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Operation
Example Run
You can use your HPLC system to separate a
mixture of herbicides. You'll monitor the column
effluent at 235 nm. And, since the components are
present in low amounts, you'll monitor at high
sensitivity (0.005 AUFS). When monitoring at a
single sensitivity, you only need to connect output
channel 1 to your recorder.
Start detector
If necessary, start the detector as described on page
3-12.
Choose Single Wavelength Mode
If the following display is not shown, press ESC
until you see the following screen:
Set parameters
Follow these steps to set the detection parameters:
1
To set the monitor wavelength (235 nm), press
235 on the keypad, and then press ENTER.
2
To set the first sensitivity, press 0.005, and then
press ENTER.
3
Since you won't be charting the second
sensitivity, press ENTER to move the cursor to
the Peak width parameter.
4
At half height, the narrowest peak in the run is
10 seconds in duration. If necessary, press 10,
and then press ENTER.
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3-23
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