To fix a rotation motor error on the Mindray BC-3000 Plus, follow these steps:

1. Check for a jammed sample probe: Open the front panel and inspect if the sample probe is jammed.
2. Verify signal line contact: Ensure there is no poor contact in the signal line.
3. Test the motor: Enter the “Service → System Test” screen and check the motor as instructed in Chapter 10.6. If the test result is normal, the error should be removed.
4. Inspect connections: Check for poor connections between the drive board and the CUP board.
5. Check for motor damage: If the above steps do not resolve the issue, examine whether the motor is damaged.
6. Seek assistance: If the problem persists, contact Mindray customer service or your local distributor for further support.

This answer is automatically generated

-1

Jean de Dieu HABIMANA

February 2, 2025

How Caused the not display the result for mindray Bc 2800 if there'snt error display messenges

1 comments:

Mr. Anderson

February 10, 2025

Possible causes for the Mindray BC-3000 Plus not displaying results without showing error messages include:

1. Abnormal QC Results – If quality control (QC) results are abnormal, the analyzer may not provide valid results. Steps such as re-running the control, checking calibration, and performing a background check may be needed.
2. Improper L-J Settings – Incorrect Levey-Jennings (L-J) settings could prevent proper data processing.
3. Communication Issues – If the analyzer is set to transmit data to an external computer via RS-232 but the connection is faulty, results may not appear on the screen.
4. Calibration Issues – If the analyzer requires calibration, it may not generate valid results.

Checking these factors and troubleshooting accordingly may help resolve the issue.

This answer is automatically generated

Albert

January 27, 2025

Comment supprimer les données sur Mindray BC 3000 plus

Harriet kyei Baffour

March 7, 2025

Rotation motor error

-1

1 comments:

Mr. Anderson

March 7, 2025

A rotation motor error for the Mindray BC-3000 Plus indicates issues such as a jammed sample probe, poor contact of the signal line, a damaged motor, or a poor connection between the drive board and the CUP board. To resolve this, check if the sample probe is jammed, perform a system test in the service menu, and ensure all connections are secure. If the issue persists, contact Mindray customer service or a local distributor.

