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COULTER EPICS XL-MCL
Beckman Coulter COULTER EPICS XL-MCL Manuals
Manuals and User Guides for Beckman Coulter COULTER EPICS XL-MCL. We have
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Beckman Coulter COULTER EPICS XL-MCL manuals available for free PDF download: Service Manual, Getting Started
Beckman Coulter COULTER EPICS XL-MCL Service Manual (754 pages)
Flow Cytometer
Brand:
Beckman Coulter
| Category:
Laboratory Equipment
| Size: 14.56 MB
Table of Contents
Table of Contents
5
Warnings and Precautions
2
Revision Status
3
Table of Contents
5
Illustrations
30
Housing
31
Locations
31
Module
32
Open
32
View
33
View
38
Contents
41
1 Introduction
43
Manual Description
43
Intended Audience
43
Notification of Updates
43
Scope
43
Organization
44
Numbering Format
45
Special Headings
45
Attention
46
Caution
46
Conventions
46
Important
46
Note
46
Warning
46
Safety Precautions
47
Electronic
47
Special
47
Biological
48
Laser
48
Laser Beam
48
Radiation Hazards
49
Troubleshooting
53
Mesh
56
Star
56
Ring
56
Diagram
56
Removed
56
2 Instrument Description
57
System Overview
57
Components
57
Function
57
Sample Flow
57
Segmenting Valve/Positions Flow
57
Sheath Flow
57
Cytometer (All Signal Processing Electronics)
58
Power Supply Module
58
Computer Workstation
59
MCL Option - Theory of Operation
59
Cytometer
63
Flow Cell
64
Laser
64
Optics
64
Pinhole Assembly
65
Data Acquisition Card Cage
66
Forward Scatter (FS) Detector
66
Laser Intersection
66
Signal Processing
66
Amplifier (Amp)/Signal Conditioner Card
68
Trans Data Acquisition Card
70
System Interface Card
71
Cyto Transputer Card
72
MCL Interface Card
73
Card
74
Optical Transprocessor Extended Memory Cards
74
Opto Transprocessor EXMEM Card
74
Fiber Optic Interface Card
75
Sensor Card
75
Solenoid Power Distribution Card
75
Power Supply Module
77
Components
77
Description
77
Linear Power Supply
80
Workstation
81
Components
81
Standard Configuration
81
Options
82
Networking
83
Introduction
83
Purpose
83
Mesh
84
Star
84
Topologies
84
Linear Bus
85
Bridges, Repeaters, Routers
86
Ring
86
Dedicated Vs. Non-Dedicated Server
87
Lantastic™ Network Operating System
87
Specifications
87
Front
89
Assembly
93
3 Installation Procedures
95
Part A: Instrument Installation
95
Pre-Site Inspection
95
Space and Accessibility
95
Power Requirements
96
Environment
97
Optional Computer Peripherals
97
Unloading and Moving the Instrument
97
Inspection Report
98
Installation Assistance
98
Preinstallation Checks
99
Condition of Cartons Received
99
Education Center Training
99
Optional Computer Peripherals
99
Pre-Site Inspection Compliance
99
Supplies
99
Initial Installation
101
Cytometer
101
Power Supply Module
101
Tools/Supplies Needed
101
Unpacking
101
Power Supply Installation
102
Cytometer Installation
103
Remove Covers to Provide Needed Access
103
Remove the Optical Bench Shipping Tie-Down in the Front
108
Remove the Optical Bench Shipping Tie-Down in the Back (Behind the Laser)
109
Prepare Components Inside the Lower Pneumatics Drawer for Operation
112
Prepare the Data Acquisition Card Cage for Operation
112
Reinstall the MCL CPU Card (if Removed Earlier)
112
Prepare the Segmenting Valve for Operation
113
Prepare Components Inside the Upper Pneumatic Drawer for Operation
114
Fill the Reagent Containers
115
Fill the Sheath Container
115
Fill the Cleaning Agent Container
