Derivation Of Wbc-Related Parameters - Mindray BC-2800 Operator's Manual

HGB(g/L) = Constant×Log

3.4.3 Derivation of WBC-Related Parameters

WBC
9
WBC (10 / L) is the number of leukocytes measured directly by counting the white blood cells
passing through the aperture.
Note that NRBCs do not react with the lyse and can be mistaken by the analyzer for white
blood cells. If you observe NRBCs in the microscope, correct the system-generated result by
the following formula,
where WBC represents the system-generated white cell number, NRBC the number of
NRBCs counted in 100 white cells and WBC' the corrected white cell number.
WBC differentia
With the help of the diluent and lyse, this analyzer can size the white cells into three
sub-populations - lymphocytes, mid-sized cells (including monocytes, basophils and
eosinophils) and granulocytes. Based on the WBC histogram, this analyzer calculates Lymph
％, Mid％ and Gran％ as follows and express the results in percents.
Lymph%
Mid%
Gran%
where PL = particles in the lymphocyte region(
PM = particles in the mid size region(
PG = particles in the granulocyte region(
Having achieved the three parameters above, this analyzer proceeds to calculate the
Lymph# , Mid# and Gran# per the following equations and express them in
Understanding the System Principles
(Blank Photocurrent/Sample Photocurrent)
10
= ×
9
WBC n 10 /
100
×
＝
WBC ' WBC
＋
100
PL
=
+ +
PL PM PG
PM
= ×
+ +
PL PM PG
PG
=
+ +
PL PM PG
9
)
10 L /
9
10 L /
3-6
L
NRBC
×
100
100
×
100
9
)
10 L /
).
9
.
10 L /