This answer is automatically generated

Summary of Contents for Mindray BC-3000 Plus

Page 1 BC-3000 Plus Auto Hematology Analyzer Operation Manual...
Page 3 Mindray, nor the rights of others. Mindray does not assume any liability arising out of any infringements of patents or other rights of third parties.
Page 4 the product is used in accordance with the instructions for use. This equipment must be operated by skilled/trained medical professionals It is important for the hospital or organization that employs this equipment to carry out a reasonable service/maintenance plan. Neglect of this may result in machine breakdown or injury of human health.
Page 5 Return Policy Return Procedure In the event that it becomes necessary to return this product or part of this product to Mindray, the following procedure should be followed: Obtain return authorization: Contact the Mindray Service Department and obtain a Customer Service Authorization (Mindray) number.
Page 6 Company Contact Manufacture: Shenzhen Mindray Bio-Medical Electronics Co., Ltd. Mindray Building, Keji 12th Road South, Hi-tech Industrial Park, Address: Nanshan, Shenzhen, P.R.China,518057 +86 755 26582479 26582888 Phone: +86 755 26582500 26582501 Fax: EC-Representative: Shanghai International Holding Corp. GmbH(Europe) Eiffestraβe 80, 20537 Hamburg Germany...
Page 7: Table Of Contents
Table of Contents Using This Manual ................... 1-1 Introduction .................... 1-1 Who Should Read This Manual ............. 1-2 How to Find Information................. 1-3 Conventions Used in This Manual ............1-4 Special Terms Used in This Manual............1-5 Symbols ....................1-6 Understanding Your Analyzer..............
Page 8 Table of Contents Auto Clean Time .................. 5-26 Reagent Exp. Date................5-27 5.10 Report Title （external keyboard required）........5-29 5.11 Parameter Units ................... 5-31 5.12 Other ....................5-34 Operating Your Analyzer ................. 6-1 Introduction .................... 6-1 Initial Checks..................6-2 Power-on....................6-3 Daily Quality Control ................
Page 9 Table of Contents 10.11 Replacing the Filter of the Vacuum Chamber ........10-38 10.12 Using the [Flush] key ................. 10-39 10.13 Using the [Startup] key............... 10-40 Troubleshooting Your Analyzer ............11-1 11.1 Introduction ..................11-1 11.2 Errors without available error messages ..........11-2 11.3 Errors indicated by error messages .............
Page 11: Using This Manual
Using This Manual 1.1 Introduction This chapter explains how to use your BC-3000 Plus operation manual, which is shipped with your BC-3000 Plus hematology analyzer and contains reference information about the BC-3000 Plus and procedures for operating, troubleshooting and maintaining the analyzer.
Page 12: Who Should Read This Manual
Using This Manual 1.2 Who Should Read This Manual This manual contains information written for clinical laboratory professionals to: learn about the BC-3000 Plus hardware and software; customize system settings; perform daily operating tasks; perform system maintenance and troubleshooting.
Page 13: How To Find Information
Analyzer learn about how to define/adjust system settings Chapter 5 Customizing the Analyzer Software learn about how to use the BC-3000 Plus to perform your daily Chapter 6 Operating Your operating tasks Analyzer learn about how to review the saved analysis results...
Page 14: Conventions Used In This Manual
Using This Manual 1.4 Conventions Used in This Manual This manual uses certain typographical conventions to clarify meaning in the text: All capital letters enclosed in [ ] indicate a key name (either on the built-in keypad or the external keyboard), such as [ENTER]. All capital, bold and italic letters indicate a special operation defined in the following section, such as SELECT.
Page 15: Special Terms Used In This Manual
Using This Manual 1.5 Special Terms Used in This Manual When you read … It means … to press the arrow keys ([←][→] [↑][↓]) as needed to move the CLICK cursor to a certain software button on screen and press [ENTER].
Page 16: Symbols
Using This Manual 1.6 Symbols You will find the following symbols in this manual. When you see… Then… read the statement below the symbol. The statement is alerting you to an operating hazard that can cause personnel injury. read the statement below the symbol. The statement is alerting you to a possibility of analyzer damage or unreliable analysis results.
Page 17 Using This Manual BATCH CODE USE BY SERIAL NUMBER DATE OF MANUFACTURE TEMPERATURE LIMITATION CONSULT INSTRUCTIONS FOR USE THE DEVICE IS FULLY CONFORMANCE WITH COUNCIL DIRECTIVE CONCERNING IN VITRO DIAGNOSTIC MEDICAL DEVICES 98/79/EC. MANUFACTURER AUTHORISED REPRESENTATIVE IN THE EUROPEAN COMMUNITY IRRITATING SUBSTANCE THE FOLLOWING DEFINITION OF THE WEEE LABEL APPLIES TO EU MEMBER...
Page 18 Using This Manual TO THE ENVIRONMENT AND HUMAN HEALTH. MORE DETAILED INFORMATION WITH REGARD RETURNING RECYCLING THIS PRODUCT, PLEASE CONSULT DISTRIBUTOR FROM WHOM PURCHASED THE PRODUCT.
Page 19 Using This Manual Figure1-1 Back of the Analyzer （1） Equipotentiality. （2） Connect only to a properly earth grounded outlet; To avoid electric shock, disconnect power cord prior to removing or replacing fuse; Replace fuse only with the type and rating specified. （3）...
Page 20 Using This Manual consequences to the environment and human health. For more detailed information with regard to returning and recycling this product, please consult the distributor from whom you purchased the product. 1-10...
Page 21 Using This Manual Figure1-2 Inside right of the analyzer （5） To avoid being injured, do not put hand under the motor when the machine is running. 1-11...
Page 23: Understanding Your Analyzer
Understanding Your Analyzer 2.1 Introduction The BC-3000 Plus auto hematology analyzer is a quantitative, automated hematology analyzer and leukocyte differential counter for In Vitro Diagnostic Use in clinical laboratories.
Page 24: Intended Use
Understanding Your Analyzer 2.2 Intended Use The purpose of this analyzer is to identify the normal patient, with all normal system-generated parameters, and to flag or identify patient results that require additional studies. The analyzer is used for the quantitative determination of the following 19 parameters and 3 histograms of blood samples.
Page 25: User Interfaces
Understanding Your Analyzer 2.3 User Interfaces Figure2-1 Front view 1 ---- LCD 2 ---- Keypad 3 ---- Recorder 4 ---- Power indicator 5 ---- Aspirate key 6 ---- Sample probe...
Page 26 Understanding Your Analyzer Figure2-2 Back view 1 --- RS-232 Port1 2 --- Parallel Port 3 --- RS-232 Port2 4 --- Keyboard interface 5 --- Power Interface of Floppy Disk Drive 6 --- Safety labeling 7 --- Diluent inlet 8 --- Diluent sensor connector 9 --- Rinse sensor connector 10 --- Waste outlet 11 --- Rinse inlet...
Page 27 Understanding Your Analyzer Figure2-3 Inside front of the analyzer 1 --- Elevator motor 2 --- Sample probe 3 --- Probe wipe 4 --- WBC shielding box 5 --- RBC shielding box 6 --- Aspirate key...
Page 28 Understanding Your Analyzer Figure2-4 Inside right of the analyzer 1 --- Valve8 2 --- Volumetric metering unit 3 --- Vacuum chamber 4 --- Valve13 5 --- Valve14 6 --- Valve12 7 --- Valve11 8 --- Valve10 9 --- Valve2 10 --- Valve9 11 --- 50ul and 2.5ml motor 12 --- 10ml motor 13 --- 2.5ml syringe...
Page 29 Understanding Your Analyzer Figure2-5 Inside left of the analyzer 1 --- Fluid pump 2 --- Gas pump 3 --- Pressure chamber...
Page 30 Understanding Your Analyzer 2.3.1 LCD The LCD is located on the front panel of the analyzer. It displays all alphanumeric and graphic data. 2.3.2 Input Devices The input devices include the aspirate key, built-in keypad and PS/2 keyboard. Aspirate key The aspirate key is located behind the sample probe, as Figure2-6 shows.
Page 31 Understanding Your Analyzer Built-in keypad The 23-key keypad is located below the LCD, as Figure2-7 shows. Figure2-7 Built-in keypad PS/2 keyboard The analyzer can also be controlled by an external PS/2 keyboard that should be connected to the analyzer’s keyboard interface. See the table below for the correspondence between the keypad keys and the keyboard keys and for their functions.
Page 32 2.3.7 Power Supply for the Floppy Disk Drive It supplies power to the connected floppy disk drive. Only the drive power cable supplied by Mindray can be used. 2.3.8 Power Indicator The power indicator tells you whether the analyzer is on, off or in the screen saver mode.
Page 33: Instrument Software
Understanding Your Analyzer 2.4 Instrument Software 2.4.1 Main Screen After finishing the startup procedure, the analyzer enters the “Count” screen, which is the screen to be used most frequently, hence the name “Main screen”. The main screen is shown in Figure2-8. Error message area Status area Title area...
Page 34 Understanding Your Analyzer Count Mode Area The Count Mode area displays in which analysis (count) mode, whole blood or prediluted, the next sample is to be analyzed. Transmission status A live animation is displayed in this area when the transmission is in process. System Time Area The System Time area displays the system time (in the 24-hour format).
Page 35 Understanding Your Analyzer Count Sample Mode Sample Review Sample Table Review Review Search Review Search Table Review Sample Histogram Review Search Histogram Review L-J Analysis L-J/X/X-R Edit File 1 Quality Control L-J/X/X-R Count File 1 File 2 X Analysis L-J/X/X-R Graph File 1 File 2 File 3...
Page 36 Understanding Your Analyzer You can select the desired program as instructed below. If you want to… Select… analyze samples Count select an appropriate analysis mode Sample Mode review sample results Review run the QC program Quality Control customize system software Setup maintain/service the analyzer Service...
Page 37: Reagents, Controls And Calibrators
You should only use the Mindray-specified reagents (see Appendix B Specifications), which are formulated specifically for the fluidic system of your analyzer in order to provide optimal system performance.
Page 38 Read and follow the instructions for use to use the controls and calibrators. All references related to controls and calibrators in this manual refer to the controls and calibrators reagents specifically formulated for this analyzer. You should buy those controls and calibrators from Mindrayor Mindray-authorized distributors. 2-16...
Page 39: Understanding The System Principles
Understanding the System Principles 3.1 Introduction The two independent measurement methods used in this analyzer are: the Coulter method for determining the WBC, RBC, and PLT data; the colorimetric method for determining the HGB. During each analysis cycle, the sample is aspirated, diluted and mixed before the determination for each parameter is performed.
Page 40: Aspiration
Understanding the System Principles 3.2 Aspiration This analyzer can process two types of blood samples – whole blood samples and prediluted blood samples. If you are to analyze a whole blood sample, you can simply present the sample to the sample probe and press the aspirate key to aspirate 13µL of the sample into the analyzer.
Page 41: Dilution
Understanding the System Principles 3.3 Dilution Usually in blood samples, the cells are too close to each other to be identified or counted. For this reason, the diluent is used to separate the cells so that they are drawn through the aperture one at a time as well as to create a conductive environment for cell counting.
Page 42 Understanding the System Principles When analyzing a prediluted sample, you should first collect 20μL of capillary sample and dispense 0.7mL of diluent from this analyzer to predilute it. Then the analyzer aspirates 0.3ml of the pre-diluted sample for further dilution, as Figure3-2 shows. 20uL of capillary blood sample 0.7mL of diluent 1:36 dilution...
Page 43: Wbc/Hgb Measurement
Understanding the System Principles 3.4 WBC/HGB Measurement 3.4.1 Volumetric Metering An accurate cell count cannot be obtained unless the precise volume of diluted sample that passes through the aperture during the count portion of the analysis cycle (the count cycle) is known.
Page 44 Understanding the System Principles 3.4.2 Measurement Principles WBC measurement WBCs are counted and sized by the Coulter method. This method is based on the measurement of changes in electrical resistance produced by a particle, which in this case is a blood cell, suspended in a conductive diluent as it passes through an aperture of known dimensions.
Page 45 Understanding the System Principles (readings taken when there is only diluent in the bath). The HGB is calculated per the following equation and expressed in g/L. HGB(g/L) = Constant×Log (Blank Photocurrent/Sample Photocurrent) 3.4.3 Derivation of WBC-Related Parameters WBC (10 / L) is the number of leukocytes measured directly by counting the white blood cells passing through the aperture.
Page 46 Understanding the System Principles × Lymph Lymph × × Gran Gran WBC histogram Besides the parameters mentioned above, this analyzer also presents a WBC histogram, whose x-coordinate represents the cell volume（fL）and y-coordinate represents the number of the cells. The histogram is presented in the Analysis area of the “Count” screen when the analysis is done.
Page 47: Rbc/Plt Measurement
Understanding the System Principles 3.5 RBC/PLT Measurement 3.5.1 Volumetric Metering An accurate cell count cannot be obtained unless the precise volume of diluted sample that passes through the aperture during the count cycle is known. This analyzer uses a volumetric metering unit to control the count cycle and to ensure that a precise volume of sample is analyzed for the measurement.
Page 48 Understanding the System Principles 3.5.2 Measurement Principles RBC/PLT measurement RBCs/PLTs are counted and sized by the Coulter method. This method is based on the measurement of changes in electrical resistance produced by a particle, which in this case is a blood cell, suspended in a conductive diluent as it passes through an aperture of known dimensions.
Page 49 Understanding the System Principles Based on the RBC histogram, this analyzer calculates the mean cell volume (MCV) and expresses the result in fL . This analyzer calculates the HCT (%), MCH (pg) and MCHC (g/L) as follows: This analyzer calculates the HCT (%), MCH (pg) and MCHC(g/L) as follows: ×...
