tecan infinite 200 Instructions For Use Manual page 134

8. Quality Control
Material/Reagents:
Calculation of Detection Limit (Sensitivity):
Uniformity
Measurement Parameters:
Plate Layout:
<> 1 2
A
B
C
D
E
F
G
H
Material/Reagents:
Calculation of Uniformity:
134
25 nm Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377)
0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045)
1 Greiner 96-well plate µClear, black with transparent bottom
200 µl Pipette + tips
See 8.3.1 Fluorescence Top: Sensitivity.
Perform the following measurement to determine the Uniformity:
Parameter
Reading Mode
Exc Wavelength
Em Wavelength
Number of flashes
Integration Time
Settle Time
Gain
Plate Type
3 4 5
Filling volume: 200 µl
25 nm Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377)
0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045)
1 Greiner 96-well plate µClear, black with transparent bottom
200 µl Pipette + tips
See 8.3.1 Fluorescence Top: Uniformity
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
Setting
Fluorescence Bottom
®
infinite F200
®
infinite M200
®
infinite F200
®
infinite M200
25
40
0
Optimal
GRE96fb
6 7 8
: 485 (20 nm)
: 485 nm
: 535 (25 nm)
: 535 nm
9 10 11
2008-07
12