116
Workstation Installation
117
Initial Setup
117
Connecting the Monitor Cables
118
Keyboard Connection
118
Monitor Connections - Overview
118
Monitor Connections: 17-Inch Flat Panel Thin Film Transistor (TFT) Monitor
118
Mouse Connection
118
Connecting the Monitor Cables
119
Monitor Connections: 22-Inch CRT Monitor
119
Printer Option
119
Cable/Tubing Connections
121
Connect Cables
121
Laser Umbilical Cable
121
Analog Cable
123
Logic Cable
123
Make Appropriate MCL Connections
123
Power Module Control Cable
123
Fiber Optic Interface Cables
124
CYT12 Cable
125
Power Cords
125
Connect Tubings
125
Prepare the Waste Container for Tubing Connection
125
Tubing Connections
126
Software Installation
129
SYSTEM II Software Installation
129
Tools/Supplies Needed
129
Operational Checks
131
Preliminary Operational Checks
131
Purge Air Bubbles from the Sheath Filter
131
Tools/Supplies Needed
131
Functional Verification
133
Initial System Verification
133
Power Supply Verification
133
Trans Data Acquisition Card Offset Verification
133
Front Covers
134
Lower the Data Acquisition Card Cage
134
Reinstall Covers and Panels
134
Upper Pneumatic Drawer
134
Left-Side Cover
135
Power Supply Cover
135
Right-Side Cover
135
Top Cover
135
Clean up
136
Complete the System Verification Procedure (SVP)
136
Part B: Upgrades and Options Installation
137
Mcl Option Upgrade
137
Cytometer Upgrade
137
Preparation
137
Purpose
137
Tools/Supplies Needed
137
Power Supply Module Upgrade
138
MCL Alignment
139
Operational Verification
139
Power Supply Voltage Verification and Adjustment
139
Reinstall Covers
139
System Verification
140
4-Color Pmt Upgrade
141
Hardware Installation
141
Preparation
141
Purpose
141
Tools/Supplies Needed
141
Amp/Signal Conditioner Card Calibration
143
PMT Operational Alignment
143
Reinstall Covers
143
System Verification
143
Flowcentre ™ II MULTIMEDIA WORKSTATION UPGRADE
145
Mouse Connection
145
Opto Transprocessor EXMEM or EXMEM II Card Retrieval and Installation
145
Preparation
145
Purpose
145
Tools/Supplies Needed
145
Keyboard Connection
146
Monitor Connection (New or Existing)
146
Cable Connections
147
Fiber Optic Interface Cables
147
CYT12 Cable
148
Power Cord
148
SYSTEM II Software Installation
148
EXPO32™ Software Option Installation
149
System Verification
149
3.11 Bar-Code Printer Option
151
Printer Setup
151
Purpose
151
Tools/Supplies Needed
151
Media Calibration
153
Label Tear-Off Position Adjustment
154
Printer Testing
154
XL-MCL Flow Cytometer Connection
154
Printer Thermal Head Adjustment
155
Ribbon Replacement
155
Top-Of-Label Position Adjustment
155
3.12 Bar-Code Hand-Held Scanner Option
157
Installation
157
Purpose
157
Tools/Supplies Needed
157
Verification
158
3.13 Serial/Parallel Adapter Interface Card Option
159
Installation
159
Purpose
159
Removal
159
Tools/Supplies Needed
159
Verification
160
Network Setup Using Microsoft Windows 95 or
161
Purpose
161
Software Installation
161
Tools/Supplies Needed
161
Cable Connections
162
Set up Hard Drives and Printers
162
Define the Computer Name and Workgroup
163
Establish Control
163
Map a Network Shared Drive, if Desired
163
CD-ROM Usage in DOS
165
Hardware Installation
165
Lantastic ® CLIENT/SERVER SOFTWARE OPTION, VERSION
165
Preparation
165
Software Installation, Client Setup
165
Tools/Supplies Needed
165
Software and Hardware Validation
169
Software Installation, Server Setup
