Gilson 155 User Manual page 136

Uv/vis detectors
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Troubleshooting
4
Press Next until you see a display like the
following:
5
Type 190 for the wavelength and press ENTER.
6
Wait until the AU reading stabilizes.
7
In tabular form, record the displayed AU, Active,
and Reference values. For the Active % and
Reference %, values up to 110% are within
normal operating parameters for the detector.
Values over 110% indicate there is too much
signal reaching the active and reference
photodiodes.
For the active and reference photodiode, the
software calculates the Active % and Reference
% as follows:
% = (Current Signal/Maximum Signal) * 100
8
Using 20 nm increments, repeat steps 5 through
7 to obtain AU, Active, and Reference values
for 190 to 700 nm.
9
When finished, press ESC to return to the
display for operation mode. The wavelength
shown on the display is the last one you entered
in status mode.
10 Enter an appropriate value for the wavelength
to use for operation mode.
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5-9

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