Page 50 Understanding the System Principles 3.5.4 Derivation of PLT-Related Parameters PLT (10 /L) is measured directly by counting the platelets passing through the aperture.。 Based on the PLT histogram, this analyzer calculates the mean platelet volume (MPV, fL). Platelet distribution width (PDW) is the geometric standard deviation (GSD) of the platelet size distribution.
Page 51: Wash
Understanding the System Principles 3.6 Wash After each analysis cycle, each element of the analyzer is washed. The sample probe is washed internally and externally with diluent; The WBC bath is washed with diluent and rinse; The RBC/PLT bath is washed with diluent and rinse; The metering tube is washed with rinse.
Page 53: Installing Your Analyzer
Installing Your Analyzer 4.1 Introduction This chapter introduces the installation procedure of the BC-3000 Plus. To ensure all system components functioning correctly verify system performance, Mindray-authorized representative will handle the installation and initial software setup. Installation by personnel not authorized or trained by Mindray may damage your analyzer.
Page 54: Installation Requirements
Installing Your Analyzer 4.2 Installation Requirements Before installation, you should ensure that the following space, power and environmental requirements are met. 4.2.1 Space Requirements Check the site for proper space allocation. In addition to the space required for the analyzer itself, arrange for at least 28 cm on each side, which is the preferred access to perform service procedures;...
Page 55 Installing Your Analyzer The specified temperature range is necessary to obtain reliable analysis results. Relative humidity: 30% -85%; Atmospheric pressure: 70 kPa -106 kPa; The environment should be as free as possible from dust, mechanical vibrations, loud noises, and electrical interference; Do not place the analyzer near brush-type motors, flickering fluorescent lights, and electrical contacts that regularly open and close;...
Page 56: Unpacking
Notify the Mindray customer service department or your local distributor immediately if you find anything missing; 3. Open the main box and check the items inside against the packing list. Notify the Mindray customer service department or your local distributor immediately if you find anything missing;...
Page 57 Installing Your Analyzer Never move the analyzer without draining the fluidic lines. For a short - distance moving on a smooth ground, you may use a trolley to facilitate the transportation; During the moving process, be sure to protect the LCD and the sample probe from excessive force and from contact with other objects;...
Page 58: Installation Procedure
Installing Your Analyzer 4.4 Installation Procedure 4.4.1 Releasing sample probe The sample probe tip is sharp and may contain biohazardous materials. Exercise caution to avoid contact with the probe when working around it. Before the analyzer is shipped out, the sample probe is fixed by a plastic cable tie to avoid damage during shipment.
Page 59 Installing Your Analyzer 2. Lift up the front panel latch as indicated in Figure 4-2 and open the front panel; Figure 4-2 3. Cut the plastic cable tie to release the probe, as Figure 4-3 shows; Figure 4-3...
Page 60 Installing Your Analyzer 4. The released sample probe is shown in Figure 4-4; Figure 4-4 Lift the front panel latch and close the front panel and then release the latch to lock it. Finally close the right door. 4.4.2 Connecting Reagent Containers Consider all materials (samples, reagents, controls, calibrators, or components that contain or have contacted human blood) as being potentially infectious.
Page 61 Installing Your Analyzer Be sure to use the manufacturer-specified reagents. Be sure to keep the reagents still for a while before using them. Be sure not to use expired reagents. To prevent contamination, be sure to tighten the container caps when the installation is finished.
Page 62 Installing Your Analyzer 3. Take out the lyse pickup tube with an orange connector (see Figure4-7) from the accessory box. Take out the lyse container, in which there should be enough lyse. Remove the container cap and insert the double-pronged end of the tube into the container and turn (clockwise) the tube’s cap onto the lyse container until properly secured;...
Page 63 Installing Your Analyzer Connecting diluent container 1. Take out the diluent pickup tube with a green connector (Figure 4-9) from the accessory box; 2. Take out the diluent container, in which there should be enough diluent, and place it on or below the countertop;...
Page 64 Installing Your Analyzer Figure 4-10 4. Locate the green fitting, marked RINSE, in the lower right corner of the back of the analyzer. Plug the blue connector of the tube into the fitting and turn it clockwise until properly secured; 5.
Page 65 Installing Your Analyzer 4.4.3 Installing Recorder Paper Follow the procedure below to install the recorder paper. Improper installation of recorder paper may jam the paper and/or result in blank printouts. 1. Locate the projecting part in the upper right corner of the recorder and press it in the direction shown in Figure 4-11 to open it;...
Page 66 Installing Your Analyzer Figure 4-12 The recorder paper is treated on one side for printing. To determine which side is the printing side, gently scratch both sides with nails and the one with visible nail trace left is the printing side. 3.
Page 67 Installing Your Analyzer 4. Close the recorder door, as Figure 4-14 shows; Figure 4-14 4.4.4 Connecting the Keyboard Take out the keyboard from the accessory kit and connect it to the keyboard interface marked “KB” at the back of the analyzer. 4.4.5 Connecting the Printer (Optional) Follow the printer’s instructions for use to connect the printer to the parallel port at the back of the analyzer.
Page 68: Starting The Analyzer
Installing Your Analyzer 4.5 Starting the Analyzer Take out the power cord from the accessory box. Plug the non-pronged end into the AC input at the back of the analyzer and the pronged end into an electrical outlet. Place the power switch at the back of the analyzer in the ON position (1) to turn on the analyzer.
Page 69: Customizing The Analyzer Software
Customizing the Analyzer Software 5.1 Introduction The BC-3000 Plus is a flexible laboratory instrument that can be tailed to your work environment. You can use the “Setup”program to customize the software options as introduced in chapters 5.2 - 5.13.
Page 70: Print
Customizing the Analyzer Software 5.2 Print The “Print” screen is where you set printing options. 5.2.1 Entering the “Print” screen Press [MENU] to enter the system menu. Figure 5-1System menu SELECT “Setup → Print” (Figure 5-1) to enter the “Print” screen（Figure 5-2）. Figure 5-2 “Print”...
Page 71 Customizing the Analyzer Software 5.2.2 Selecting printing device You can select either the built-in recorder or an external printer (if available) as the printing device, as Figure 5-3 shows. Figure 5-3 Selecting printing device If you prefer the recorder, SELECT “Recorder” from the “Device” pull-down list. If you prefer the printer, SELECT “Printer”...
Page 72 Customizing the Analyzer Software Figure 5-4 Selecting printing format for the printer If you have selected the recorder, you can choose any of the following 4 printing formats. Format1 - parameter values + histograms; Format2 – parameter values only; Format3 - parameter values + histograms; Format4 - parameter values only.
Page 73 Customizing the Analyzer Software 5.2.4 Enabling/disabling Auto print If the “Auto Print ” function is on, the analysis result will be automatically printed out once the analysis is finished. To enable (or disable) this function, SELECT “ON”(or “OFF”) from the “Auto Print”...
Page 74: Count Time
Customizing the Analyzer Software 5.3 Count time The “Count Time” screen is where you view and/or set (if you have the administrator password) the reference time for the WBC and/or RBC count portion of the measurement cycle. If the actual WBC or RBC count time (see Chapter 3.4.1 and 3.5.1) deviates from the reference time by 2 seconds or more, the analyzer will alarm you for clogging or bubbles and invalidate the results of all related parameters.
Page 75 Customizing the Analyzer Software Figure 5-8 “Count Time” screen 5.3.2 Setting Count Time 1. Enter the administrator password as instructed by Chapter 5.4.1. 2. Enter the “Count Time” screen. 3. ENTER the desired number into the ”WBC Count Time” box or ”RBC Count Time” box to set the reference WBC or RBC count time.
Page 76: Password
Customizing the Analyzer Software 5.4 Password The BC-3000 Plus classifies users into two categories: common users (default) and administrators. You need to enter the administrator password to adjust certain options such as WBC/RBC Count Time, Gain, etc. 5.4.1 Entering the administrator password Press [MENU] to enter the system menu.
Page 77 Customizing the Analyzer Software ENTER “3000” and a message box will pop up to remind you of the current password level, as Figure 5-11 shows. Figure 5-11 A message box to confirm the user level CLICK “Enter” to confirm the password and exit to the system menu. 5.4.2 Resuming the common user password Enter the “Password”...
Page 78 Customizing the Analyzer Software Table 5-1 Demographic groups Group Gender General Not specified, male or female. Not specified. Not specified. ＞ 12 years Male ＞ 12 years Woman Female ＞ 12 years Child Male or Female ＞ 28 days and ≤12 years Neonate Male or Female ≤...
Page 79 Customizing the Analyzer Software Figure 5-14 “General” screen Manufacturer-recommended ranges of all 19 parameters are available for the General, Man, Woman and Child groups. As for the Neonate group, manufacturer-recommended ranges are available for WBC, Lymph#, RBC, HGB and PLT only and the rest, if deemed necessary, can be set by the users themselves.
Page 80 Customizing the Analyzer Software Figure 5-15 “General”screen 5.4.6 Exiting the “Ref.Range” screen (e.g. the “General” group) Press [MENU] to exit to the system menu; press [MAIN] to return to the “Count” screen. If you have made any changes, a message box will pop up to ask you to save the changes, as Figure 5-16 shows.
Page 81 Customizing the Analyzer Software At the “General” screen (or the screen of any other group), you can press [PRINT] to print out the displayed limits. At the “General” screen (or the screen of any other group), you can press [DEL] to resume the manufacturer-recommended settings. 5-13...
Page 82: Transmission
Customizing the Analyzer Software 5.5 Transmission The “Transmission” screen is where you set communication parameters. 5.5.1 Entering the “Transmission” screen Press [MENU] to enter the system menu. Figure 5-17 System menu SELECT “Setup→Transmission” (Figure 5-17) to enter the “Transmission” screen (Figure 5-18).
Page 83 Customizing the Analyzer Software 5.5.2 Selecting baud rate Five baud rate options are available: “19200”, “9600”(default), “4800”, “2400” and “1200”. To select the desired baud rate, SELECT the desired rate from the “Baud Rate”pull-down list, as Figure 5-19 shows. Figure 5-19 Selecting baud rate 5.5.3 Selecting parity Three parity options are available: “Odd”, “Even”...
Page 84 Customizing the Analyzer Software Figure 5-20 Selecting parity check 5.5.4 Enabling/disabling handshake If the “Handshake”function is enabled, the analyzer will send a handshake signal to the host and wait for the response. If the host does not respond in 8 seconds, the analyzer will abort the transmission and alarm you for a transmission error.
Page 85 Customizing the Analyzer Software Figure 5-21 Enabling/disabling handshake 5.5.5 Enabling/disabling auto transmission When the auto transmission function is enabled, the analyzer will automatically transmit the analysis result to the host once the analysis is finished. To enable (or disable) the auto transmission function, SELECT ”ON”...
Page 86 Customizing the Analyzer Software 5.5.6 Exiting the “Transmission” screen Press [MENU] to exit to the system menu or [MAIN] to exit to the “Count” screen, and the changes will be saved automatically. 5-18...
Page 87: Setting System Time (Date & Time)
Customizing the Analyzer Software 5.6 Setting system time (Date & Time) The “Date & Time” screen is where you set the system date and time. 5.6.1 Entering “Date & Time” screen Press [MENU] to enter the system menu. Figure 5-23 System menu SELECT “Setup→Date &...
Page 88 Customizing the Analyzer Software 5.6.2 Selecting date format Three date formats are available: “YYYY-MM-DD”, “MM-DD-YYYY” and “DD-MM-YYYY”. To select the desired format, SELECT the desired format from the “Format”pull-down list, as Figure 5-25 shows. Figure 5-25 Selecting date format 5.6.3 Setting system time Respectively ENTER desired numbers into the ”...
Page 89: Gain
Customizing the Analyzer Software 5.7 Gain The ”Gain” screen is where you view and/or set (if you have the administrator password) gains of the “WBC (Whole Blood)”, “WBC (Predilute)”, “RBC” and “HGB” gains. 5.7.1 Entering the “Gain” screen Press [MENU] to enter the system menu. Figure 5-26 System menu SELECT “Setup→Gain”(Figure 5-26) shows to enter the ”Gain”...
Page 90 Customizing the Analyzer Software 5.7.2 Setting WBC channel gain You can adjust the shape of the WBC histogram by adjusting the gain of the WBC channel. When WBC histograms of most samples are similar to Figure 5-28, it implies too small a WBC gain and you need to increase the gain appropriately.
Page 91 Customizing the Analyzer Software Figure 5-30 Setting WBC（Whole）gain Figure 5-31 Setting WBC（Predilute）gain 5.7.3 Setting the RBC gain If the MCV results of most calibration or QC runs deviate from the expected result by 6%, you need to follow the rule below to change the RBC gain to adjust the MCV results. Assume the expected MCV result is 90.0fL and the obtained MCV result is 82.0fL.
Page 92 Customizing the Analyzer Software ExpectedMC × × ＝ ActualMCV 1. Enter the administrator password as introduced in Chapter 5.4.1. 2. Enter the “Gain” screen and ENTER a number into the ”RBC” box, as Figure 5-32 shows, so that RBC “Factor” is as close to 109.8% as possible. Figure 5-32 Setting RBC gain 5.7.4 Setting HGB channel gain You can adjust the HGB blank voltage by adjusting the HGB gain.
Page 93 Customizing the Analyzer Software Figure 5-33 Setting HGB gain 5.7.5 Exiting the “Gain” screen Press [MENU] or [MAIN] to exit the ”Gain” screen and a message box will pop up to ask you save the changes, as Figure 5-34 shows. Figure 5-34 A message box to confirm the changes CLICK “Enter”...
Page 94: Auto Clean Time
Customizing the Analyzer Software 5.8 Auto Clean Time The “Auto Clean Time” screen is where you set the interval for auto cleaning of the fluidic lines. The valid interval is 2 - 24 hours and the default interval is 4 hours. To set the interval, Press [MENU] to enter the system menu.