169
Lantastic ® CLIENT/SERVER SOFTWARE OPTION, VERSION
171
Purpose
171
Tools/Supplies Needed
171
Installation
172
Lantastic Client Version for MS-DOS Installation
172
Lantastic Client/Server Version for Windows™ 95
172
Lantastic ® CLIENT/SERVER SOFTWARE OPTION, VERSION
173
Sybase Client/Server SQL Software Option
173
Installation
175
Purpose
175
Tools/Supplies Needed
175
Config.sys and Startnet.bat File Editing
176
Lantastic File Server Software Connection
176
Preparation
179
Purpose
179
Software Installation, Client Setup
179
Sybase Client/Server SQL Software Option
179
Tools/Supplies Needed
179
Software Installation, Server Setup
180
Software and Hardware Validation
181
3.19 Expo32™ Software Options
183
For EXPO32 ADC Installation Option
183
For Printer Installation
183
Preparation
183
Purpose
183
Software Installation
183
Tools/Supplies Needed
183
Verification
183
Operation
185
Laser
188
Cover
193
4 Service and Repair Procedures
195
Guidelines for Servicing the Instrument
195
Electronic Precautions
195
General Guidelines
195
Instrument Access
195
Laser Safety
195
Safety Precautions
195
Procedures
196
Instrument Performance Verification
197
Stage 1: Power off the Cytometer
197
Tools and Supplies
197
Power up Using SYSTEM
198
Stage 1: Restoring Power to the System
198
Stage 2: Power off the Workstation
198
Stage 2: Restoring Power to the Workstation
198
Stage 3: System Power down
198
Restoring Power to the Cytometer Only
199
Power down / Power up Using EXPO32™ ADC Software
200
Power down Using EXPO32 ADC Software
200
Stage 1: Power off the Cytometer (System Using EXPO32 ADC Software)
200
Stage 2: Power off the Workstation (System Using EXPO32 ADC Software)
200
Power up Using EXPO32 ADC Software
201
Stage 1: Restoring Power to the System (System Using EXPO32 ADC Software)
201
Stage 2: Restoring Power to the Workstation (System Using EXPO32 ADC Software)
201
Stage 3: System Power down (System Using EXPO32 ADC Software)
201
Restoring Power to the Cytometer Only (System Using EXPO32 ADC Software)
202
Prefinal Service Software Installation and Operation
203
If Using SYSTEM
203
Preparation
203
Purpose
203
Tools/Supplies Needed
203
If Using EXPO32™ ADC Software
204
Installation
204
If SYSTEM II Is the Customer's Preferred Operating System
205
Operation
205
Wrap up
205
If EXPO32 ADC Is the Customer's Preferred Operating System
206
Cover Removal and Reinstallation
207
Locating a Procedure
207
Overview
207
Purpose
207
Covers
208
Access Inside the Cytometer
209
Power Supply Module Covers
210
Procedure 1
211
How to Remove and Reinstall the Cytometer Top
211
Removing the Cytometer Top
211
Tools/Supplies
211
Cytometer Interlock
212
Reinstalling the Cytometer Top
212
Procedure 2
213
How to Remove and Reinstall the Cytometer Right-Side
213
Removing the Cytometer Right-Side
213
Tools/Supplies
213
Reinstalling the Cytometer Right-Side
214
Procedure 3
215
How to Remove and Reinstall the Center
215
Tools/Supplies
216
Procedure 4
216
How to Properly Open and Close the Cytometer Reagent
216
Opening the Reagent
216
Closing the Reagent
218
Procedure 5
219
How to Remove and Reinstall the Cytometer Upper Rear
219
Removing the Cytometer Upper Rear
219
Reinstalling the Cytometer Upper Rear
220
Procedure 6
221
How to Remove and Reinstall the Cytometer Lower Rear
221
Removing the Cytometer Lower Rear
221
Reinstalling the Cytometer Lower Rear
222
