters
•
Flash lamp Monitor
®
The utilizes a high energy Xenon arc discharge lamp (Flash lamp).
infinite F200
The flash sparks across a small gap between two electrodes. The lamp bulb
contains a high pressure Xenon atmosphere. The flash decays within some
microseconds.
The flash frequency is 40 Hz.
®
The uses the Flash lamp for fluorescence and for absorbance
infinite F200
measurements, although pulsed illumination is a must only for TRF. The main
benefits of this singular kind of lamp are:
1. High intensity from the deep UV to the near IR
2. Very long lifetime
3. Many applications - only one kind of lamp
4. No warm up time required
Condenser type optics focuses the light through the entrance orifice to the
fluorescence optical system.
In both fluorescence and absorbance applications, optical filters of band pass
type are necessary to select the useful wavelengths from the Flash lamp
spectrum. Filters are mounted in removable slides.
In many cases, fluorescence emission spectra do not depend on the exact
excitation wavelength. Therefore, for a maximum total fluorescence signal,
relatively broad excitation band pass filters (10 – 40 nm) may be used.
The spot diameter for the
2 mm for the bottom optics.
The light energy of single flashes may fluctuate slightly. To take these variations
into account, a silicon photodiode monitors the energy of every single flash.
Fluorescence measurement results are compensated correspondingly.
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
®
is about 2 mm for the top optics and
infinite F200
5. Optical System
73