Page 95: Reagent Exp. Date
Customizing the Analyzer Software 5.9 Reagent Exp. Date The ”Reagent Exp. Date” screen is where you set expiration dates for the diluent, rinse and lyse. The analyzer will alarm you for expired reagents when the system time exceeds any of the three expiration dates.
Page 96 Customizing the Analyzer Software 5.9.2 Setting the expiration date ENTER the desired expiration dates into the ” Diluent”, “Rinse” and ” Lyse” boxes. For any reagent, the entered expiration date should be either the expiration date printed on the labeling or the open-container expiration date, whichever is earlier.
Page 97: Report Title （External Keyboard Required
Customizing the Analyzer Software 5.10 Report Title （external keyboard required） The “Report Title” screen is where you set the title of the report to be printed. To set the report title, Press [MENU] to enter the system menu. Figure 5-40 System menu SELECT “Setup →...
Page 98 Customizing the Analyzer Software ENTER the desired title in the ”Report Title (by recorder) ” or ” Report Title (by printer) ” box, depending on the printing device you choose to print out the report. Press [MENU] or [MAIN] to save the changes and exit to the system menu or the system menu or the “Count”...
Page 99: Parameter Units
Customizing the Analyzer Software 5.11 Parameter Units The “Parameter Units” screen is where you view and/or set (if you have the administrator password) the reporting units of the parameters. See Table 5-2 for the available units for every parameter groups. Note that if you choose g/L or g/dL for the HGB/MCHC group, the MCH unit will automatically change to pg and its reporting format will be ***.*;...
Page 100 Customizing the Analyzer Software **.* **.* None Default .*** Default *.** mL/L 5.11.1 Entering the “Parameter Units” screen and viewing the settings Press [MENU] to enter the system menu. Figure 5-42 System menu SELECT ”Setup→ Parameter Units” (Figure 5-42), to enter the “Parameter Units” screen (Figure 5-43 ).
Page 101 Customizing the Analyzer Software 5.11.2 Setting reporting units 1. Enter the administrator password as instructed in Chapter 5.4.1. 2. Enter the “Parameter Units” screen. 3. SELECT the desired unit from the corresponding pull-down list (e.g. RBC in Figure 5-44). Figure 5-44 Selecting a unit for RBC 5.11.3 Exiting the “Parameter Units”...
Page 102: Other
Customizing the Analyzer Software 5.12 Other The “Other” screen is where you define miscellaneous system settings. 5.12.1 Entering the “Other” screen Press [MENU] to enter the system menu. Figure 5-45 System menu SELECT “Setup→Other”(Figure 5-45) to enter the ”Other” screen (Figure 5-46). Figure 5-46 “Other”...
Page 103 Customizing the Analyzer Software 5.12.2 Muting the Beeper The analyzer beeps when an error is present. You can mute the beeper by pressing any key (except for the aspirate key), as long as the ”Any key to mute” function is enabled. To enable (or disable) the function, SELECT ”ON”...
Page 104 Customizing the Analyzer Software Figure 5-48 Selecting LCD contrast 5.12.4 Setting alarm time for error messages The alarm time of the errors listed in Table 5-3 can be set to 2 - 120 seconds. When the pre-set alarm times out, both the alarm sound and the corresponding error message will disappear.
Page 105 Customizing the Analyzer Software Figure 5-49 Selecting alarm time 5.12.5 Reminder of Predilute mode If you have enabled the “Reminder of Predilute mode” function and selected the Predilute mode, the analyzer will ask you to confirm the selection. To enable (or disable) the “Reminder of Predilute mode” function, SELECT “ON”(or “OFF”) from the “Reminder of Predilute mode”...
Page 106 Customizing the Analyzer Software Figure 5-51 A message box to confirm the predilute mode 5.12.6 Selecting how to enter sample information If you have entered the administrator password, you can select in which way to enter the sample information, “ID only” (to enter the sample ID only) or “All Info.”(to enter all the sample information), by selecting “ID Only”...
Page 107: Operating Your Analyzer
Operating Your Analyzer 6.1 Introduction This chapter provides step-by-step procedures for operating your analyzer on a daily basis. A flow chart indicating the common daily operating process is presented below. Initial Checks Power on Daily Quality Control Sample Collection and Handling Whole Blood Mode？...
Page 108: Initial Checks
Operating Your Analyzer 6.2 Initial Checks Perform the following checks before turning on the analyzer. 1. Checking the waste container; 2. Check and make sure the container is empty. Samples, controls, calibrators and waste are potentially infectious. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory.
Page 109: Power-On
Operating Your Analyzer 6.3 Power-on Place the power switch at the back of the analyzer in the ON position (1) to turn on the analyzer. The power indicator light will be illuminated and the screen will display “Initializing…“. The analyzer will sequentially initialize the file, hardware and fluidic systems and the whole initializing process lasts 3 to 5 minutes, depending on how the analyzer was previously shut down.
Page 110: Daily Quality Control
Operating Your Analyzer 6.4 Daily Quality Control Before running any samples, run the controls. See Chapter 8 Using the QC Programs for details.
Page 111: Sample Collection And Handling
Operating Your Analyzer 6.5 Sample Collection and Handling Samples, controls, calibrators and waste are potentially infectious. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. Avoid direct contact with blood samples. Do not re-use disposable products.
Page 112 Operating Your Analyzer 6.5.2 Prediluted Samples 1. Collect and handle the prediluted sample as follows; 2. Press [MENU] and SELECT ”Mode” to enter the ”Mode” screen; 3. SELECT “Predilute” from the “Sample Mode” pull-down list; 4. Press [MENU] and SELECT ”Count” to enter the ”Count” screen; 5.
Page 113 Operating Your Analyzer Be sure to keep dust from the prepared diluent. After mixing the capillary sample with the diluent, be sure to wait 3 minutes before running the sample. Be sure to run the prediluted samples within 30 minutes after the mixing. Be sure to mix any sample that has been prepared for a while before running Be sure to evaluate predilute stability based on your laboratory’s sample population and sample collection techniques or methods.
Page 114: Running Whole Blood Samples
Operating Your Analyzer 6.6 Running Whole Blood Samples Press [MENU] and SELECT ”Mode” to enter the ”Sample Mode” screen, as Figure 6-4 shows. Figure 6-3 System menu SELECT “Whole Blood” from the “Sample Mode” pull-down list. Figure 6-4 “Sample Mode” Screen Press [MENU] and SELECT ”Count”...
Page 115 Operating Your Analyzer Figure 6-5 “Count” Screen Be sure to select proper reference range as instructed in Chapter 5.5 before running the samples. Otherwise, the obtained results may be erroneously flagged. When switching from the predilute mode to the whole blood mode, the analyzer will automatically wash the fluidic system.
Page 116 Operating Your Analyzer Figure 6-6 Entering all sample information Entering sample ID ENTER the ID number in the “ID” box. If you have the bar-code scanner installed, you can simple scan the sample ID into the analyzer. Selecting patient gender SELECT the desired item from the “...
Page 117 Operating Your Analyzer Entering the patient age This analyzer provides three ways for you to enter the patient age – in years, in months and in days. The first way is designed for the adult or pediatric patients no younger than one year; the second for the infant patients one month to one year;...
Page 118 Operating Your Analyzer Entering the names of the sender, tester and reviewer To enter the name of the person who sent the sample for analysis, ENTER the name into the “Sender” box or SELECT the desired name from the “Sender” pull-down list (if there are previously saved names in the list, as Figure 6-9 shows) ;...
Page 119 Operating Your Analyzer “Cancel” button If you do not want to save the entered information, CLICK the “Cancel” button to return to the ”Count” screen without saving the changes. ID Only At the ”Count” screen, press [ID] and an ID window will pop up, as Figure 6-10 shows. Figure 6-10 ID window ENTER the sample ID into the ID box and press [ENTER] to save the changes and close the window.
Page 120 Operating Your Analyzer Be sure to keep the sample probe tip away from the tube bottom, otherwise the aspiration volume may be inaccurate. When the aspiration is done, remove the sample tube only when the sample probe is out of the tube. 1.
Page 121 Operating Your Analyzer Parameter flags If the analysis result is followed by an ”H” or “L”, it means the analysis result has exceeded the upper or lower limit of the reference range. If you see *** as opposed to the result, it means the result is either unreliable or out of the operating range.
Page 122 Operating Your Analyzer When the PLT value is less than 100 × 10 / L, a manual count by the microscope is recommended. Adjusting histograms manually If you are not satisfied with the obtained histograms, you can adjust them manually, provided you have the administrator password.
Page 123: Running Prediluted Samples
Operating Your Analyzer 6.7 Running Prediluted Samples Press [MENU] and SELECT ”Sample Mode” (Figure 6-11) to enter the ”Sample Mode” (Figure6-12) screen. Figure 6-11 System menu SELECT “Predilute” from the “Sample Mode” pull-down list. Figure6-12 Selecting Predilute mode 6-17...
Page 124 Operating Your Analyzer Press [MENU] and SELECT ”Count” to enter the ”Count” screen,as Figure6-13 shows. Figure6-13 “Count” screen Be sure to select a proper reference range as instructed in Chapter 5.5 before running the samples. Otherwise, the obtained results may be erroneously flagged.
Page 125 Operating Your Analyzer Figure6-14 Entering all sample information Entering sample ID ENTER the ID number in the “ID” box, or if you have the bar-code scanner installed, you can simply scan the sample ID into the analyzer. Selecting patient gender SELECT the desired item from the “Gender”...
Page 126 Operating Your Analyzer Entering the patient name ENTER the patient name into the“Name”box. Entering the patient age This analyzer provides three ways for you to enter the patient age –in years, in months and in days. The first way is designed for the adult or pediatric patients no younger than one year; the second for the infant patients one month to one year;...
Page 127 Operating Your Analyzer Figure6-16 How to select department name from the pull-down list Entering the names of the sender, tester and reviewer To enter the name of the person who sent the sample for analysis, enter the name into the “Sender”...
Page 128 Operating Your Analyzer “Enter” button When you have finished entering the all the interested sample information, CLICK the “Enter” button to save the changes and return to the “Count” screen. “Cancel” button If you do not want to save the entered information, CLICK the “Cancel” button to return to the ”Count”...
Page 129 Operating Your Analyzer The sample probe tip is sharp and may contain biohazardous materials. Exercise caution to avoid contact with the probe when working around it. Do not re-use such disposable product as collection tubes, test tubes, capillary tubes, etc. Be sure to keep the sample probe tip away from the tube bottom, otherwise the aspiration volume may be inaccurate.
Page 130 Operating Your Analyzer 6.7.3 Special Functions Automatic saving of analysis results This analyzer automatically saves maximum 35,000 sample results. When the maximum number has been reached, the newest result will overwrite the oldest. Parameter flags If the analysis result is followed by an ”H” or “L”, it means the analysis result has exceeded the upper or lower limit of the reference range.
Page 131 Operating Your Analyzer presence of large platelet, platelet coagulation, small red blood cell, cell debris or fibrin. ：indicates excessive small PLTs. ：indicates excessive large PLTs. When the PLT value is less than 100 × 10 / L, a manual count by the microscope is recommended.
Page 132: Shutdown
Operating Your Analyzer 6.8 Shutdown Perform the “Shutdown”procedure to shut down the analyzer daily. To ensure stable analyzer performance and accurate analysis results, be sure to perform the “Shutdown”procedure to shut down the analyzer after it has been running continuously for 24 hours. Be sure to shut down the analyzer strictly as instructed below.
Page 133 Operating Your Analyzer 3. CLICK “Enter”and a window will pop up to instruct you how to shut down the analyzer, as Figure6-21shows; Figure6-21 Shutdown window The sample probe tip is sharp and may contain biohazardous materials. Exercise caution to avoid contact with the probe when working around it. The reagents are irritating to eyes, skin and diaphragm.
Page 134 Operating Your Analyzer Samples, controls, calibrators and waste are potentially infectious. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. Be sure to dispose of reagents, waste, samples, consumables, etc. according to government regulations.
Page 135: Reviewing Sample Results
Reviewing Sample Results 7.1 Introduction The analyzer automatically saves analysis results. Totally 35,000 results can be saved. You can either browse all the saved sample results in general (see “Browsing All Sample Results”) or search for the results of a particular sample or samples (see “Searching for Interested Sample Results”).
Page 136: Browsing All Sample Results
Reviewing Sample Results 7.2 Browsing All Sample Results To browse all the saved sample results, you can choose either of the following modes: The “Sample Table Review” mode. In this mode, the sample results are presented in a columnar fashion without histograms (namely you can only see the parameter values). One screen displays maximum 6 sample results.
Page 137 Reviewing Sample Results Figure7-2 “Sample Table”screen Browsing sample results Press [←] or [→] to browse the preceding or following sample result; press [PgUp] or [PgDn] to browse the preceding or following screen. Switching to the “Sample Histogram Review” mode If you are interested in reviewing the histograms of the current sample result, press [6] to switch to the ”Sample Histogram Review”...
Page 138 Reviewing Sample Results Jumping to a sample result with known sample ID Press [2] and a “Find” window will pop up, as Figure7-4 shows. Figure7-4 “Find”window ENTER the sample ID into the “ID” box and press [↑] to search backward or [↓] to search forward.