Procedure 7
223
How to Remove and Reinstall the Manual Sample Station, Cytometer with MCL Option
223
Removing the Manual Sample Station on a Cytometer with the MCL Option Installed
223
Reinstalling the Manual Sample Station on a Cytometer with the MCL Option Installed
225
Procedure 8
226
How to Remove and Reinstall the Manual Sample Station, Cytometer Without MCL Option
226
Removing the Manual Sample Station on a Cytometer Without the MCL Option
226
Procedure 9
229
How to Remove and Reinstall the Lower Pneumatics Drawer
252
Removing the Lower Pneumatics Drawer on a Cytometer with the MCL Option Installed
252
Tools/Supplies Needed
252
Removing the Lower Pneumatics Drawer on a Cytometer Without the MCL Option
255
Reinstalling the Lower Pneumatics Drawer
256
How to Remove and Reinstall the Upper Pneumatics Drawer EMC Shield
258
Procedure 18
258
Removing the Upper Pneumatics Drawer EMC Shield
258
Tools/Supplies Needed
258
Reinstalling the Upper Pneumatics Drawer EMC Shield
259
How to Remove and Reinstall the Power Supply Module Three-Side Cover
260
Interlock Switch for the Power Supply Module
260
Procedure 19
260
Removing the Power Supply Module's Three-Sided Cover
260
Tools/Supplies Needed
260
Reinstalling the Power Supply Module's Three-Sided Cover
261
Close the Front Door
262
How to Open and Close the Power Supply Module Front Door
262
Open the Front Door
262
Procedure 20
262
Tools/Supplies Needed
262
Argon Laser Head or Power Supply Replacement
263
Laser Power Supply
263
Preparation
263
Purpose
263
Removal
263
Tools/Supplied Needed
263
Installation
264
Laser Head
264
Removal
264
Installation
265
Wrap up
265
Flow Cell and Beamshaper Replacement And/Or Alignment
267
Preparation
267
Purpose
267
Tools/Supplies Needed
267
Flow Cell Replacement
268
Beamshaper Assembly Removal, Replacement, and Alignment
269
Alignment
270
Install Alignment Tools
270
Reinstall Covers
272
System Verification
272
Laser Beam Steering
273
Optical Alignment
273
Preparation
273
Purpose
273
Tools/Supplies Needed
273
Forward Scatter (FS) Mask Alignment
274
How to Proceed
276
Preliminary Voltage Checks
277
Preparation
277
Purpose
277
Sample and Sheath Sensor Calibration
277
Tools/Supplies Needed
277
Calibration
278
Operational Verification
280
Reinstall Assemblies and Covers
281
System Verification
281
Carryover Test
283
Preparation
283
Purpose
283
Tools/Supplies Needed
283
Procedure
284
Using the Manual Sample Station
284
Using the MCL Sample Station
284
Peek Tubing Replacement
287
Preparation
287
Purpose
287
Removal
287
Tools/Supplies Needed
287
Installation
289
Reinstall Covers
291
System Verification
291
4.10 Sheath Pressure/Sample Station Vacuum Calibration
293
Calibration
293
Preparation
293
Purpose
293
Reinstall Covers
293
System Verification
293
Tools/Supplies Needed
293
Verification
293
4.11 Segmenting Valve Cleaning
295
Front Segmenting Pad
295
Preparation
295
Purpose
295
Removal
295
Tools/Supplies Needed
295
Middle Segmenting Pad
296
Rear Segmenting Pad
296
Clean the Shaft
297
Installation
297
Middle Segmenting Pad
297
Rear Segmenting Pad
297
Complete the Installation
298
Front Segmenting Pad
298
Reinstall Covers
298
Verification
298
4.12 Mcl Option Field Adjustment
299
MCL TERMINAL Program Setup
299
Preparation
299
Purpose
299
Tools/Supplies Needed
299
Carousel Tube-Position Sensor Adjustment
300
Finger Adjustment
301
How to Proceed
302
MCL Sample Probe Adjustment
