Baseline Noise - Gilson 155 User Manual

Troubleshooting
5-4
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Baseline Noise

• Allow each lamp to warm up for at least 1 hour.
• Eliminate drafty air currents and variations in
ambient temperature. Move the detector away
from windows or doors.
• Eliminate smoke from the environment.
• Check grounds on all instruments. Eliminate
ground loops by connecting the detector and
recorder to the same power outlet.
• Be sure solvents are degassed.
• Check fittings for a leak in system. Repair as
needed.
• If you see baseline deflection when back pressure
is exerted on the flow cell, you probably have
bubbles trapped in the cell. Try one of these
methods to eliminate bubbles:
- Increase back pressure on the cell by
connecting pressure regulator tubing to the
flow cell outlet.
- Increase back pressure by raising flow rate
and using a syringe or septum to momentarily
block exit stream.
- Flush flow cell using a syringe filled with
fresh solvent.
- Raise the fraction collector vial to a level
higher than the flow cell.
• A lamp may be weak. Refer to
Maintenance on page 4-22.
• Check flow cell for contamination. Refer to
Flow Cell Maintenance
5
Lamp
on page 4-2.