Baseline Drift - Gilson 155 User Manual

Troubleshooting

Baseline Drift

• Allow each lamp to warm up for at least 1 hour.
• Eliminate changes in ambient temperature. If
drift is dependent on temperature, eliminate
drafts and avoid areas of direct sunlight.
• Check for contaminated or "bleeding" column.
If drift disappears after removing column
connections, regenerate or replace column.
• Be sure solvents are degassed.
• Check fittings for leak in system. Repair as
needed.
• If you see baseline deflection when back pressure
is exerted on the flow cell, you probably have
bubbles trapped in the cell. Try one of these
methods to eliminate bubbles:
- Increase back pressure on the cell by
connecting pressure regulator tubing to the
flow cell outlet.
- Increase back pressure by raising flow rate
and using a syringe or septum to momentarily
block exit stream.
- Flush flow cell using a syringe filled with
fresh solvent.
- Raise the fraction collector to a level higher
than the flow cell.
• Check pump or delivery system to determine
that flow rate is constant.
• A lamp may be weak. Refer to
Maintenance on page 4-22.
• Check flow cell for leak or contamination. Refer
to Flow Cell Maintenance
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Lamp
on page 4-2.
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