Determining The Backpressure Of The Flow Cell - Thermo Scientific Vanquish VH-D10 Operating Manual

5 • Installation
5.8.4

Determining the Backpressure of the Flow Cell

Page 78
This procedure first describes how to measure the system backpressure
with flow cell but without additional module (p4). The backpressure of
the flow cell (p5) is then calculated by subtracting the Vanquish system
backpressure without flow cell and additional module (p1).
Figure 28: Measuring the backpressure of the flow cell, column, detector
inlet capillary and waste line
No. Description
1 Detector
2 Flow cell
2a Outlet port of the flow cell
2b Inlet port of the flow cell
1. Set up the flow connections as described in
Flow Cell (} page 66).
2. Start the pump flow at the flow rate of your application.
3. Check all flow connections for leakages:
If a leakage has occurred: See
¨
(} page 170).
If no leakage was found and the pressure value has stabilized:
¨
Proceed with the next step.
4. When the system pressure has stabilized, read the system pressure
and write down the value for p4.
p4: System backpressure with flow cell but without additional
module
5. Stop the pump flow.
No. Description
3 Detector inlet capillary or
overpressure relief valve with
detector inlet capillary
4 Waste container
5 Waste line
Flow Connections to the
Resolving Liquid Leaks
Diode Array Detectors (VH-D10)
Operating Manual