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9.3 How to Calibrate
The analyzer provides 3 calibration programs: manual calibration, calibration using
commercial calibrator and calibration using fresh blood samples. Two sets of calibration
factors are prepared respectively for the whole blood mode and the predilute mode.
9.3.1 Preparing Your Analyzer
Do the following pre-calibration procedures before calibration. If problems are detected
during these checks, do not attempt to calibrate the analyzer. If necessary, call Mindray
customer service department or your local distributor for assistance.
Check and make sure there are enough reagents for the calibration. You need to start over
the calibration if the reagents run out during the process.
Do the background check. If the analyzer alarms for abnormal background results, see
Chapter 11 Troubleshooting Your Analyzer for solutions.
Enter the "Count" screen and run a vial of normal control 11 consecutive times. Enter the
"Review" screen to check the reproducibility of the second to eleventh runs and make sure
they meet the following requirements.
Parameter
WBC
RBC
HGB
MCV
PLT
At the "Count" screen, run a vial of high control three consecutive times and then
immediately run the diluent three consecutive times, calculate the carryover per the following
equation.
First
=
Carryover
(%)
T
hird
The calculated carryovers shall meet the following requirements: WBC, RBC and HGB shall
be no greater than 0.5 % ; PLT shall be no greater than 1%.
It is recommended that you create a log table for your analyzer. This log table should contain
Using the Calibration Programs
Table 9-1 Reproducibility
Expected range
4.0 to 15.0 × 10
3.00 to 6.50 × 10
100 to 180 g/L
70.0 to 100.0 fL
200 to 500 × 10
low
-
level
sample
result
high
-
level
sample
result
9-3
9
≤ 3.0
/ L
12
≤ 2.0
/ L
≤ 2.0
≤ 1.0
9
/ L
≤ 5.0
－
T
hird
low
-
level
－
T
hird
low
-
level
CV(%)
sample
result
×
％
100
sample
result
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