Thermo Scientific Vanquish VH-D10 Operating Manual page 77

Diode Array Detectors (VH-D10)
Operating Manual
4. Connect the inlet port of the additional module to the free end of
the union connector with the transfer capillary which will be used to
connect the additional module in the application setup.
5. Start the pump flow at the maximum flow rate of your application.
6. Check all flow connections for leakages:
If a leakage has occurred: See
¨
(} page 170).
If no leakage was found and the pressure value has stabilized:
¨
Proceed with the next step.
7. When the system pressure has stabilized, read the system pressure
and write down the value for p2.
p2: System backpressure with the additional module, but with the
flow cell replaced by the union connector
8. Stop the pump flow.
9. Calculate the backpressure the connected additional module
produces at the flow cell outlet: p3 = p2 – (p1-p0).
p3: Backpressure the connected additional module produces at the
flow cell outlet
p2: System backpressure with the additional module, but with the
flow cell replaced by the union connector
p1: Vanquish system backpressure (without flow cell and additional
module)
p0: Backpressure caused by the waste line
10. Compare p3 with the pressure limit of the flow cell.
Situation
If p3 is well below the
pressure limit of your
flow cell
If p3 is close to the
pressure limit of your
flow cell or exceeds it
See also
2
Flow Cell Specifications (} page 174)
Resolving Liquid Leaks
Steps
Proceed with Determining the Backpressure of the Flow
Cell (} page 78).
Reduce the flow rate gradually and restart the
procedure until p3 is well below the pressure limit of
your flow cell.
–or–
Change the system configuration (for example, the
transfer capillary) and restart the procedure to check if
p3 is well below the pressure limit of your flow cell.
5 • Installation
Page 77