302
4.13 Vortexer Foot Assembly Replacement
303
Preparation
303
Purpose
303
Removal
303
Tools/Supplies Needed
303
Installation
304
Reinstall Covers
305
Verification
305
4.14 Trans Data Acquisition Card Replacement And/Or Calibration
307
If Replacing the Trans Data Acquisition Card
307
Preparation
307
Purpose
307
Tools/Supplies Needed
307
Access the Prefinal Menu
308
Calibration
308
If Calibrating Without Replacing the Trans Data Acquisition Card
308
Load the Customer's Preferred Operating System
310
Reinstall Cover
310
System Verification
310
4.15 Amp/Signal Conditioner Card Replacement And/Or Calibration
311
If Replacing the Amp/Signal Conditioner Card
311
Purpose
311
Tools/Supplies Needed
311
Calibration
312
If Calibrating Without Replacing the Amp/Signal Conditioner Card
312
Verify Acceptable Histograms
314
Reinstall Cover
316
System Verification
316
4.16 Preparing Instrument for Shipment
317
Procedure
317
Purpose
317
Tools/Supplies Needed
317
4.17 Instrument Shutdown
319
Procedure
319
Purpose
319
Tools/Supplies Needed
319
4.18 Three-Wire Circuit Analyzer Test
321
Procedure
321
Purpose
321
Tools/Supplies Needed
321
Procedure
322
Reverse/Shorted Ground/Neutral Test
322
Tools/Supplies Needed
322
4.19 Power Supply Replacement
323
Preparation
323
Purpose
323
Tools/Supplies Needed
323
Removal
324
Replacement
324
System Verification
324
+24 VDC Power Supply
325
4.20 Power Supply Voltage Verification and Adjustment
325
Preparation
325
Purpose
325
Tools/Supplies Needed
325
Voltage Verification
326
Voltage Adjustment
327
Preparation
328
VDC Power Supply
328
Voltage Adjustment
328
Voltage Verification
328
+15 VDC Voltage Verification
329
Voltage Adjustment
329
MCL Power Supply
330
Preparation
330
Voltage Verification
330
Voltage Adjustment
331
4.21 RE-IMAGING Flowcentre™ COMPUTERS
333
Preparation
333
Purpose
333
Re-Imaging a Flowcentre Desktop Computer Without a Cache Controller Card
333
Re-Imaging a Flowcentre II Tower Computer
333
Tools/Supplies Needed
333
Re-Imaging a Flowcentre Desktop Computer with a Cache Controller Card
334
4.22 Universal CD-Rom Driver Setup
337
Driver Installation
337
Preparation
337
Purpose
337
Tools/Supplies Needed
337
4.23 Bar-Code Scanner Eeprom Custom Programming
339
Preparation
339
Purpose
339
Tools/Supplies Needed
339
Computer Setup
340
Custom Programming Sequence
341
If You Do Not Want to Store this Custom Configuration
342
If You Want to Store this Custom Configuration (Optional)
342
Reinstall Covers
343
Verification
343
Maintenance Procedures
347
Preparation
347
Purpose
347
Required Service Form
347
Static Tests
347
System Verification Procedure (Svp)
347
Tools/Supplies Needed
347
Acquisition, CV Analysis and Carryover Check
348
Power up Tests
348
Trans Data Acquisition Card Offset Verification
348
Using the Manual Sample Station
348
Using the MCL Sample Station
350
Interlock Checks
351
On-Site Close out
352
Workstation Computer Test
352
Preventative Maintenance Inspection (Pmi)
353
Procedure
353
Purpose
353
Tools/Supplies Needed
353
System Verification
354
Engineering Schematics
359
Schematics
359
Cleanse Low
367
Error Messages
367
Level Sense Indicators
367
Level Sense Indicators and Error Messages
367
Sheath Low and Waste Full
367
Troubleshooting
367
About the Error Messages Table
368
ADC Zero Adjust
379
Prefinal Service Software - a Troubleshooting Tool
379
Preparation
379
Purpose
379
