Thermo Scientific Vanquish VH-D10 Operating Manual page 137

Diode Array Detectors (VH-D10)
Operating Manual
3. Disconnect the flow cell capillary from the back-flush capillary (no.
1e) and screw the back-flush plug (no. 3) into to the T piece (no. 1d).
The image below shows the finalized setup for determining the
pump flow.
Figure 39: Setup for determining the pump flow
No. Description
A To the outlet of the pump
B Plug
C Waste container
4. Set the upper pressure limit of the pump to 60% of the specified
pressure limit of the flow cell plus 0.5 MPa.
5. Turn on the pump flow at a flow rate of 0.1 mL/min with the back-
flushing agent.
6. Slowly increase the pump flow until the pump pressure reaches 60%
of the specified pressure limit of the flow cell and write down the
determined value. If the pump pressure does not reach the 60%, set
the maximum pump flow.
7. Stop the pump flow.
8. Flush the T piece (see the section below).
7 • Maintenance and Service
Page 137