Page 139 Reviewing Sample Results Figure7-6 Selecting a sample result Press [ENTER] again to deselect the sample result. Once the sample is deselected, the “*” will disappear, as sample “75” in Figure7-7 shows. Figure7-7 Deselecting a sample result Selecting/deselecting multiple sample results Example1：To select the sample results of locations 1 –...
Page 140 Reviewing Sample Results Figure7-8 Entering the “Select” window 2. ENTER the start position (“1”) into the “Start” box; 3. ENTER the start position (“5”) into the “End” box; 4. CLICK “Select” and the lower left corner of the “Select” window will display “Results selected”, as Figure7-9 shows;...
Page 141 Reviewing Sample Results Figure7-10 Reviewing the selected results Example2：To deselect the sample results of locations 1 – 5 (sample ID: 75, 77, 78, 84, 95 in Figure7-10), follow the procedure below to do so: 1. Enter the start and end positions as instructed in steps 1 – 3 of Example1; 2.
Page 142 Reviewing Sample Results will disappear, as Figure7-12 shows. Figure7-12 Reviewing the deselected results Example3: To select the sample results of locations 1 to 5 and 7 to 8, follow the procedure below to do so: 1. Select the sample results of locations 1 to 3 as instructed in steps 1 to 5 of Example1; 2.
Page 143 Reviewing Sample Results Example4：To deselect the sample results of locations 1 to 5 and 7 to 8, follow the procedure below to do so: 1. Deselect the sample results of locations 1 to 3 as instructed in steps 1 to 3 of Example2; 2.
Page 144 Reviewing Sample Results To transmit the selected sample results to a host, CLICK “Selected”；to transmit all the sample results, CLICK “All”；to stop a transmission, CLICK “Stop”; to return to the “Sample Table Review”screen, CLICK “Quit”. Deleting sample results (if configured and administrator passwored entered) Deleting some sample results Select the sample results you want to delete and press [DEL].
Page 145 Reviewing Sample Results Figure7-18 A Print message box Calculating reproducibility This analyzer provides three reproducibility indices Mean,SD （Standard Deviation） and CV% （Coefficient of Variation）. ∑ ＝ ∑ − Mean − × Mean Where n represents how many sample results are selected and X is the result of the i analysis.
Page 146 Reviewing Sample Results Figure7-19 System menu SELECT “Review → Sample Review → Sample Histogram Review” (Figure7-19) to enter the “Sample Histogram Review” screen (Figure7-20). The sample information will be displayed at the top of the screen, followed by the parameter values and histograms. The “Location/Total”...
Page 147 Reviewing Sample Results Histogram Review” mode, press [6] again. Jumping to a sample result with known location Press [1] and a “Goto” window will pop up, as Figure7-21 shows. Figure7-21 “Goto” window ENTER the location into the “Location” box and press [ENTER] to jump to the desired sample result.
Page 148 Reviewing Sample Results Entering the patient name ENTER the patient name into the “Name” box. Entering the patient age This analyzer provides three ways for you to enter the patient age – in years, in months and in days. The first way is designed for the adult or pediatric patients no younger than one year; the second for the infant patients one month to one year;...
Page 149 Reviewing Sample Results “Enter” button When you have finished entering the all the interested sample information, CLICK the “Enter” button (or press [F4] of the external keyboard) to save the changes and return to the “Sample Histogram Review” screen. “Cancel” button If you do not want to save the entered information, CLICK the “Cancel”...
Page 150 Reviewing Sample Results 2. Press [↑] or [↓] to select the WBC histogram; 3. Press [3] to select the third discriminator, as Figure7-24; Figure7-24 Adjusting discriminator (1) 4. Press [←] to move the third discriminator to 100fL, as Figure7-25 shows; Figure7-25 Adjusting discriminator (2) 5.
Page 151: Searching For Interested Sample Results
Reviewing Sample Results 7.3 Searching for Interested Sample Results 7.3.1 Starting a search At the “Sample Table Review” screen, press [F1] of the external keyboard to enter the “Search”window, as Figure7-27 shows. Figure7-27 “Search” window To include a search condition, press [↑] or [↓] to move the cursor to the desired condition and press [ENTER] to tick the condition, as Figure7-28 shows.
Page 152 Reviewing Sample Results in case you are not aware of the patient gender. Entering the department name You can either directly ENTER the name of the department, from which the sample came, into the “Department” box or SELECT the desired department from the “Department” pull-down list (if there are previously saved departments in the list).
Page 153 Reviewing Sample Results 7.3.2 Reviewing Search Result in the “Search Table Review” Mode For every search, the analyzer can display maximum 500 matches. The matches will be deleted if you have run another sample (including background check), or deleted a sample result, or restarted the analyzer after the search.
Page 154 Reviewing Sample Results Figure7-31 “Search Table Review” screen Browsing sample results Press [←] or [→] to browse the preceding or following sample result; press [PgUp] or [PgDn] to browse the preceding or following screen. Switching to the “Search Histogram Review” mode If you are interested in reviewing the histograms of the current sample result, press [6] to switch to the ”Sample Histogram Review”...
Page 155 Reviewing Sample Results Selecting/deselecting sample results You can select certain interested samples for transmission or printing. Selecting/deselecting a sample result Press [←] or [→] to move the cursor to the interested sample result and press [ENTER] to select it. The selected sample result will be marked with a “*”, as sample “75” in Figure7-33 shows.
Page 156 Reviewing Sample Results Figure7-34 Deselecting a patient result Selecting/deselecting multiple sample results Example 6：To select the sample results of locations 1 – 5 (sample ID: 75, 77, 78, 84, 95, 106 in Figure7-35), follow the procedure below to do so: 1.
Page 157 Reviewing Sample Results 3. ENTER the start position (“5”) into the “End” box; 4. CLICK “Select” and the lower left corner of the “Select” window will display “Results selected”, as Figure7-36 shows; Figure7-36 Selecting sample results of locations 1- 5 5.
Page 158 Reviewing Sample Results Example 7：To deselect the sample results of locations 1 – 5 (sample ID: 75, 77, 78, 84, 95, 106 in Figure7-38), follow the procedure below to do so: 1. Enter the start and end positions as instructed in steps 1 – 3 of Example 6; 2.
Page 159 Reviewing Sample Results Example 8: To select the sample results of locations 1 to 3 and 5 to 6, follow the procedure below to do so: 1. Select the sample results of locations 1 to 3 as instructed in steps 1 to 5 of Example 6; 2.
Page 160 Reviewing Sample Results Figure7-41 Reviewing the deselected results Printing sample results Select the sample results you want to print and press [PRINT]. A message box will pop up to ask you to confirm the printing, as Figure7-42 shows. CLICK “Enter” to print out all the selected results;...
Page 161 Reviewing Sample Results ∑ − Mean − × Mean Where n represents how many sample results are selected and X is the result of the i analysis. To check the reproducibility of the selected sample results, select at least three sample results and press [7] to view the reproducibility.
Page 162 Reviewing Sample Results SELECT “Review → Search Review → Search Histogram Review” (Figure7-43) to enter the “Search Histogram Review” screen (Figure7-44). The sample information will be displayed at the top of the screen, followed by the parameter values and histograms. The “Location/Total”...
Page 163 Reviewing Sample Results Figure7-45 “Goto” window ENTER the location into the “Location” box and press [ENTER] to jump to the desired sample result. Editing sample information Press [F1] to edit the sample information, Figure7-46 shows. Figure7-46 Editing sample information You cannot edit the sample ID of an analyzed sample. Selecting patient gender SELECT the desired item from the “Gender”...
Page 164 Reviewing Sample Results Entering the patient name ENTER the patient name into the “Name” box. Entering the patient age This analyzer provides three ways for you to enter the patient age –in years, in months and in days. The first way is designed for the adult or pediatric patients no younger than one year; the second for the infant patients one month to one year;...
Page 165 Reviewing Sample Results “Enter” button When you have finished entering all the interested sample information, CLICK the “Enter” button (or press [F4] of the external keyboard) to save the changes and return to the “Search Histogram Review” screen. “Cancel” button If you do not want to save the entered information, CLICK the “Cancel”...
Page 166 Reviewing Sample Results 2. Press [↑] or [↓] to select the WBC histogram; 3. Press [3] to select the third discriminator, as Figure7-48 shows; Figure7-48 Adjusting discriminator (1) 4. Press [←] to move the third discriminator to 100fL, as Figure7-49 shows; Figure7-49 Adjusting discriminator (2) 5.
Page 167: Using The Qc Programs
QC files. The QC files calculate the mean, standard deviation and coefficient of variation for each selected parameter. The instrument-calculated means of these ten runs should be within the expected ranges published by the manufacturer. The BC-3000 Plus provides 4 QC programs: L-J Analysis, X Analysis, X -R Analysis and X-B Analysis.
Page 168: L-J Analysis" Program
Using the QC Programs 8.2 “L-J Analysis” Program Using the “L-J Analysis” program, you can provide quality control for maximum 12 parameters. The analyzer provides 9 QC files for you to save QC settings and results. Every QC file can save results of maximum 31 QC runs. When the saved QC results have reached the maximum number, the newest result will overwrite the oldest.
Page 169 Using the QC Programs Figure8-2 “L-J Edit” screen If there are saved L-J results and settings, you need to delete them first. Press [DEL] and a message box will pop up to confirm the deletion, as Figure8-3 shows. Figure8-3 A message box to confirm the deletion CLICK “Enter”...
Page 170 Using the QC Programs Refer to the instructions of use of the control for information on the lot number, expiration date, open-vial stability days, expected results and limits. The entered expiration date should be either the expiration date printed on the labeling or the open-vial expiration date, whichever is earlier.
Page 171 Using the QC Programs Figure8-5 An“Invalid date”message box If all the entries are correct, a message box will pop up to remind you to save the changes, as Figure8-6 shows. CLICK “Enter” to save the changes and exit to the system menu (or the “Count”...
Page 172 Using the QC Programs Figure 8-7 “L-J Count”screen Be sure to use the Mindray - specified controls. Using controls other than the specified will lead to misleading results. Refer to the instructions of use of the controls for how to store and use the controls.
Page 173 Using the QC Programs When switching from the Predilute mode to the Whole Blood mode, the analyzer will automatically flush the fluidic system. Be sure to keep the sample probe tip away from the tube bottom, otherwise the aspiration volume may be inaccurate. When the aspiration is done, remove the sample tube only when the sample probe is out of the tube.
Page 174 Using the QC Programs Deleting L-J results To delete the current result, press [DEL] and a message box will pop up, as Figure8-8 shows. CLCIK “Enter” to confirm the deletion; CLICK “Cancel” to abort the deletion. Figure8-8 A message box to confirm the deletion Printing L-J results Press [PRINT] to print out the current QC result by the printer.
Page 175 Using the QC Programs SELECT “Quality Control→ L-J Analysis→ L-J Graph→ File 1”(Figure8-9) to enter the “L-J Graph” screen (Figure 8-10). Figure 8-10 “L-J Graph”screen 1 The 12 parameters are displayed on three screens, 4 parameters on every screen, as Figure 8-10 to Figure 8-12 show.
Page 176 Using the QC Programs Figure 8-11“L-J Graph”screen 2 Figure 8-12“L-J Graph”screen 3 For every parameter，the three numbers to the right of the L-J graph are defined and calculated as follows: Mean – the average of the saved QC runs; SD – Standard Deviation; CV% –...
Page 177 If you see any points fallen outside the control range, do the following steps until the problem is solved. If all the steps have failed, contanct Mindray customer service department or your local distributor for assistance.
Page 178 Using the QC Programs Browsing results of L-J analyses Press [↑] or [↓] to review the preceding or following screen; press [←] or [→] to review the preceding or following result. The parameter value of the current point (the one the cursor is located at) is displayed below the parameter box.
Page 179 Using the QC Programs Figure 8-14 “L-J Table”screen Browsing results of L-J analyses Press [PgUp] or [PgDn] to review the preceding or following screen. Deleting results of L-J analyses Press [DLE] and a message box will pop up to ask you whether to delete all the QC results saved in this file, as Figure8-15 shows.
Page 180 Using the QC Programs Figure8-16 A message box to confirm the transmission Printing results of L-J analyses Press [PRINT] to print out all the L-J analysis results. Exiting the “L-J Table” screen Press [MENU] to exit to the system menu, or press [MAIN] to exit to the “Count” screen. 8-14...
Page 181: X Analysis " Program
Using the QC Programs 8.3 “ X Analysis ” Program Using the “ X Analysis” program, you can provide quality control for maximum 12 parameters. The analyzer provides 9 QC files for you to save QC settings and results. Every QC file can save maximum 31 QC run results.
Page 182 Using the QC Programs Figure 8-18 “ X Edit” screen If there are saved X analysis results and settings, you need to delete them first. Press [DEL] and a message box will pop up to confirm the deletion, as shows Figure8-19 shows. Figure8-19 A message box to confirm the deletion CLICK “Enter”...
Page 183 Using the QC Programs Refer to the instructions of use of the control for information on the lot number, expiration date, open-vial stability days, expected results and limits. The entered expiration date should be either the expiration date printed on the labeling or the open-vial expiration date, whichever is earlier.
Page 184 Using the QC Programs saved only when both the expected result and limit are valid. Figure8-21 An“Invalid date”message box If all the entries are correct, a message box will pop up to remind you to save the changes, as Figure 8-22 shows. CLICK “Enter” to save the changes and exit to the system menu (or the “Count”...