Tools/Supplies Needed
379
Amp Gain Control
380
Amp Saturation Test
380
Circuit Card Checked in this Test
380
Circuit Cards Checked in this Test
380
Purpose
380
Response if Test Fails
380
Attenuator Control
381
Beeper Test
381
Circuit Card Checked in this Test
381
Circuit Cards Checked in this Test
381
Purpose
381
Response if Test Fails
381
Canyon Jumper Test
382
Circuit Card Checked in this Test
382
Circuit Cards Checked in this Test
382
Count Rate Test
382
DMA Acquisition - Not Used
382
Front Panel Test
382
Purpose
382
Response if Test Fails
382
Circuit Cards Checked in this Test
383
General Information
383
Response if Test Fails
383
Circuit Cards Checked in this Test
384
Grand Canyon Adjust
384
Grand Canyon Test
384
Purpose
384
Response if Test Fails
384
Circuit Cards Checked in this Test
385
Histogram Test
385
Initialize System
385
Purpose
385
Response if Test Fails
385
Components Checked in this Test
386
Laser Control
386
Options
386
Purpose
386
Response if Test Fails
386
Laser Warm up
387
Lock up Test - Not Used
387
MCL Bar Code Head Test
387
MCL Bar Code Test
387
Purpose
387
Error Index Numbers
388
MCL Burn in
388
MCL Carousel Label
388
Purpose
388
MCL Carousel Status
389
MCL Door Switch Test
389
MCL Finger Test
389
MCL Home Align
389
Purpose
389
Response if Test Fails
389
Summary of Test Sequence
389
Test Sequence
389
Controls
390
MCL Manual Control
390
MCL MIX Test
390
Purpose
390
Response if Test Fails
390
Sensor Status
390
MCL POWER up Status
391
MCL ROM Test
391
MCL Scan Reliability
391
MCL Stepper Noise Test
391
Purpose
391
Commands
392
MCL Terminal
392
Purpose
392
Circuit Cards Checked in this Test
395
Memory Test
395
Purpose
395
Circuit Cards Checked in this Test
396
Mike's Test -Not Used
396
New Board
396
Noise Test and Offset
396
Purpose
396
Response if Test Fails
396
Circuit Cards Checked in this Test
397
OPTO DMA Test
397
OPTO Interrupt Test
397
Purpose
397
Response if Test Fails
397
Circuit Cards Checked in this Test
398
OPTO Link Test
398
Purpose
398
Response if Test Fails
398
Response if Test Fails
399
Back
413
Module
647
Advertisement
Beckman Coulter COULTER EPICS XL-MCL Getting Started (112 pages)
Flow Cytometer, SYSTEM II Software
Brand:
Beckman Coulter
| Category:
Laboratory Equipment
| Size: 4.02 MB
Table of Contents
Warnings and Precautions
2
Revision Status
3
Table of Contents
5
Introduction
11
Description of Reporting
13
1 CONTROLS and Cytometer Ready and Laser on
15
Sample Flow Rate
17
Signal Amplitude
17
Operating Mode
18
Level Sense and Flow Indicators: XL-MCL Flow
19
Level Sense and Flow Indicators: XL Flow
19
Sample Stage Area
19
Computer
21
2 Learning the Basics
23
Learning the Basic Operating
23
Using the Sample
23
Test Tube and Sample
23
Putting a Tube on the Sample
24
Removing a Tube from the Sample
27
Using the Mcl
28
Putting Sample Tubes in a
28
Putting a Carousel in the Removing a Carousel from the
29
Bar-Code Labels
32
Putting a Bar-Code Label on a Sample
32
Using the Mouse
33
Selecting Screen
33
Symbols for Selecting Menu
33
Symbols for Selecting Screen
34
Moving between
35
Using Your Network Workstation and
35
3 Software Overview
37
Screen Features
37
Applications
37
Acquisition Application
38
Acquisition Setup Screen Menu
38
Acquisition Protocol Menu
39
Acquisition Screen Buttons
39
File Buttons
39
Histogram Display Buttons
39
Acquisition Buttons
40
Display and Region Buttons
40
Output Options Window
