Example 2: Programmed Run - Gilson 155 User Manual

Operation
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Example 2: Programmed Run

This tutorial shows you how to program the
detector to:
• begin a run under one set of conditions
• complete the run by monitoring at different
wavelength and sensitivity parameters
Conditions
You are monitoring a three-peak sample in single
wavelength mode at:
• 254 nm
• a sensitivity of 0.01 AUFS
To accurately monitor the third peak, you want to
change the detection wavelength and sensitivity
values to:
• 320 nm
• a sensitivity of 0.02 AUFS
You'll switch to the new conditions at 2.5 minutes
into the program, because:
• you'll inject the sample at 1 minute into the run
• you know from previous runs that the third
peak elutes a little later than 1.5 minutes post-
injection
You'll also want to pause the run clock while the
sample is prepared and injected. To do this, you'll
set a wait event.
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