Page 185 Using the QC Programs Figure8-23 “ X Count”screen Be sure to use the Mindray - specified controls. Using controls other than the specified will lead to misleading results. Refer to the instructions of use of the controls for how to store and use the controls.
Page 186 Using the QC Programs When switching from the Predilute mode to the Whole Blood mode, the analyzer will automatically flush the fluidic system. Be sure to keep the sample probe tip away from the tube bottom, otherwise the aspiration volume may be inaccurate. When the aspiration is done, remove the sample tube only when the sample probe is out of the tube.
Page 187 Using the QC Programs If the analyzer detects WBC/RBC clogging or bubbles during the analysis, the corresponding error messages will be displayed in the upper left corner of the screen and the results of all the related parameters will be invalidated. See Chapter 11 Troubleshooting for solutions.
Page 188 Using the QC Programs 8.3.3 Reviewing X Analysis Results You can review the X analysis results in either the “ X Graph” mode or “ X Table” mode. “ X Graph” mode Entering the “ X Graph” screen Press [MENU] to enter the system menu. Figure8-26 System menu SELECT “Quality Control→...
Page 189 Using the QC Programs The 12 parameters are displayed on three screens, 4 parameters on every screen, as Figure8-27 to Figure 8-29 show. The saved X analysis results are sequentially displayed in the X graph, the latest on the utmost left (No.1). The X graph can be interpreted as follows: The x-coordinate represents the number of the X analyses performed;...
Page 190 Using the QC Programs Figure 8-29 “ X Graph”screen 3 For every parameter，the three numbers to the right of the X graph are defined and calculated as follows: Mean – the average of the saved X analyses; SD – Standard Deviation; CV% –...
Page 191 The “□” points represents non-numeric parameter values (*). If you see any points fallen outside the control range, do the following steps until the problem is solved. If all the steps have failed, contact Mindray customer service departmentor your local distributor for assistance.
Page 192 Using the QC Programs Figure 8-30 System menu SELECT “Quality Control →Analysis →Table→ File 1” (Figure 8-30) to enter the “ X Table” screen (Figure8-31). Every screen displays 5 results. The parameter value fallen outside the expected range will be flagged “H” (higher than the upper limit) or “L” (lower than the lower limit).
Page 193 Using the QC Programs saved in this file, as Figure8-32 shows. CLICK “Enter” to confirm the deletion; CLICK “Cancel” to abort the deletion. Figure8-32 A message box to confirm the deletion Printing QC results Press [PRINT] to print out all the QC results saved in this file. Exiting the “...
Page 194: X -R Analysis" Program
Using the QC Programs 8.4 “ X -R Analysis” program Using the “ X -R Analysis” program, you can provide quality control for maximum 12 parameters. The analyzer provides 9 QC files for you to save QC settings and results. Every QC file can save maximum 31 QC run results.
Page 195 Using the QC Programs Figure8-34 “ X -R Edit” screen If there are saved QC results and settings, you need to delete them first. Press [DEL] and a message box will pop up to confirm the deletion, as Figure8-35 shows. Figure8-35 A message box to confirm the deletion CLICK “Enter”...
Page 196 Using the QC Programs Refer to the instructions of use of the control for information on the lot number, expiration date, open-vial stability days, expected results and limits. The entered expiration date should be either the expiration date printed on the labeling or the open-vial expiration date, whichever is earlier.
Page 197 Count → File 1” to enter the “ X -R Count” screen (Figure8-38). Figure8-38 “ X -R Count”screen Be sure to use the Mindray - specified controls. Using controls other than the specified will lead to misleading results. Refer to the instructions of use of the controls for how to store and use the controls.
Page 198 Using the QC Programs Samples, controls, calibrators and waste are potentially infectious. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. The sample probe tip is sharp and may contain biohazardous materials. Exercise caution to avoid contact with the probe when working around it.
Page 199 Using the QC Programs 5. CLICK “Enter” to save the result and the “NO./Total” in the upper left corner of the screen will automatically increase by 1; CLICK “Cancel” to abort the result; Figure8-39 A message to confirm the validity of the QC run 6.
Page 200 Using the QC Programs Figure8-40 A message box to confirm the deletion Printing results of X -R analyses Press [PRINT] to print out the current X -R analysis result by the printer. Exiting the “ X -R Count” screen Press [MENU] to exit to the system menu, or press [MAIN] to exit to the “Count” screen. 8.4.3 Reviewing the X -R Analysis Results You can review the X －R analysis results in either the “...
Page 201 Using the QC Programs Figure8-42“ X －R Graph”screen Every screen displays the X and R graphs of one parameter. The X graph can be interpreted as follows: The x-coordinate represents the number of the X -R analyses performed; the y-coordinate represents the results of the X -R analyses; For every parameter, its X graph can display maximum 31 points;...
Page 202 The “□” points represents non-numeric parameter values (*). If you see any points fallen outside the control range, do the following steps until the problem is solved. If all the steps have failed, contact Mindray customer service department or your local distributor for assistance.
Page 203 Using the QC Programs “ X -R Table” mode Entering the “ X -R Table” mode Press [MENU] to enter the system menu. Figure8-43 System menu SELECT “Quality Control→ X -R Analysis → X -R Table → File 1” (Figure8-43) shows to enter the “...
Page 204 Using the QC Programs Deleting results of X -R analyses Press [DLE] and a message box will pop up to ask you whether to delete all the QC results saved in this file, as Figure 8-45 shows. CLICK “Enter” to confirm the deletion; CLICK “Cancel”...
Page 205: X-B Analysis" Program
Using the QC Programs 8.5 “X-B Analysis” Program The X-B analysis is a weighted moving average analysis that uses values obtained from patient samples. It was proposed by Brian Bull, M.D. using the 3 red cell indices, MCV, MCH and MCHC to indicate the hematology instrument performance. Effective use of X-B requires randomization of samples and a normal cross section of patients to prevent skewing of indices.
Page 206 Using the QC Programs Figure8-47 “Limit” screen If there are saved QC results and settings, you need to delete them first. Press [DEL] and a message box will pop up to confirm the deletion, as Figure8-48 shows. Figure8-48 A message box to confirm the deletion CLICK “Enter”...
Page 207 Using the QC Programs ENTER the expected results (mean) and limits (range) respectively into the “Mean” box and “Range” boxes of the parameters to be included in the QC run. Deleting settings Press [DEL] to delete all the settings. Printing settings Press [Print] to print out all the settings.
Page 208 Using the QC Programs 8.5.2 Setting frequency of the X-B analysis The X-B analysis is performed on batches of certain number of patient samples. To determine how many samples are to be included in every batch, follow the steps below to do so. Entering the “Samples/Batch”screen Press [MENU] to enter the system menu.
Page 209 Using the QC Programs Setting Samples/Batch ENTER the desired number, which should be 20 to 200. 20 is recommended. Exiting the “Sample/Batch” screen Press [MENU] to exit to the system menu; press [MAIN] to exit to the “Count” screen. 8.5.3 Enabling/Disabling X-B Analysis Entering the “Start/Stop”screen Press [MENU] to enter the system menu.
Page 210 Using the QC Programs Figure8-54 Enabling/disabling X-B analysis Random samples are required for the X-B analysis. In case of known samples of a particular type (oncology, neonatal and so forth) that will seriously interfere with the X-B results, disable the X-B analysis. Enabling/disabling X-B analysis Press [PgUp] or [PgDn] to activate/deactivate X-B analysis.
Page 211 Using the QC Programs 8.5.4 Performing X-B Analysis Once enabled, the X-B analysis will be performed on batches of patient samples of the defined number (20 - 200). The analysis results will be displayed on the X-B graph as well as the X-B table.
Page 212 The “■”points fallen outside the upper and lower dash lines are out of the expected ranges If you see any points fallen outside the control range, do the following steps until the problem is solved. If all the steps have failed, contact Mindray customer service department or your local distributor for assistance.
Page 213 Using the QC Programs 2. Check the X-B settings for inappropriate entries; 3. Do the background check. In case of an abnormal background result, refer to Chapter 11 Troubleshooting Your Analyzer for solutions; 4. Run the controls; 5. Check if the analyzer needs to be calibrated. Browsing X-B analysis results Press [↑] or [↓] to review the preceding or following screen;...
Page 214 Using the QC Programs SELECT “Quality Control → X-B Analysis → X-B Table → File 1” (Figure8-58) to enter the “X-B Table” screen (Figure8-59). Every screen displays 5 results. The parameter value fallen outside the expected range will be flagged “H” (higher than the upper limit) or “L” (lower than the lower limit).
Page 215 Using the QC Programs Printing X-B analysis results Press [PRINT] to print out the displayed results by the printer. Exiting the “X-B Table” screen Press [MENU] to exit to the system menu; press [MAIN] to exit to the “Count” screen. 8-49...
Page 217: Using The Calibration Programs
Purpose of the calibration is to maintain system accuracy. Quality of the calibration depends on the calibration materials and reagents used. You should only use the calibrators and reagents specified by Mindray for the calibration. Be sure to store and use the calibrators and reagents as instructed by their instructions for use.
Page 218: When To Calibrate
Using the Calibration Programs 9.2 When to calibrate You should run the calibration program if It is the first time the analyzer has been used; Certain major component (s) of the analyzer has been changed; The quality control results indicate there may be a problem. All of the measured parameters must be calibrated before readings of this analyzer can be used as valid analysis results.
Page 219: How To Calibrate
9.3.1 Preparing Your Analyzer Do the following pre-calibration procedures before calibration. If problems are detected during these checks, do not attempt to calibrate the analyzer. If necessary, call Mindray customer service department or your local distributor for assistance. Check and make sure enough reagents have been prepared for the calibration. You need to start over the calibration if the reagents run out during the process.
Page 220 Using the Calibration Programs It is recommended that you create a log table for your analyzer. This log table should contain all necessary information that is pertinent to your analyzer. Suggested items that you may want to include in the log table are: Calibration date Supplier of calibrator Lot number...
Page 221 Using the Calibration Programs Figure9-2 “Auto Calibration” screen Selecting the count mode Press [MENU] and SELECT ”Mode” to enter the ”Mode” screen. SELECT “Whole Blood” or “Predilute” from the “Sample Mode” pull-down list. Press [MENU] and SELECT ”Count” to enter the ”Count” screen. When switching from the predilute mode to the whole blood mode, the analyzer will automatically wash the fluidic system.
Page 222 When you have finished editing the interested settings, press [ENTER] to deactivate the edit boxes. Running the calibrator Be sure to use the Mindray- specified calibrator. Using calibrator other than the specified will lead to misleading results. Refer to the instructions of use of the controls for how to store and use the calibrator.
Page 223 Using the Calibration Programs In the whole blood mode: 1. Be sure the System Status area displays “Ready“ and Count Mode area displays “Whole“; 2. Present a vial of mixed calibrator to the sample probe so that the tip is well into the tube, and press the aspirate key.
Page 224 Using the Calibration Programs Figure 9-4 How to dispense diluent 4. When the dispensing is finished, press [ENTER] to close the box; 5. Add 20µL of calibrator to the diluent and shake the tube to mix the sample. Be sure to keep dust from the prepared diluent. After mixing the calibrator with the diluent, be sure to wait 3 minutes before running it.
Page 225 Using the Calibration Programs If the analyzer detects WBC/RBC clogging or bubbles during the analysis, the corresponding error messages will be displayed in the upper left corner of the screen and the results of all the related parameters will be invalidated. See Chapter 11 Troubleshooting Your Analyzer for solutions.
Page 226 0％-75％ or 125％-9999％ will be flagged with a “*”. Other values will not be displayed. In case of an empty calibration factor, try to find out the reason and if necessary, contact Mindray customer service department or your local distributor for assistance. Verifying new calibration factors Press [MAIN] to enter the “Count”...
Page 227 At the “Count” screen, run the calibrator or a normal control material at least 5 consecutive times and calculate the mean of the results. The means should be within the expected ranges supplied by the manufacturer. If not, contact Mindray customer service department or your local distributor for assistance.
Page 228 Using the Calibration Programs 9.3.3 “Fresh Blood” Program Press [MENU] to enter the system menu. Figure 9-9 System menu SELECT “Calibration →“Fresh Blood” (Figure 9-9) to enter the “Fresh Blood” screen (Figure9-10). Figure9-10 “Fresh Blood” screen Complete the fresh blood calibration as instructed below: Selecting count mode Press [MENU] and SELECT “Mode”...
Page 229 Using the Calibration Programs Once switching from the predilute mode to the whole blood mode, the analyzer will automatically wash the fluidic system. Editing calibration settings 1. Press [1]...[5] to switch among “Sample 1” to “Sample 5” to select the sample for calibration.
Page 230 Using the Calibration Programs Figure9-12 A message box to confirm the validity CLICK “Enter” to save the results to the “Auto – fresh blood” screen; CLICK “Cancel” to abort the result. If you press [MENU] to enter the system menu before the average is obtained at the “Calculate”...
