Thermo Scientific Vanquish VH-D10 Operating Manual page 108

6 • Operation
Page 108
Situation after If no additive is used
Shutdown
Device and flow cell Flush the system with
shall be transported isopropanol.
or shipped after
shutdown
NOTICE
Residual samples, impurities from the column or buffers with high salt
concentrations can deposit in the flow cell. This can lead to damage of
the flow cell. In addition, solvents containing acid can damage the flow
cell.
• Always flush the flow cell with an appropriate solvent before
interrupting operation.
• Fill the flow cell with pure isopropanol using the flushing and
injection kit.
• The flow cell should not be filled with pure water to avoid the
growth of algae. If you want to fill the flow cell with water, you need
to add 10% HPLC-grade isopropanol.
6. Turn off the pump flow to the device. Wait until the system pressure
is down to zero before you continue the shutdown of the device.
7. Disconnect the capillaries from the flow cell inlet and outlet.
8. After removing the flow connections, protect the flow cell inlet and
outlet ports with the plugs supplied with the flow cell. Protect the
inlet capillaries with caps.
If an additive is used
Flush the system first with
several volumes of methanol
and water (50:50) (for
example, 1.0 mL/min for
10 minutes with the standard
system) to prevent salt buildup
in the fluidics. If the solvents in
the flow cell are not miscible
with water, use an appropriate
intermediate solvent.
Afterward, flush the system
with isopropanol.
Diode Array Detectors (VH-D10)
Operating Manual