Thermo Scientific Vanquish VH-D10 Operating Manual page 99

Diode Array Detectors (VH-D10)
Operating Manual
Parameters Description
Autozero Perform an automatic null balancing. The current device signal is
interpreted as 0. Therefore, no absorbing sample should be in
the flow cell when Autozero is performed.
TIP When you start the data acquisition or perform an autozero,
the shutter in the light path before the flow cell is opened
automatically. After the data acquisition, close the shutter, if
required.
Leak detection Leak detection is enabled as a standard when the device is
shipped (Leak Sensor Mode = Enabled). This is the preferred
setting.
Wavelength Perform the wavelength validation as required. A holmium-oxide
validation glass filter is moved into the light path of the lamp and is used to
validate the wavelength accuracy. For further information, see
Performing a Wavelength Validation and Wavelength Calibration
(} page 120).
Wavelength If the wavelength validation failed, perform a calibration of
calibration wavelengths. During the calibration, the device determines the
measured wavelength of the D-alpha line of the UV lamp. If the
device determines that the D-alpha line differs from the
expected value, the device will adjust its wavelength calibration.
For further information, see
and Wavelength Calibration
Lamphouse Two temperature signal channels are available in the Properties
temperature dialog box of the device: Lamphouse Temperature and
Spectrograph Temperature. The lamp and the spectrograph of
‑and‑
the device are very sensitive (regarding drift) to changes in
Spectrograph
temperature. The temperature signal channels can be used for
temperature
troubleshooting purposes:
In case of a long-term baseline drift, a varying lamp house
temperature signal indicates strong temperature variations.
In case of a long-term baseline drift and a constant lamp house
temperature, a changing spectrograph temperature indicates a
device defect (defective optics insulation or defective
spectrograph temperature control).
Select one or both signal channels to record the temperatures.
Chromeleon generates the appropriate channels for recording
the temperatures of the device lamp house and spectrograph.
6 • Operation
Performing a Wavelength Validation
(} page 120).
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