Page 231 Mindray customer service department or your local distributor. For every parameter, the analyzer will calculate the average calibration factor, which serves as the new calibration factor, only when there are at least 3 valid calibration factors（e.g.
Page 232 Run the same samples on your analyzer for the same number of times and calculate the mean (MEAN 2). The MEAN 2 should be within MEAN 1 ± 2SD. If any of the sample fails to reach the criterion, call Mindray customers service department or your local distributor. Method two: At the “Count”...
Page 233 Using the Calibration Programs Complete the manual calibration steps as introduced below. Selecting count mode Press [MENU] and SELECT “Mode” to enter the “Mode” screen. SELECT “Whole Blood” or “Predilute”from the “Sample Mode” pull-down list. Once switching from the predilute mode to the whole blood mode, the analyzer will automatically wash the fluidic system.
Page 234 Using the Calibration Programs Running the calibration material After you have selected the desired sample mode, refer to the sample handling and analysis procedures introduced in Chapter 6 Operating Your Analyzer and run the calibration material with known expected results 11 consecutive times. Checking the reproducibility When you have finished running the calibration material, enter the “Sample Table Review”...
Page 235 If the reproducibility of the calibrated parameter does not meet the requirements of Table 9-1, you must try to find out the reason and re-run the calibrator after you have solved the problem. If necessary, contact Mindray customer service department or your local distributor for assistance.
Page 236 The calculated new calibration factor should be within 75％-125％. If not, try to find out the reason and if necessary, call Mindray customer service department or your distributor for assistance. Entering the manually calculated factors Press [MENU] to enter the system menu.
Page 237 Using the Calibration Programs Press [ENTER] to activate the edit boxes as Figure 9-22 shows. Figure 9-22 Edit boxes activated ENTER the calculated calibration factor into the corresponding boxes. To correct an erroneous entry, DELETE the wrong digit and enter the correct digit. Verifying new calibration factors Press [MAIN] to enter the “Count”...
Page 238 Using the Calibration Programs Printing new calibration factors Press [PRINT] to print out the current calibration factors. Exiting the “Manual Calibration” screen Press [MENU] to exit to the system menu. A message box will pop up to confirm the new calibration factors, as Figure 9-23 shows.
Page 239: Maintaining Your Analyzer
Maintaining Your Analyzer 10.1 Introduction Routine preventive maintenance and cleaning are required to keep the BC-3000 Plus in good operating condition. Cleanliness is important in keeping your analyzer operating efficiently and accurately. The analyzer has automatic cleaning functions that are performed during normal operation.
Page 240: General Guidelines
Maintaining Your Analyzer 10.2 General Guidelines Maintenance Period Content of Maintenance Everyday If you are to use this analyzer 24 hours a day, be sure to perform the “E-Z cleanser cleaning” procedure everyday. Run the QC program everyday. See Chapter 7 Using Quality Control Programs for details.
Page 241 Maintaining Your Analyzer other error messages, Chapter Troubleshooting, for solutions. 10-3...
Page 242: Using The "Maintenance" Program
Maintaining Your Analyzer 10.3 Using the “Maintenance” Program Press [MENU] to enter the system menu. SELECT “Service → Maintenance” (Figure10-1) to enter the “Maintenance” screen (Figure10-2). Figure10-1 System menu Figure10-2 “Maintenance” screen 10-4...
Page 243 Maintaining Your Analyzer Totally 12 maintenance procedures are available at the “Maintenance” screen. Diluent Prime Rinse Prime Lyse Prime Zap Apertures Flush Apertures Probe Cleanser Cleaning E-Z Cleanser Cleaning Lyse Test Clean Baths Empty Baths Empty Tubing Wipe Block Cleaning 10.3.1 Diluent Prime Samples, controls, calibrators and waste are potentially infectious.
Page 244 Maintaining Your Analyzer At the “Maintenance” screen, SELECT “Diluent Prime” to prime the tubing with diluent and the priming progress will be displayed at the bottom of the screen, Figure10-3 shows. When the priming is done, the screen displays “Diluent Prime End”. Figure10-3 “Diluent Prime”...
Page 245 Maintaining Your Analyzer At the “Maintenance” screen, SELECT “Rinse Prime” to prime the tubing with rinse and the priming progress will be displayed at the bottom of the screen, as Figure10-4 shows. When the priming is done, the screen displays “Rinse Prime End”. Figure10-4 Rinse Priming screen 10.3.3 Lyse Prime Samples, controls, calibrators and waste are potentially infectious.
Page 246 Maintaining Your Analyzer At the “Maintenance” screen, SELECT “Lyse Prime” to prime the tubing with lyse and the priming progress will be displayed at the bottom of the screen, as Figure10-5 shows. When the priming is done, the screen will display “Lyse Prime End”. Figure10-5 Lyse priming 10.3.4 Zap Apertures You can perform the “Zap Aperture”...
Page 247 Maintaining Your Analyzer Figure10-6 Zapping aperture 10.3.5 Flush Aperture You can perform the “Flush Aperture” procedure to flush the apertures to unclog the apertures or prevent clogging. At the “Maintenance” screen, SELECT “Flush Aperture” to flush the aperture and the flushing progress will be displayed at the bottom of the screen, as Figure10-7 shows.
Page 248 Maintaining Your Analyzer 10.3.6 Probe Cleanser Cleaning Samples, controls, calibrators and waste are potentially infectious. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. The probe cleanser is corrosive. Wear proper personal protective equipment (e.g.
Page 249 Maintaining Your Analyzer 3． When the screen reminds you for the second aspiration, present the cleanser to the probe again and press [ENTER]. When you hear the beep and the sample probe is out of the bottle, remove the cleanser and the priming progress is displayed on the screen, as Figure10-9 shows.
Page 250 Maintaining Your Analyzer Figure10-10 Cleaning process To make sure this analyzer functions normally, every time the accumulated analyzed whole blood samples reach 300 or prediluted blood samples reach 150, a message box will pop up to remind you to perform the “Probe cleanser cleaning” procedure, as Figure10-11 shows. CLICK “Enter”...
Page 251 Maintaining Your Analyzer 1． At the “Maintenance” screen, SELECT “E-Z Cleanser Cleaning”; 2． Present the cleanser to the probe and press [ENTER] to aspirate the cleanser. When you hear the beep and the sample probe is out of the bottle, remove the cleanser.
Page 252 Maintaining Your Analyzer Figure10-13 E-Z cleaning Figure10-14 Draining the baths and fluidic lines 10-14...
Page 253 Maintaining Your Analyzer 10.3.8 Lyse Test Samples, controls, calibrators and waste are potentially infectious. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. In case of any abnormal WBC counts or histograms, you can perform the “Lyse Test” procedure to check whether the lyse can be dispensed properly.
Page 254 Maintaining Your Analyzer 3． Remove the screws fixing the shielding box of the bath and lift the shielding box, as Figure10-17 shows. Figure10-17 Shielding box 4． Remove the shielding box to expose the bath, as Figure10-18 shows. Figure10-18 WBC bath 10-16...
Page 255 If the lyse is still enough and the tube is well connected to the analyzer, contact the Mindray or your local distributor for repair. 10.3.9 Cleaning Baths If you suspect the baths are contaminated, follow the steps given below to perform the “Clean...
Page 256 Maintaining Your Analyzer 10.3.10 Empty Baths When at three or more of the WBC, RBC, PLT and HGB results are abnormal, you may do the “Empty Baths” procedure to find out the reason. Follow the steps below to do so: 1.
Page 257 Maintaining Your Analyzer Figure10-21 Priming the baths with diluent If there is fluid left, turn off the analyzer and call Mindray customer service department or your local distributor for assistance. 10-19...
Page 258 Maintaining Your Analyzer 10.3.11 Empty Tubing Samples, controls, calibrators and waste are potentially infectious. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. Be sure to do the “Empty Tubing” procedure before relocating the analyzer. If this analyzer is not to be used for a long time or it is to be maintained, be sure to perform the “Empty Tubing”...
Page 259 Maintaining Your Analyzer 10.3.12 Wipe Block Cleaning After being used for a long time, the bottom of the probe wipe may be contaminated by blood and the inside of wipe may also be contaminated by the dirt sucked in. So you need to clean the probe wipe regularly.
Page 260 Maintaining Your Analyzer 5. Follow the instructions displayed on the screen to place an empty cup, whose diameter should be no less than 8cm, below the sample probe. 6. Press [ENTER] to soak the wipe block with the aspirated cleanser. The soaking progress will be displayed on the screen, as Figure10-25 shows.
Page 261 Maintaining Your Analyzer Figure10-26 Washing interior of the probe wipe and sample probe Figure10-27 A “Wipe Block Clean” message box 10-23...
Page 262: Using The "System Status" Program
Maintaining Your Analyzer 10.4 Using the “System Status” Program The items displayed in the “System Status” screen reflect how the analyzer is functioning and contribute significantly to diagnosing analyzer errors. You may follow the instructions given below to check those items. Press [MENU] to enter the system menu.
Page 263 Maintaining Your Analyzer Note that you can only view the displayed status items without changing them. If any of the displayed item exceeds the given range, see Chapter 11 Troubleshooting for solutions. Press [MENU] to exit to the system menu and the screen will display “Resetting” and the system menu will pop up later.
Page 264: Using The "Valve Test" Program
Maintaining Your Analyzer 10.5 Using the “Valve Test” Program Malfunctioning valves will lead to fluidic system malfunctions. Therefore, testing the valves is a major way to remove fluidic errors. Press [MENU] to enter the system menu. SELECT “Service → Valve Test” (Figure10-30) to enter the “Valve Test”...
Page 265: Using The "System Test" Program
Maintaining Your Analyzer 10.6 Using the “System Test” Program Press [MENU] to enter the system menu. SELECT “Service → System Test” ( Figure10-32) to enter the “System Test” screen (Figure10-33), where 19 test items are available. Note that you need to enter the administrator password to test the motors. SELECT the desired item to perform the corresponding test.
Page 266: Using The "Prepare To Ship" Program
Maintaining Your Analyzer 10.7 Using the “Prepare to ship” Program Use the “Prepare to ship” program to prepare your analyzer for a prolonged period of non-use or for shipping. Press [MENU] to enter the system menu. SELECT “Service→Prepare to ship”(Figure10-34) to enter the “Prepare to Ship”...
Page 267 Maintaining Your Analyzer Figure10-36 A message box to confirm the operation 2. CLICK “Enter” to proceed with the operation. 3. The analyzer starts to drain the fluidic lines and the progress is displayed on the screen, as Figure10-37 shows. Figure10-37 Draining fluidic lines 4.
Page 268 Maintaining Your Analyzer Figure10-38 Washing the analyzer 5. When the washing is done, remove the diluent, rinse and lyse pickup tubes from the distilled water and press [ENTER] to drain the fluidic lines. 6. When the draining is done and the screen displays “You can turn off the analyzer now”, turn off the analyzer as instructed.
Page 269: Using The "Error Message" Program
Maintaining Your Analyzer 10.8 Using the “Error Message” Program The analyzer can store maximum 1,000 latest error messages. When the maximum number has reached, the latest overwrites the earliest. Press [MENU] to enter the system menu. SELECT “Service → Error Message” (Figure10-39) to enter the “Error Message”...
Page 270 Maintaining Your Analyzer For the displayed error messages, see Chapter 11 Troubleshooting Your Analyzer for solutions. Press [MENU] to exit the “Error Message” screen. 10-32...
Page 271: Calibrating Sample Probe Position
Maintaining Your Analyzer 10.9 Calibrating Sample Probe Position Samples, controls, calibrators and waste are potentially infectious. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. The sample probe tip is sharp and may contain biohazardous materials. Exercise caution to avoid contact with the probe when working around it.
Page 272 Maintaining Your Analyzer Figure10-42 3. Lift up the front panel latch as indicated in Figure10-43 and open the front panel; Figure10-43 4. SELECT “Service” →“System Test” to enter the “System Test” screen and SELECT “Elevator motor”; 5. Press [↑] to move the sample probe to its highest position, as Figure10-44 shows; 10-34...
Page 273 Maintaining Your Analyzer Figure10-44 6. Loose the retaining screw by a screwdriver, as Figure10-45 shows; Figure10-45 7. Remove the probe from the wipe block and insert the localizer into the wipe block from the bottom, as Figure10-46 shows; Figure10-46 8. Insert the probe into the wipe block until it reaches the localizer, as Figure10-47 shows; 10-35...
Page 274 Maintaining Your Analyzer Figure10-47 9. Tighten the fixing screws and put away the localizer to finish the work. 10-36...
Page 275: Replacing The Probe Wipe
Maintaining Your Analyzer 10.10 Replacing the Probe Wipe. Samples, controls, calibrators and waste are potentially infectious. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. The sample probe tip is sharp and may contain biohazardous materials. Exercise caution to avoid contact with the probe when working around it.
Page 276: Replacing The Filter Of The Vacuum Chamber
Maintaining Your Analyzer 10.11 Replacing the Filter of the Vacuum Chamber You need to replace the filter of the vacuum chamber when there is an air filter error. Follow the steps below to do so: 1. Push the right door latch in the direction indicated in Figure10-49; Figure10-49 2.
Page 277: Using The [Flush] Key
Maintaining Your Analyzer 10.12 Using the [Flush] key Press the [Flush] key to unclog the apertures when the analyzer alarms you for clogged WBC or RBC aperture. 10-39...
Page 278: Using The [Startup] Key
Maintaining Your Analyzer 10.13 Using the [Startup] key Press the [Startup] key to flush the fluidic lines and check the background. 10-40...
Page 279: Troubleshooting Your Analyzer
