Discrete Sample Scan - Gilson 155 User Manual

Operation
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Discrete Sample Scan

To scan a single sample dissolved in a buffer, follow
these instructions.
1 Locate one 1/4-28 coupler and one male Luer
end-fitting from the accessory package. Make
the following connections:
• Attach the coupler to the detector outlet
• Connect the Luer end-fitting to the coupler
• Attach a syringe to the Luer end-fitting
2 Disconnect the detector inlet tubing from the
column. Immerse the end of the inlet tubing
into a pure buffer solution.
3 Draw the buffer solution through the inlet
tubing into the syringe.
4 From the first display for scan mode, press the
Zero soft key to perform a zero scan and to
reset the baseline of the trace at the monitor
wavelength to zero. The mobile phase spectrum
is automatically subtracted from the compound
spectrum during the scan.
5 Immerse the inlet tubing into the sample of
interest (in the same buffer).
6 Draw the sample solution through the inlet
tubing into the syringe. Be sure that the cell is
overfilled on both ends to eliminate bubbles
from the flow cell.
7 Press Scan to begin the sample scan.
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