41
Cytometer Control Windows
41
Counters and Stops Window
41
Color Compensation Window
42
Discriminators Window
42
Signal Window
42
Listmode Application
43
Listmode Setup Menu
43
Listmode Screen Buttons
44
Histogram Display Buttons
44
Output and Region Buttons
44
Replay Options Buttons
44
Multigraph Application
45
Multigraph Screen Menu
45
Multigraph Screen Buttons
46
Histogram Display Buttons
46
Mode Buttons
46
Save and Print Buttons
46
Reports Application
47
Reports Screen Menu
47
Qc Application
48
QC Screen Menu
48
Utilities Application
49
Utilities Screen Menu
49
Pop-Up Windows
50
File Menu
52
3.10 Using the Keyboard
53
Utility Keys
53
Histogram Display Keys
53
Acquisition Keys
53
Selecting Menu Items with the Cursor Keys
54
Keyboard Shortcuts/Hot Keys
54
3.11 Responding to Prompts and Messages
55
Clearing the Prompt from the Screen
55
Editing the Default or the Information You Type
55
Entering Information
55
Prompts
55
Using the Default Information
55
Acknowledging a Message
56
Messages
56
3.12 Adjusting Cytometer Settings
56
Acquisition Cytosettings Screen
57
4 Creating Protocols
59
What Is Aprotocol
59
Creating and Editing Protocols
59
Selecting Signals
60
Naming Signals
60
Erasing Signals
61
Selecting and Assigning aux
62
Editing aux Parameter
63
Selecting and Assigning Ratio Parameter
63
Selecting and Assigning TIME Parameter
65
Activating PRISM in Acquisition
66
Activating PRISM in Listmode
66
Defining Histograms
67
Assigning Parameters and Titles to Histograms
67
Erasing Parameters and Histograms
69
Specifying Histogram Options
69
Histogram Resolution
70
Positives Analysis
70
Specifying Function Options
71
Saving or Updating Aprotocol
72
Saving a Protocol
72
5 Using Regions
73
Region Types
73
Acquisition Regions
73
Region List
73
One-Parameter Histogram Regions
74
Numeric and Linear Regions
74
Two-Parameter Histogram Regions
74
Numeric and Rectilinear Regions
74
Amorphous Region
75
Quad-Stat Region
75
Changing the Cursor Mode
76
Assigning Regions
76
Assigning Gates to Histograms
78
Assigning a Region as a Gate
79
Gating Equations
81
Sequential Gating
81
Cell-Seeker (Autogating) Regions
82
Assigning Autogating Regions
83
Cell-Tracker Regions
83
Assigning Cell-Tracker Regions
84
Prism Equation Regions
85
Assigning Prism Equations
86
Listmode Gating
86
Assigning Listmode Gates
87
Activating Listmode Gating
87
Assigning Negative Listmode Gates
87
Prime Regions
88
Assigning Prime Regions
88
Mapping Regions
88
Assigning Mapping Regions
89
5.10 Calibration Regions
90
5.11 Qc Regions
91
Assigning a QC Region
91
5.12 Lot Number Regions
92
6 Creating Panels
93
What Is Apanel
93
Panel Protocol Assignments
94
Primary Assignment
95
Control Assignment
95
Secondary Assignment
95
Next Assignment
95
Passing Cytometer Settings in a Panel
95
Passing Regions in a Panel
96
Cell-Seeker (Autogating) Regions in a Panel
96
Gating in a Panel
96
Passing Other Regions in a Panel
96
Passing Region Names in a Panel
96
Creating Apanel
97
Selecting Protocols
97
Making Assignments and Ordering Protocols
98
Saving a Panel
99
Adding Panels
100
Adding Protocols
101
7 The Operating System
103
The Xl or Xl-Mcl Flow Cytometer and the Ms-Dos ® Operating System
103
Restarting the Software from the MS-DOS Operating System
103
The Xl or Xl-Mcl Flow Cytometer and Microsoft Software
104
Windows Tm
104
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