Scan Wavelength Mode - Gilson 155 User Manual

Operation
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Scan Wavelength Mode

In scan wavelength mode, you can locate
wavelengths of maximum absorbance by scanning
peak material across a range of wavelengths.
Monitor your sample at any wavelengths between
190 and 700 nm, at one or two sensitivities. Scan
data are displayed on output channel 3.
Typically, scans are performed during peak elution,
although you can also scan discrete samples. See
Discrete Sample Scan
If the display on the next page is not shown, press
the Mode soft key at the first display of the current
detection mode. Then press Scan.
You should follow these guidelines when using the
scan mode:
• Optimal scans are achieved on broad Gaussian
peaks over a stable baseline
• It may take some trial and error to determine
when to begin the scan and what wavelengths
to scan to obtain the most informative data
• It is usually best to begin a peak scan just before
reaching the maximum peak height
• You should dedicate the scan run to that
purpose exclusively. You should not attempt to
quantify scanned peaks since detection is
interrupted while the scan is in progress
• You may achieve better results if you scan only
one peak per run. If you try to initiate a second
scan during the output of another scan, the
output will be interrupted and you will lose the
first set of scan data
To scan more than one peak, be aware of peak
retention times, scan duration times and scan
output times. Be sure that the elution of the
second peak does not interfere with either the
scan or the output of the first peak.
on page 3-38.
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