Checking The Flow Cell - Thermo Scientific Vanquish VH-D10 Operating Manual

8.3

Checking the Flow Cell

When
Follow these steps
Diode Array Detectors (VH-D10)
Operating Manual
Related messages that require a flow cell check appear in the Audit Trail.
1. Check that the flow cell is installed properly.
2. Remove and re-install the flow cell.
3. Flush the flow cell with pure HPLC-grade water with a flow rate of
>1 mL/min for several minutes.
4. Install a different flow cell and repeat the action during which the
message appeared with the flow cell that was previously installed.
Result
If the message disappears
with a different flow cell
If the message still
appears
See also
2
Messages (} page 164)
Steps
The problems are caused by the flow cell that was
previously installed or the flow path or components in
the flow path before the detector. If you suspect a
contamination or an elevated backpressure in the flow
cell, perform a back-flushing procedure with the flow
cell that was previously installed (see
Flow Cell (} page 134)).
The problem is caused by the flow path or components
in the flow path before the detector or by the detector.
Install a diagnostic cell and repeat the action during
which the message appeared with the flow cell that was
previously installed.
• If the message disappears with the diagnostic cell: The
problems are caused by the flow path or components
in the flow path before the detector.
• If the message still appears: The problems are caused
by the detector.
8 • Troubleshooting
Back-Flushing the
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