Diagnostics; Numeric Analyses On The 155 Detector - Gilson 155 User Manual

Uv/vis detectors
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Troubleshooting
5-8
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Diagnostics

The two beams of light that emerge from the beam
splitter form the reference and active beams. The
reference beam passes through an aperture to its
photodiode. The active beam passes through the
flow cell to its photodiode.
The flow cell receives your sample. The reference
photodiode monitors lamp intensity changes.
The photodiodes convert the light energy into an
electrical current. That current is proportional to
the intensity of the light.

Numeric Analyses on the 155 Detector

The Gilson service personnel can gain insight into
detector malfunction by examining voltage
percentages at various wavelengths. To provide
this information to Gilson, do the following:
1
Ensure that the flow cell is dry or contains only
HPLC-grade water.
2
Ensure that the detector is in single wavelength
mode. The detector uses the wavelength set in
single wavelength mode to generate the values
shown in the status mode display that you'll
access below.
To access single wavelength mode:
a) Press the Mode soft key at the first display of
the currently selected detection mode.
b) Press Single.
3
To select status mode, press Mode then Status.
5

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