Hitachi ZA3000 Series Instruction Manual page 348

Fig. 5-17 Working-curve Table Window
(8) In the [STD addition] field, select [Outer] and make entry for
[Blank Cup No.].
Use of the default value (No. 59) does not pose any
problem.
For [STD Cup No.], enter the cup No. of the unknown
sample to which each standard sample was added.
(9) Verify the method, place the standard-added unknown
samples, namely second and subsequent unknown
samples and blank sample in the sample tray, and then
start measurement.
No sample needs to be set in the cup No. specified for the
first unknown sample as the entire amount of the mixed
samples prepared by using flasks have already been set in
the autosampler.
NOTE: For the first unknown sample, select the one that
contains a matrix constituent having the average
composition of all unknown samples and whose
absorbance is not extremely high.
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(1)
5.1.5