Assigning Listmode Gates; Activating Listmode Gating; Assigning Negative Listmode Gates - Beckman Coulter COULTER EPICS XL Getting Started

Flow cytometer, system ii software
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Assigning Listmode Gates

1.
Run some of the sample.
2.
Draw a rectilinear or linear region
around the population you want to
include in the listmode file (all events
outside the region are excluded).
3.
Name the region. The name LISTGATE
(all caps) must be included in the
name.

Activating Listmode Gating

1.
Setup Screen
2.
Listmode Gate in the Functions
box to change it to ON.
OR
1.
[DISPLAY OPTION] until the
Discriminators box is displayed.
2.
[LISTMODE GATING] to change
it to ON.

Assigning Negative Listmode Gates

Negative listmode gating allows you to retain all the data outside of the gated region and to
exclude the data inside the region. Only linear or rectilinear regions can be assigned as a
negative listmode gate.
1.
Run some of the sample.
2.
Draw a linear or rectilinear region around the
population you want to exclude in the listmode
file (all events outside the region are included).
3.
Name the region. LISTGATENOT (all caps) or
LISTGATE- must be included in the region name.
PN 4237238BA
.
Protocols
tt
5
USING REGIONS
LISTMODE GATING
5-15

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