Troubleshooting Your Analyzer 11.1 Introduction The BC-3000 Plus continuously monitors the status of the system and displays pertinent information in the upper left corner of the “Count” screen (the Error Message area). If a problem is detected, the Error Message area displays the corresponding error message. This chapter contains information that is helpful in locating and correcting problems that may occur during operation of your analyzer.
Page 280: Errors Without Available Error Messages
Damaged pump hose or 1. Turn off the power and wipe the analyzer inside. blocked filter. analyzer dry. 2. Call Mindray customer service department or your local distributor for assistance. Recorder does not 1. Recorder paper 1. Remove the jammed paper.
Page 281: Errors Indicated By Error Messages
The analyzer can provide 41 error messages. See the tables below for the error messages and their probable causes and recommended action. If the problem still remains after you have tried the recommended solutions, contact Mindray customer service department or your local distributor.
Page 282 Chapter within the given time. 10.6. The error will be removed if the test result is normal; 2. If the problem remains, contact Mindray customer service departmentor your local distributor. Vacuum Filter The air inside the vacuum 1.
Page 283 Troubleshooting Your Analyzer as instructed by Chapter 4.4.2; 3. If the problem remains, contact Mindray customer service departmentor your local distributor. Rinse Empty No rinse or a malfunctioning 1. Check if the rinse has run out, and if level transducer.
Page 284 (poor contact, dead battery, 2. If the problem remains, contact etc.); Mindray customer service department or your local distributor. 3. Damaged real-clock chip. 10ml Motor Error 1. Pressed or blocked tubes; 1. Check if the tubes at the back of the analyzer is pressed or blocked;...
Page 285 CUP board; 3. If the problem remains, contact 5. Malfunctioning photo Mindray customer service coupler. department or your local distributor. Rotation Motor 1. Jammed sample probe;...
Page 286 Status” screen and record the “DC-DC 12V” and “DC-DC -12V” values; Shut down analyzer contact Mindray customer service department or your local distributor. 5V Power Error Something is wrong with the Enter the “Service → System power board. Status” screen and record the “5V”...
Page 287 Troubleshooting Your Analyzer “3.3V” voltage; Shut down analyzer contact Mindray customer service department or your local distributor. 56V Power Error Something is wrong with the Enter the “Service → System power board. Status” screen and record the “56V” voltage; Shut...
Page 288 HGB gain as instructed by Chapter 5.8.3 to set the voltage within 3.4 - 4.8V, preferably 4.5V; 3. If the problem remains, shut down your analyzer and contact Mindray customer service department or you local distributor. HGB Adjust HGB blank voltage within 3.2 1.
Page 289 WBC count time. Then enter the “Service → System Test” screen and test the actual WBC count time instructed confirm Chapter 10.6 difference is less than 2 seconds. If the problem remains, contact Mindray customer service department or your local distributor. 11-11...
Page 290 Chapter 4.4.2; 3. If the problem remains, adjust the WBC count time as instructed by Chapter 5.3; 4. If the problem remains, contact Mindray customer service department or your local distributor. RBC clog 1. Clogged RBC aperture;...
Page 291 RBC count time as instructed by Chapter 10.6 to confirm the difference is less than 2 seconds. If the problem remains, contact Mindray customer service department or your local distributor. RBC bubbles 1. Diluent or rinse running 1.
Page 292 Poor connection between the Check if the printer is well connected to printer and the analyzer. the analyzer. Recorder Shut down the analyzer and contact 1. Poor connection between Error Mindray customer service department. recorder analyzer; 2. Damaged recorder. 11-14...
Page 293 2. Check if the recorder paper is properly installed. If not, re-install the paper as instructed by Chapter 4.4.3; 3. If the problem remains, contact Mindray customer service department or your local distributor. Recorder too Hot Recorder head too hot.
Page 294 Troubleshooting Your Analyzer unreliable; 3. If the actual temperature is within the pre-defined range and the problem remains, contact Mindray customer service department or your distributor. 11.3.8 Other errors Error Message Possible Cause(s) Recommended Action File Error Something is wrong with the Shut down the analyzer and contact file system.
Page 295: Appendices
Appendices Index calibrator, 2-15 analyzer clog, 5-36 name, 2-1 RBC, 11-12 intended use, 2-2 WBC, 11-10 aperture control, 2-15 flush, 10-9 Coulter Principle, 3-6, 3-10 zap, 10-8 count principle, 3-6, 3-10 procedure, 6-8, 6-17 Bath definition, 3-11 clean, 10-17 formula, 7-11, 7-27 empty, 10-18 baud rate, 5-15 blank photocurrent, 3-7, 3-8...
Page 296 Appendices 3.3V Power Error, 11-8 Rotation Motor Error, 11-7 5V Power Error, 11-8 Vacuum Filter Error, 11-4 56V Power Error, 11-9 Vacuum Error, 11-3 2.5 mL&50μL Motor Error, 11-6 WBC Interrupt Error, 11-7 Ambient Tem. Abnormal, 11-15 WBC Bubbles, 11-12 Background Abnormal, 11-9 WBC Clog, 11-10 Barcord Error, 11-14...
Page 297 Appendices Ajust histograms, 6-16, 6-25 formula, 3-10 humidity, 4-3 definition, 2-2, 3-11 linearity range, 12-4 reproducibility, 12-5 ID, 5-38, 6-13 Mid# installation, 4-1 definition, 2-2 requirements, 4-2 formula, 3-8 procedure, 4-5 Mid% definition, 2-2 formula, 3-7 LCD, 2-8 definition, 2-2, 3-12 leukocyte, 2-2 granulocyte, 2-2 lymphocyte, 2-2...
Page 298 Appendices Lymph#, 2-2 probe cleanser Mid#, 2-2 definition, 2-16 Gran#, 2-2 use, 10-2, 10-10 HCT, 2-2 probe wipe MCH, 2-2 calibrate, 10-33 MCHC, 2-2 replace, 10-37 PCT, 2-2 , 6-15 Parity, 5-15 password, 5-8 Quality control definition, 2-2 Edit settings, 8-2, 8-15, 8-28, 8-39 formula, 3-12 L-J Analysis, 8-2 review, 8-8,8-18,8-34, 8-45...
Page 299 Appendices Install papers, 4-13 transmission pringting format, 5-3 data format, 12-3 rinse at count screen, 12-8 connection, 4-11 at QC Table Screen, 12-8 definition, 2-16 at review screen, 12-8 prime, 10-6 troubleshooting, 11-1 sample unpacking, 4-4 samples collection and handling, 6-5 run, 6-8, 6-17 sample probe valve...
Page 300 Appendices edit, 8-28 run, 8-31 review, 8-34 zap apertures, 10-8...
Page 301: B Specifications
Specifications B.1 Classification According to the CE classification, the BC-3000 Plus is an In Vitro Diagnostic device. B.2 Reagents M-30D DILUENT Diluent Rinse M-30R RINSE Lyse M-30CFL LYSE E-Z Cleanser （Enzyme cleanser） M-30E E-Z CLEANSER Probe Cleanser M-30P PROBE CLEANSER...
Page 302 Appendices Table 12-3 Calculated parameters Parameter Abbreviation Default Unit Lymphocyte Lymph# Mid-sized cell Mid# Granulocyte Gran# Hematocrit Mean Cell Hemoglobin Mean Cell Hemoglobin Concentration MCHC Mean Platelet Volume B.4 Sampling Features B.4.1 Sample volumes required for each analysis Whole Blood Mode (vein blood) 13 µL Prediluted Mode (capillary blood) 20 µL...
Page 303 Appendices B.5 Performance specifications B.5.1 Operating range Parameter Operating range WBC (10 0.0 - 999.9 RBC (10 0.00 - 9.99 HGB (g/L) 0 - 300 MCV (fL) 0.0 - 250.0 PLT (10 0 - 2999 B.5.2 Normal background Parameter Background result ≤...
Page 304 Appendices B.5.4 Reproducibility These reproducibility requirements applies only to the situation in which 11 normal-level controls have been run and the results of the 2 to 11 runs are used to calculate the reproducibilities. Reproducibility（CV%） Parameter Condition 7.0 - 15.0 × 10 ≤...
Page 305 Two RS-232 interfaces (maximum transmission distance 12 meters); A parallele port(for printer or floppy disk drive); A power supply for the floppy disk drive （only to be used with the power cable supplied by Mindray ）. B.7 Power supply Voltage: AC 100 V – 240 V;...
Page 306 Appendices B.9 Sound Maximal sound: 77 dB. B.10 Operating environment Operating temperature: 15 ℃ - 35 ℃; Optimal operating temperature: 15 ℃ - 30 ℃; Relative humidity: 30 % - 85 %; Atmospheric pressure: 70 kPa - 106 kPa. B.11 Storage environment Ambient temperature: -10 ℃...
Page 307: C Precautions, Limitations And Hazards
Precautions, Limitations and Hazards C.1 Introduction You will find the following symbols in this manual. When you see… Then… read the statement below the symbol. The statement is alerting you to an operating hazard that can cause personnel injury. read the statement below the symbol. The statement is alerting you to a possibility of analyzer damage or unreliable analysis results.
Page 308 Appendices C.2 Warnings It is important for the hospital or organization that employs this equipment to carry out a reasonable service/maintenance plan. Neglect of this may result in machine breakdown or injury of human health. Make sure the analyzer is properly grounded.。 Before turning on the analyzer, make sure the input voltage meets the above requirements.
Page 309 Do not perform any maintenance procedures that are not described in this chapter. Performing unauthorized maintenance procedures can damage your analyzer. In case of problems not specified in this manual, contact Mindray customer service department or your local distributor for assistance. Only Mindray-supplied parts can be used for maintenance.
Page 310 Appendices C.4 Notes This equipment must be operated by skilled/trained medical professionals Be sure to operate the analyzer strictly as instructed in this manual. This analyzer adopts a fixed decimal point. You can enter the digits without bothering to look for the [.] on the external keyboard. The purpose of this analyzer is to identify the normal patient, with all normal system-generated parameters, and to flag or identify patient results that require additional studies.
Page 311 Appendices If you do not need the PLT, MCV and WBC differential results, you can store the samples in a refrigerator (2℃ - 8℃) for 24 hours. You need to warm the refrigerated samples at room temperature for at least 30 minutes before running them.
Page 312 Appendices Review” screen. Refer to the instructions of use of the control for information on the lot number, expiration date, open-vial stability days, expected results and limits. The entered expiration date should be either the expiration date printed on the labeling or the open-vial expiration date, whichever is earlier. The open-vial expiration date is calculated as follows: the date that vial is opened + the open-vial stability days.
Page 313 If the recommended solution fails to solve the problem, contact Mindray customer service department or your local distributor. Unless otherwise instructed, always turn off the power before trying to fix the error.
Page 314 Appendices C.5 Biohazard Samples, controls, calibrators and waste are potentially infectious. Wear proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory. All the analyzer components and surfaces are potentially infectious, take proper protective measures for operation or maintenance.
Page 315 Appendices C.6 Abnormal Results For your reference only. C.6.1 Abnormal Sample Analysis Results Parameter flags If the analysis result is followed by an ”H” or “L”, it means the analysis result has exceeded the upper or lower limit of the reference range. If you see *** as opposed to the result, it means the result is either unreliable or out of the operating range.
Page 316 C.7 Abnormal QC Results In case of any abnormal QC results, do the following steps until the problem is solved. If all the steps have failed, contanct Mindray customer service department or your local distributor for assistance. Check the upper left corner of the screen for error messages. Refer to Chaper 11 Troublshooting Your Analyzer for solutions to any displayed error messages;...
Page 317: D Communication
Communication D.1 Introduction The BC-3000 Plus can transmit the sample data and QC data to an external computer (a host) through its RS-232 serial port. The transmission can be conducted either automatically or through the command of the operator after the completion of the sample analysis. This...
Page 318 DTR：Data Terminal Ready GND：Signal Ground DSR：Data Set Ready RTS：Request to Send CTS：Clear to Send RI：Ring Indicator The BC-3000 Plus communicates with a host through serial port 2, using Pin2, Pin 3 and Pin 5. The maximum transmission distance is 12 meters.
Page 319 Appendices D.3 Transmission Data Format D.3.1 Description Symbols [ENQ] 0x05 [STX] 0x02 [EOT] 0x04 [EOF] 0x1A [ETX] 0x03 [ACK] 0x06 [NACK] 0x15 "A" 0x41 "B" 0x42 "C" 0x43 "#" 0x30-0x39 "*" 0x2A If the Lot No., Month, Day, Year are empty in QC Edit menu, the “*” (2A Hex) will be transmitted to the host.
Page 320 If the Handshake is on, BC-3000 Plus will communicate with the external computer in following procedures: 1. BC-3000 Plus sends an ENQ (05 Hex), then waits up to 4 seconds for the external computer to respond. If the external computer does not respond, then one more ENQ (05 Hex) is tried.
Page 321 Appendices If the external compute responds ACK, the transmission is done successfully. If the external computer responds NACK（15 Hex）, the analyzer repeat the transmission from step 3. If the received response from the computer is neither ACK（06 Hex） nor NACK（15 Hex）, the analyzer sends ETX(03 Hex) again.
Page 322 Appendices RDW-SD[fL] ###.# Reserved ############ L1 Region L2 Region L3 Region L4 Region L5 Region L6 Region L7 Region L8 Region Reserved ################ WBC Histo (256 channels) RBC Histo (256 channels) PLT Histo (256 channels) Body of the text end If handshake is enabled [EOT] If handshake is disabled...
Page 323 Appendices Gran#[10 ###.# Gran%[%] ##.# HCT[%] ##.# MCV[fL] ###.# MCH[pg] ###.# MCHC[g/L] #### WBC Limit[10 ###.# RBC Limit[10 #.## HGB Limit[g/L] PLT Limit[10 #### Lymph# Limit[10 ###.# Lymph% Limit[%] ##.# Gran# Limit[10 ###.# Gran% Limit[%] ##.# HCT Limit[%] ##.# MCV Limit[fL] ###.# MCH Limit[pg] ###.#...
Page 324 Appendices MCV[fL] ###.# MCH[pg] ###.# MCHC[g/L] #### Body of the text end If handshake is enabled [EOT] If handshake is disabled [EOF] If handshake is enabled [ETX] D.4 Transmission D.4.1 Defining Transmission Settings The data format is fixed for the transmission so that every byte to be transmitted has 7 data bits and 1 stop bit.
Page 325 P/N:3003-20-34858...

Mindray BC-3000 Plus Operation Manual

1 Using this Manual

2 Understanding Your Analyzer

3 Understanding the System Principles

4 Installing Your Analyzer

5 Customizing the Analyzer Software

6 Operating Your Analyzer

7 Reviewing Sample Results

8 Using the QC Programs

9 Using the Calibration Programs

10 Maintaining Your Analyzer

11 Troubleshooting Your Analyzer

12 Appendices

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