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Maintenance
4-4
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Acid cleaning
If you suspect that residual proteins contaminate
the flow cell, draw 50% nitric acid through the cell.
Acid cleaning is a more thorough technique,
though much more care must be taken.
CAUTION! Take all necessary precautions so that
acid does not come into contact with eyes, skin,
clothing and equipment surfaces.
To clean the inside of the flow cell:
1
Disconnect the inlet and outlet tubing from
your system.
2
Using a 1/4-28 coupler, connect a Luer-lock
fitting to the Omnifit-type fitting on the outlet
tubing. Then attach a large glass syringe to the
Luer-lock fitting.
3
Place the end of the inlet tubing into a reservoir
of 50% nitric acid.
4
Draw nitric acid through the cell.
CAUTION! Use nitric acid. Do not use
halogenated acid. Halogenated acid will damage
stainless steel.
WARNING! Do not pump acid through the cell.
Never put acid under pressure.
5
After the acid flush, draw HPLC-grade water
through the cell.
6
To be sure that all acid has been removed,
monitor the pH of the final outflow.
7
Reconnect the inlet and outlet tubing to the
system.
8
Check the detector's performance by running a
sample through the system.
4
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