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6
•
Operation
6.9
Optimizing the Performance of the Device
6.9.1
Guidelines for Optimum Performance
Page 100
This section provides information for best performance of the device
and gives hints on what you can do to optimize the performance further.
Consider the following guidelines for optimization of the device
performance:
•
The recording of up to 10 signal channels at individual wavelengths
enables a selective detection with narrow bandwidths. In a selective
detection, the UV spectrum for a particular species is recorded.
Determine an appropriate absorbance maximum.
Avoid the wavelength range where the solvents absorb strongly (for
example, below 220 nm for methanol and below 210 nm for
acetonitrile).
•
Ignite the UV lamp only if required. Igniting the UV lamp frequently
reduces the lifetime of the lamp. In addition, some time is required
for the detector to stabilize after a (re-)ignition of the lamp.
•
Monitor the lamp age and schedule appropriate maintenance
intervals.
•
Ensure that the operating conditions are suitable. This includes:
Stable environmental conditions, such as a stable temperature
¨
No air drafts
¨
No vibrations or mechanical shocks caused by external sources
¨
No EMC-related sources of strong interference. Operate only
¨
certified laboratory equipment in close proximity to the
detector.
Stable backpressure and correct waste line setup
¨
•
Monitor the usage of device-specific components that are subject to
wear and stress and schedule appropriate maintenance intervals
(see
Predictive Performance
•
Observe the general guidelines and recommendations on the use of
solvents and additives in the chromatography system. Refer to Use
of Solvents and Additives in the Vanquish System Operating Manual.
•
Degas the solvent.
•
Consider the influences of the parameters on the detection.
•
Keep the device doors closed during operation to avoid exposure of
the flow cell to thermal changes in the environment that may lead to
a baseline drift.
(} page 119)).
Diode Array Detectors (VH-D10)
Operating Manual
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