Principles Of Operation
10.2.5. OPTICAL LENSES
Reference
Detector
If source UV is unfocused,
Reference Detector only sees a
small portion of emitted light
Figure 10-6. Effects of Focusing Source UV in Sample Chamber
252
5
10
10
4
3
10
10
2
10
1
1
0
100
200
Figure 10-5. PMT Optical Filter Bandwidth
Two optical lenses are used to focus and optimize the path of light through the sample
chamber.
If source UV is unfocused, PMT
receives fluorescence from area
outside Reference Detector's view
When source UV is focused,
Reference Detector sees
most of the emitted light
A lens located between PMT and the sample chamber collects as much of the fluoresced
UV created there as possible and focuses it on the most sensitive part of the PMT's
photo cathode.
Teledyne ML - T101 Operation Manual
PMT OPTICAL FILTER
BANDWIDTH
SO
* FLUORESCENT
2
SPECTRUM
300
400
WAVELENGTH (nm)
When source UV is focused, PMT
and Reference Detector view
similar volume of SO
PMT
500
*
2
UV Source
214 nm
Lens
Filter
330 nm
Filter
PMT Lens