Figure 10-1. Uv Absorption - Teledyne T101 Operation Manual

Teledyne ML - T101 Operation Manual

Figure 10-1. UV Absorption

The equation for defining the average intensity of the UV light (Ia) is:
[
= −
Ia I 1
0
Where: (Equation 10-2)
=
I Intensity of the excitation UV light.
0
=
a the absorption coefficient of SO
=
SO Concentration of SO
2
=
x the distance between the UV source and the SO
affected (path length).
The second stage of this reaction occurs after the SO
Because the system will seek the lowest available stable energy state, the SO
quickly returns to its ground state (Equation 10-3) by giving off the excess energy in the
form of a photon (hν). The wavelength of this fluoresced light is also in the ultraviolet
band but at a longer (lower energy) wavelength centered at 330nm.
SO *
  → 
2
The amount of detectable UV (F) given off by the decay of the SO
at which this reaction occurs (k).
( (
−
exp ax SO
.
2
in the sample chamber.
2
SO hv
+
2
Principles Of Operation
]
) )
2
molecule(s) being
2
reaches its excited state (SO
2
330 nm
(Equation 10-3)
* is affected by the rate
2
*).
2
* molecule
2
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